Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	57	49.8	2216334	94.8%	2101084	113.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,572,786	A→G	V806A (GTT→GCT)	pqqL ←	putative periplasmic M16 family zinc metalloendopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,572,786	0	A	G	100.0%	208.0 / NA	55	V806A (GTT→GCT)	pqqL	putative periplasmic M16 family zinc metalloendopeptidase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/33); total (22/33)

GATCCCTTTAGCCAGACGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAACGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCAC  >  NC_000913/1572650‑1572914

gATCCCTTTAGCCAGACGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCg                                                                                                                                <  1:201376/139‑1 (MQ=255)

gATCCCTTTAGCCAGACGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCg                                                                                                                                <  1:481979/139‑1 (MQ=255)

 aTCCCTTTAGCCAGACGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCga                                                                                                                               >  2:384233/1‑138 (MQ=255)

       ttAGCCAGACGCTTAACCATCACTTCAATCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATg                                                                                                                         <  1:1084639/139‑1 (MQ=255)

         aGCCAGACGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCt                                                                                                                       >  1:333484/1‑139 (MQ=255)

               aCGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGAt                                                                                                                 >  1:454980/1‑139 (MQ=255)

               aCGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGAt                                                                                                                 >  1:947823/1‑139 (MQ=255)

               aCGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGAt                                                                                                                 >  1:646257/1‑139 (MQ=255)

                 gCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGc                                                                                                               <  2:599948/139‑1 (MQ=255)

                        cATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCa                                                                                                        <  2:811387/139‑1 (MQ=255)

                         aTCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCAc                                                                                                       <  1:515312/139‑1 (MQ=255)

                         aTCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCAc                                                                                                       <  1:458027/139‑1 (MQ=255)

                          tCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGc                                                                                                               >  2:292051/1‑130 (MQ=255)

                          tCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGc                                                                                                               <  1:292051/130‑1 (MQ=255)

                          tCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACg                                                                                                      <  2:646257/139‑1 (MQ=255)

                          tCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACg                                                                                                      <  1:984182/139‑1 (MQ=255)

                           cACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGa                                                                                                     <  2:355388/139‑1 (MQ=255)

                                 aTTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTa                                                                                               <  2:947823/139‑1 (MQ=255)

                                      cTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATAATAACACg                                                                                          >  2:249402/1‑139 (MQ=255)

                                        aaCGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTa                                                                                        >  1:760927/1‑139 (MQ=255)

                                        aaCGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTa                                                                                        <  1:249402/139‑1 (MQ=255)

                                        aaCGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTa                                                                                        <  1:169694/139‑1 (MQ=255)

                                               aCAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATg                                                                                                                         <  1:254273/99‑1 (MQ=255)

                                               aCAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATg                                                                                                                         >  2:254273/1‑99 (MQ=255)

                                                    tcatcaTGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGACGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTTCCa                                                                            >  1:627594/1‑139 (MQ=255)

                                                     catcatGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATg                                                                                                                         >  1:287354/1‑93 (MQ=255)

                                                     catcatGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATg                                                                                                                         <  2:287354/93‑1 (MQ=255)

                                                     catcatGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCAAg                                                                           >  1:275552/1‑139 (MQ=255)

                                                       tcatGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTg                                                                         <  2:333484/139‑1 (MQ=255)

                                                        catGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGc                                                                        <  2:275552/139‑1 (MQ=255)

                                                         atGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCg                                                                       <  1:250867/139‑1 (MQ=255)

                                                         atGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCg                                                                       <  1:11619/139‑1 (MQ=255)

                                                           gTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGAc                                                                     <  2:454980/139‑1 (MQ=255)

                                                               ttCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCt                                                                                                                              <  2:397278/78‑1 (MQ=255)

                                                               ttCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCt                                                                                                                              >  1:397278/1‑78 (MQ=255)

                                                               ttCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCt                                                                                                                              <  2:266731/78‑1 (MQ=255)

                                                               ttCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCt                                                                                                                              >  1:266731/1‑78 (MQ=255)

                                                                       gACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATg                                                                                                                         >  2:200710/1‑75 (MQ=255)

                                                                       gACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATg                                                                                                                         <  1:200710/75‑1 (MQ=255)

                                                                        aCAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCg                                                        <  2:760927/139‑1 (MQ=255)

                                                                                 aGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCAGCGAGCGCCATa                                               >  1:717290/1‑139 (MQ=255)

                                                                                      gCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGc                                                                                                               >  2:975672/1‑70 (MQ=255)

                                                                                      gCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGc                                                                                                               <  1:975672/70‑1 (MQ=255)

                                                                                       cAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCa                                                 >  2:1025544/1‑131 (MQ=255)

                                                                                       cAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCa                                                 <  1:1025544/131‑1 (MQ=255)

                                                                                          aTGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGc                                      <  2:717290/139‑1 (MQ=255)

                                                                                                 atatCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAGCg                                                                                                                                <  1:264107/42‑1 (MQ=255)

                                                                                                               aGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCgg                                       >  2:468370/1‑117 (MQ=255)

                                                                                                               aGGATCAACCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCgg                                       <  1:468370/117‑1 (MQ=255)

                                                                                                                     aaCCGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATaa           >  2:1086659/1‑139 (MQ=255)

                                                                                                                        cGAGAGGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGc        <  1:948534/139‑1 (MQ=255)

                                                                                                                           gagGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATAtt                                                                                                <  1:935473/48‑1 (MQ=255)

                                                                                                                           gagGCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATAtt                                                                                                >  2:935473/1‑48 (MQ=255)

                                                                                                                            agtcGAGAAGAAGTGCTGTATGCGCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAATAGAATCGAGCGCCATACGCCTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTcc    <  2:730155/136‑1 (MQ=255)

                                                                                                                              gCGAGAAGAAGCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCAc  >  1:1012459/1‑139 (MQ=255)

GATCCCTTTAGCCAGACGCTTAACCATCACTTCATTCGCTAACGTTAACAGTTCATCATGTCGTTCTGGTTGACAAGTAAAAGCCAGCAAATGACTGATATCTTTGGCCTGAGGATCAACCGAGAGGCGAGAAGAAACGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCAC  >  NC_000913/1572650‑1572914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A