Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F1 I2 R1
|
78 |
48.0 |
2243616 |
94.8% |
2126947 |
114.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,830,989 |
T→C |
V178A (GTT→GCT) |
yicH → |
putative inner membrane‑anchored periplasmic AsmA family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,830,989 | 0 | T | C | 100.0%
| 99.2
/ NA
| 27 | V178A (GTT→GCT) | yicH | putative inner membrane‑anchored periplasmic AsmA family protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/14); total (13/14) |
CGAATAGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGTTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGGGTGCCGACATCGCCCGCGGGACATTAACCGGAAACGCGCAGCGTAACGCCGACGGCAGCTGGCAAGT > NC_000913/3830857‑3831121
|
cGAATAGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGc > 1:340305/1‑139 (MQ=255)
cGAATAGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGc < 1:464065/139‑1 (MQ=255)
aaTAGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGcca < 1:138795/139‑1 (MQ=255)
aaTAGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGcca > 2:531019/1‑139 (MQ=255)
aTAGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGccac < 2:340305/139‑1 (MQ=255)
aCTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGAtt > 2:18168/1‑139 (MQ=255)
aGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAg < 2:767212/139‑1 (MQ=255)
aGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAg > 1:967104/1‑139 (MQ=255)
cgcgcAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGc < 1:412003/139‑1 (MQ=255)
cggcgTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGc > 1:1062941/1‑121 (MQ=255)
cggcgTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGc < 2:1062941/121‑1 (MQ=255)
ggcgTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTAc > 1:338337/1‑109 (MQ=255)
ggcgTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTAc < 2:338337/109‑1 (MQ=255)
ggcgTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAG‑TTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAgg < 1:578747/118‑1 (MQ=255)
ggcgTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAG‑TTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAgg > 2:578747/1‑118 (MQ=255)
aCCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACg > 1:123281/1‑127 (MQ=255)
aCCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACg < 2:123281/127‑1 (MQ=255)
ggTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGc > 1:866789/1‑71 (MQ=255)
ggTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGc < 2:866789/71‑1 (MQ=255)
ggTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGa > 1:930359/1‑77 (MQ=255)
ggTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGa < 2:930359/77‑1 (MQ=255)
cAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCa > 1:611051/1‑70 (MQ=255)
cAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCa < 2:611051/70‑1 (MQ=255)
cAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGTGTGCCGACATCGCCCGCGGGACATTAACCGGaa > 2:155249/1‑139 (MQ=255)
aTCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGGGTGCCGACATCGCCCGCGGGACATTAACCGGAAACGCGCAGCGTAACGCCGAc < 1:74086/139‑1 (MQ=255)
aTCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGGGTGCCGACATCGCCCGCGGGACATTAACCGGAAACGCGCAGCGTAACGCCGAc < 1:18168/139‑1 (MQ=255)
atgatgCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGGGTGCCGACATCGCCCGCGGGACATTAACCGGAAACGCGCAGCGTAACGCCGACGGCAGCTGGCAAGt > 2:579695/1‑139 (MQ=255)
|
CGAATAGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGTTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGGGTGCCGACATCGCCCGCGGGACATTAACCGGAAACGCGCAGCGTAACGCCGACGGCAGCTGGCAAGT > NC_000913/3830857‑3831121
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
