Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I2 R1	78	48.0	2243616	94.8%	2126947	114.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,293,898	A→G	Y119C (TAC→TGC)	nrfG →	heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,293,898	0	A	G	100.0%	116.4 / NA	31	Y119C (TAC→TGC)	nrfG	heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/18); total (13/18)

GAACAGTGGGCGTTACTGGGCGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCA  >  NC_000913/4293762‑4294027

gAACAGTGGGCGTTACTGGGCGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGcc                                                                                                                                 <  1:507667/139‑1 (MQ=255)

     gTGGGCGTTACTGGGCGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGgcc                                                                                                                            <  1:7755/139‑1 (MQ=255)

        ggCGTTACTGGGCGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGgccagc                                                                                                                         <  2:843743/139‑1 (MQ=255)

                tGGGCGAGTACTATCTGTGGCAAAACGATTACAGTAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCatat                                                                                                                 <  2:718970/139‑1 (MQ=255)

                  ggCGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGa                                                                                                               <  2:877413/139‑1 (MQ=255)

                             tCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGATTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACt                                                                                                    <  2:1119027/139‑1 (MQ=255)

                                  ggCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACt                                                                                                    >  2:354019/1‑134 (MQ=255)

                                  ggCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACt                                                                                                    <  1:354019/134‑1 (MQ=255)

                                                                      gTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGa                                                           <  2:265147/139‑1 (MQ=255)

                                                                            cGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCAc                                                     <  2:1007679/139‑1 (MQ=255)

                                                                                gCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGcc                                                 >  1:1039276/1‑139 (MQ=255)

                                                                                       cGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGAtgc                                          >  2:176441/1‑139 (MQ=255)

                                                                                       cGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGAtgc                                          >  2:119190/1‑139 (MQ=255)

                                                                                       cGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAACCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGAtgc                                          >  2:769184/1‑139 (MQ=255)

                                                                                         tggtgAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGAtgctg                                        <  2:1042397/139‑1 (MQ=255)

                                                                                           gtgAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATgctgct                                      >  2:21677/1‑139 (MQ=255)

                                                                                               gaACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATCATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCt                                  >  1:659933/1‑139 (MQ=255)

                                                                                                aaCGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTcgcg                                                                         <  2:814070/99‑1 (MQ=255)

                                                                                                aaCGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTcgcg                                                                         >  1:814070/1‑99 (MQ=255)

                                                                                                aaCGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGa                                                                    <  1:578610/104‑1 (MQ=255)

                                                                                                aaCGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGa                                                                    >  2:578610/1‑104 (MQ=255)

                                                                                                    cTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAACCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGa                             <  1:769184/139‑1 (MQ=255)

                                                                                                             aTGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGa                                                                                          >  2:69606/1‑69 (MQ=255)

                                                                                                             aTGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGa                                                                                          <  1:69606/69‑1 (MQ=255)

                                                                                                             aTGCGGCGCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTAt                    >  2:100958/1‑139 (MQ=255)

                                                                                                                 ggcgCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTcg                                                                           <  2:713748/80‑1 (MQ=255)

                                                                                                                 ggcgCTGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTcg                                                                           >  1:713748/1‑80 (MQ=255)

                                                                                                                      tGGCGACGGTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGaa           >  1:282522/1‑139 (MQ=255)

                                                                                                                             ggTGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTAcgcg    <  2:33664/139‑1 (MQ=255)

                                                                                                                               tGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCa  <  1:517435/139‑1 (MQ=255)

                                                                                                                               tGCTTTATTGCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCa  <  2:282522/139‑1 (MQ=255)

GAACAGTGGGCGTTACTGGGCGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCA  >  NC_000913/4293762‑4294027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A