Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F1 I1 R1 57 49.8 2216334 94.8% 2101084 113.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,244,769 A→G intergenic (+23/+9) yeiG → / ← cirA S‑formylglutathione hydrolase/colicin IA outer membrane receptor and translocator, ferric iron‑catecholate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,244,7690AG100.0% 231.9 / NA 61intergenic (+23/+9)yeiG/cirAS‑formylglutathione hydrolase/colicin IA outer membrane receptor and translocator, ferric iron‑catecholate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/25);  total (36/25)

CGTATTCAGCCGGGATATGATCACAGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTAGTC  >  NC_000913/2244654‑2244901
                                                                                                                   |                                                                                                                                    
cGTATTCAGCCGGGATATGATCACAGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGtc                                                                                                                                    >  1:328373/1‑118 (MQ=255)
        gCCGGGATATGATCACAGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCa                                                                                                       <  1:775829/139‑1 (MQ=255)
                       caGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGAcgtc                                                                                        >  2:517461/1‑139 (MQ=255)
                       caGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGAAGTATTTACTGAAGGGAAAGTCCGCCCGGTTCGCCGTGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGAcgtc                                                                                        >  2:646950/1‑139 (MQ=255)
                        aGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGAcgtcc                                                                                       <  2:933518/139‑1 (MQ=255)
                            actactTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCt                                                                                   >  2:66599/1‑139 (MQ=255)
                             ctactTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGACGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCtt                                                                                  >  2:29765/1‑139 (MQ=255)
                                  tCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTta                                                                             >  2:372365/1‑139 (MQ=255)
                                  tCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTta                                                                             >  1:931903/1‑139 (MQ=255)
                                    aTCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTtata                                                                           >  2:1048174/1‑139 (MQ=255)
                                    aTCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTtata                                                                           >  2:456954/1‑139 (MQ=255)
                                        ccTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTg                                                                       >  2:851472/1‑139 (MQ=255)
                                           ctTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCa                                                                                                       <  2:137660/104‑1 (MQ=255)
                                           ctTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCa                                                                                                       <  2:529264/104‑1 (MQ=255)
                                           ctTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCa                                                                                                       >  1:529264/1‑104 (MQ=255)
                                           ctTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCa                                                                                                       >  1:137660/1‑104 (MQ=255)
                                                   gagGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACg                                                            <  1:29765/139‑1 (MQ=255)
                                                      gATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCtcatc                                                                                                                            <  1:539041/72‑1 (MQ=255)
                                                      gATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCtcatc                                                                                                                            >  2:539041/1‑72 (MQ=255)
                                                      gATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCAc                                                                                                            <  2:653942/88‑1 (MQ=255)
                                                      gATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCAc                                                                                                            >  1:653942/1‑88 (MQ=255)
                                                      gATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTtat                                                                            <  2:851306/120‑1 (MQ=255)
                                                      gATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTtat                                                                            >  1:851306/1‑120 (MQ=255)
                                                      gATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACt                                                         >  1:651101/1‑139 (MQ=255)
                                                         cACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTgag                                                      >  2:369749/1‑139 (MQ=255)
                                                                   tCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCg                                            >  1:500060/1‑139 (MQ=255)
                                                                     cATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGcc                                          <  1:487220/139‑1 (MQ=255)
                                                                      aTGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCa                                                                                                       <  1:282974/77‑1 (MQ=255)
                                                                      aTGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCa                                                                                                       >  2:282974/1‑77 (MQ=255)
                                                                      aTGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCa                                         <  2:651101/139‑1 (MQ=255)
                                                                           cAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGtt                                    <  1:369749/139‑1 (MQ=255)
                                                                            aGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATc                                                                                                               <  2:484637/63‑1 (MQ=255)
                                                                            aGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATc                                                                                                               >  1:484637/1‑63 (MQ=255)
                                                                              tATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCa                                                                                                       <  2:466074/69‑1 (MQ=255)
                                                                              tATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCa                                                                                                       >  1:466074/1‑69 (MQ=255)
                                                                              tATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAgg                                      >  2:296162/1‑134 (MQ=255)
                                                                              tATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAgg                                      <  1:296162/134‑1 (MQ=255)
                                                                              tATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAg                                 >  2:1048746/1‑139 (MQ=255)
                                                                                      gAAGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTg                         >  2:913201/1‑139 (MQ=255)
                                                                                        aGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGa                                                                                                                         >  1:1056106/1‑41 (MQ=255)
                                                                                        aGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGa                                                                                                                         <  2:1056106/41‑1 (MQ=255)
                                                                                        aGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCa                                                                                                             >  2:477713/1‑53 (MQ=255)
                                                                                        aGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCa                                                                                                             <  1:477713/53‑1 (MQ=255)
                                                                                        aGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAgg                                      >  2:722844/1‑124 (MQ=255)
                                                                                        aGTGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAgg                                      <  1:722844/124‑1 (MQ=255)
                                                                                          tGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCAc                                                                                                            <  2:731334/52‑1 (MQ=255)
                                                                                          tGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCAc                                                                                                            >  2:89347/1‑52 (MQ=255)
                                                                                          tGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCAc                                                                                                            <  1:89347/52‑1 (MQ=255)
                                                                                          tGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCAc                                                                                                            >  1:731334/1‑52 (MQ=255)
                                                                                          tGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTgcgcg                     >  2:4334/1‑139 (MQ=255)
                                                                                          tGAAAGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTgcgcg                     >  2:281599/1‑139 (MQ=255)
                                                                                            aaaGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGtt                                    <  2:362933/122‑1 (MQ=255)
                                                                                            aaaGTCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGtt                                    >  1:362933/1‑122 (MQ=255)
                                                                                                tCCGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGttt               <  1:1048174/139‑1 (MQ=255)
                                                                                                  cGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGa             >  1:286888/1‑139 (MQ=255)
                                                                                                  cGCCCGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGa             >  1:851426/1‑139 (MQ=255)
                                                                                                    cccGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCtt                                                                                  <  1:1009556/68‑1 (MQ=255)
                                                                                                    cccGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCtt                                                                                  >  2:1009556/1‑68 (MQ=255)
                                                                                                    cccGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCa                               >  2:372379/1‑119 (MQ=255)
                                                                                                    cccGGTTCGCCGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCa                               <  1:372379/119‑1 (MQ=255)
                                                                                                             ccGGGCGTCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTAGTc  <  1:210925/139‑1 (MQ=255)
                                                                                                                   |                                                                                                                                    
CGTATTCAGCCGGGATATGATCACAGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTAGTC  >  NC_000913/2244654‑2244901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A