Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I1 R1 82 28.6 1225488 97.4% 1193625 116.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,939,718 G→A A159V (GCG→GTG)  lpxM ← myristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,939,7180GA100.0% 53.5 / NA 17A159V (GCG→GTG) lpxMmyristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/9);  total (8/9)

AGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTC  >  NC_000913/1939587‑1939821
                                                                                                                                   |                                                                                                       
aGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCACTGCCAt                                                                                                  >  1:191099/1‑139 (MQ=255)
aGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCACTGCCAt                                                                                                  >  1:124428/1‑139 (MQ=255)
  tACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCACTGCCAttt                                                                                                <  2:610192/139‑1 (MQ=255)
  tACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCACTGCCAttt                                                                                                >  2:155266/1‑139 (MQ=255)
                cGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCACTGCCATTTTCTGCCCTTGCGAg                                                                                  <  2:124428/139‑1 (MQ=255)
                              gTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCACTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGc                                                                    <  1:507981/139‑1 (MQ=255)
                                ttAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCACTGCCAtttt                                                                                               >  1:555218/1‑110 (MQ=255)
                                ttAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCACTGCCAtttt                                                                                               <  2:555218/110‑1 (MQ=255)
                                         tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCACTGCCATTTTCTGCCCTTGCGAGGCCATCAGCa                                                                        <  1:481805/124‑1 (MQ=255)
                                         tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCACTGCCATTTTCTGCCCTTGCGAGGCCATCAGCa                                                                        >  2:481805/1‑124 (MQ=255)
                                         tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCACTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGa                                                         <  2:305542/139‑1 (MQ=255)
                                         tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCACTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGa                                                         >  1:305542/1‑139 (MQ=255)
                                                      gCAGACGACCGCTAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCACTGCCATTTTCTGCCCTTGCGAGGCCATCAGCACGGCAGGAATATCGACGGCCCAACCGTg                                            <  1:363980/139‑1 (MQ=255)
                                                                                         cAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCACTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTAcgcc         <  2:52688/139‑1 (MQ=255)
                                                                                                aaTCAAAAACCGGGTTGCCCTGATTATGGAACATCACTGCCATTTTCTGCCCTTGCGa                                                                                   >  1:386371/1‑58 (MQ=255)
                                                                                                aaTCAAAAACCGGGTTGCCCTGATTATGGAACATCACTGCCATTTTCTGCCCTTGCGa                                                                                   <  2:386371/58‑1 (MQ=255)
                                                                                                aaTCAAAAACCGGGTTGCCCTGATTATGGAACATCACTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCAtctc  >  1:554234/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                       
AGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTC  >  NC_000913/1939587‑1939821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A