Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
57 |
49.8 |
2216334 |
94.8% |
2101084 |
113.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,621,471 |
G→A |
A197T (GCC→ACC) |
deoD → |
purine nucleoside phosphorylase 1, nicotinamide 1‑beta‑D‑riboside phosphorylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,621,471 | 0 | G | A | 100.0%
| 141.7
/ NA
| 43 | A197T (GCC→ACC) | deoD | purine nucleoside phosphorylase 1, nicotinamide 1‑beta‑D‑riboside phosphorylase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/22); total (21/22) |
TAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTG > NC_000913/4621338‑4621605
|
tAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAACCCTg < 2:521174/139‑1 (MQ=255)
tAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAACCCTg < 1:569233/139‑1 (MQ=255)
tcCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCa < 2:80745/139‑1 (MQ=255)
cTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGt > 2:863927/1‑139 (MQ=255)
ctctCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATTCGCGGCGTGGAAATGGAAGCGGCTGGTATCTAAGGCGTCGCTGCAGAATTTGGCGCGAAAAGCCTGACCATCTGCACCGTATCTGACAACAta > 2:660376/1‑138 (MQ=255)
ctcCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCg < 1:1030522/139‑1 (MQ=255)
cGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCa > 2:732524/1‑117 (MQ=255)
cGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCa < 1:732524/117‑1 (MQ=255)
cGGCGAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGATGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCAc < 2:714124/139‑1 (MQ=255)
cGAAATGTGCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAg < 2:672071/139‑1 (MQ=255)
gAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCt < 2:540912/117‑1 (MQ=255)
gAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCt > 1:540912/1‑117 (MQ=255)
gATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCt > 1:1051745/1‑139 (MQ=255)
tctcGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCg > 1:540021/1‑96 (MQ=255)
tctcGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCg < 2:540021/96‑1 (MQ=255)
ctcGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCa < 2:1051745/139‑1 (MQ=255)
cGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGAc < 1:863927/139‑1 (MQ=255)
tGGATATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTg < 2:722234/67‑1 (MQ=255)
tGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATATGGCGCGAAAACCCTGACCATCTg > 1:722234/1‑67 (MQ=255)
ggCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCa > 1:359627/1‑139 (MQ=255)
gCGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCAc < 1:122400/43‑1 (MQ=255)
gCGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCAc > 2:122400/1‑43 (MQ=255)
cGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGc > 2:1054275/1‑89 (MQ=255)
cGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGc < 1:1054275/89‑1 (MQ=255)
cGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAAt < 1:888566/131‑1 (MQ=255)
cGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAAt > 2:888566/1‑131 (MQ=255)
cGTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCACACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTctg > 2:88847/1‑139 (MQ=255)
gTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGAc < 2:202820/51‑1 (MQ=255)
gTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGAc > 1:202820/1‑51 (MQ=255)
gTCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTctgc > 1:374811/1‑139 (MQ=255)
tCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCa > 2:1104932/1‑102 (MQ=255)
tCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCa < 1:1104932/102‑1 (MQ=255)
tCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTctgct > 2:17321/1‑139 (MQ=255)
tCGCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCACACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTctgct < 1:88847/139‑1 (MQ=255)
gCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTAt < 1:174986/43‑1 (MQ=255)
gCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTAt > 2:174986/1‑43 (MQ=255)
gCTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTgg < 1:17321/139‑1 (MQ=255)
cTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTggg > 1:949802/1‑139 (MQ=255)
cTGCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTggg > 2:813963/1‑139 (MQ=255)
gCAGAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCg < 1:660376/139‑1 (MQ=255)
gAATTTGGCGCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATa < 2:914567/139‑1 (MQ=255)
ggCTCGAAAACCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTTAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGt > 2:711430/1‑139 (MQ=255)
gAAAACCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATtg > 2:580653/1‑139 (MQ=255)
|
TAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTG > NC_000913/4621338‑4621605
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
