Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I1 R1
|
82 |
28.6 |
1225488 |
97.4% |
1193625 |
116.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,792,702 |
T→C |
A10A (GCA→GCG) |
nlpC ← |
putative C40 clan peptidase lipoprotein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,792,702 | 0 | T | C | 100.0%
| 111.2
/ NA
| 30 | A10A (GCA→GCG) | nlpC | putative C40 clan peptidase lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/16); total (14/16) |
CGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCAT > NC_000913/1792588‑1792829
|
cGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGaaa > 2:124429/1‑139 (MQ=255)
tAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTcc < 1:591110/139‑1 (MQ=255)
aGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCct < 1:124429/139‑1 (MQ=255)
aGCCGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCct < 2:393503/139‑1 (MQ=255)
gTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCa > 1:448684/1‑111 (MQ=255)
gTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCa < 2:448684/111‑1 (MQ=255)
gTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGtt > 1:577008/1‑139 (MQ=255)
aaaGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTAcc < 2:456941/139‑1 (MQ=255)
cTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGAtt < 2:519707/139‑1 (MQ=255)
gcggTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCt > 2:519609/1‑95 (MQ=255)
gcggTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCt < 1:519609/95‑1 (MQ=255)
tggtggCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAAc < 1:607825/52‑1 (MQ=255)
tggtggCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAAc > 2:607825/1‑52 (MQ=255)
tggCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCa > 1:532759/1‑52 (MQ=255)
tggCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCa < 2:532759/52‑1 (MQ=255)
ggCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATCAGATGGTc < 2:577008/139‑1 (MQ=255)
gCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATCAGATGGTCg > 2:185525/1‑139 (MQ=255)
gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTc < 1:542834/53‑1 (MQ=255)
gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTc > 2:542834/1‑53 (MQ=255)
gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCctc > 1:53804/1‑56 (MQ=255)
gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCctc < 2:53804/56‑1 (MQ=255)
gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCt > 1:560384/1‑69 (MQ=255)
gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCt < 2:560384/69‑1 (MQ=255)
gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTAcc < 2:303745/95‑1 (MQ=255)
gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTAcc > 1:303745/1‑95 (MQ=255)
gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGc > 2:201249/1‑137 (MQ=255)
gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGc < 1:201249/137‑1 (MQ=255)
gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCAt > 2:146839/1‑139 (MQ=255)
gCCAGAAGCAGCGCTGTGATC‑AAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGcg > 2:529799/1‑83 (MQ=255)
gCCAGAAGCAGCGCTGTGATC‑AAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGcg < 1:529799/83‑1 (MQ=255)
|
CGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCAT > NC_000913/1792588‑1792829
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
