Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	82	28.6	1225488	97.4%	1193625	116.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,913,461	T→C	E64G (GAG→GGG)	relA ←	(p)ppGpp synthetase I/GTP pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,913,461	0	T	C	100.0%	133.0 / NA	35	E64G (GAG→GGG)	relA	(p)ppGpp synthetase I/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/16); total (19/16)

CGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGAT  >  NC_000913/2913327‑2913591

cGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCccac                                                                                                                                <  2:187519/139‑1 (MQ=255)

cGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCccac                                                                                                                                <  2:385591/139‑1 (MQ=255)

        aGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCaa                                                                                                                        >  1:218698/1‑139 (MQ=255)

                   cgacTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGc                                                                                                                <  2:609923/136‑1 (MQ=255)

                   cgacTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGc                                                                                                                >  1:609923/1‑136 (MQ=255)

                                ctctCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTgg                                                                                                >  1:350904/1‑139 (MQ=255)

                                   tcACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCCCGCCACAATAACAGACTGGCAt                                                                                             <  1:115792/139‑1 (MQ=255)

                                             caTCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATACCAGACTGGCATCCGGATGccc                                                                                   >  2:360377/1‑139 (MQ=255)

                                             caTCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGAc                                                                                                   <  1:278133/123‑1 (MQ=255)

                                             caTCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGAc                                                                                                   >  2:278133/1‑123 (MQ=255)

                                                   cGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGt                                                                             <  2:569970/139‑1 (MQ=255)

                                                   cGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGt                                                                             <  1:360377/139‑1 (MQ=255)

                                                     cTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCt                                                                           >  2:370679/1‑139 (MQ=255)

                                                           aCGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCa                                                                     <  2:257686/139‑1 (MQ=255)

                                                               tGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATGCccac                                                                                                                                <  2:576276/76‑1 (MQ=255)

                                                                  cATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATa                                                              >  2:499205/1‑139 (MQ=255)

                                                                      cGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGcc                                                          <  1:370679/139‑1 (MQ=255)

                                                                                     cagcGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAg                                           <  2:218698/139‑1 (MQ=255)

                                                                                        cgccgcCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGt                                                                             <  1:115155/102‑1 (MQ=255)

                                                                                        cgccgcCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGt                                                                             >  2:115155/1‑102 (MQ=255)

                                                                                        cgccgcCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGtt                                                                        >  2:60581/1‑107 (MQ=255)

                                                                                        cgccgcCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGtt                                                                        <  1:60581/107‑1 (MQ=255)

                                                                                        cgccgcCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCAc                                        >  2:87909/1‑139 (MQ=255)

                                                                                          ccgcccgcAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTc                                      >  2:252842/1‑139 (MQ=255)

                                                                                             cccgcAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTcaca                                   >  2:26728/1‑139 (MQ=255)

                                                                                                                        aTGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGttt                                                   <  1:60065/96‑1 (MQ=255)

                                                                                                                        aTGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGttt                                                   >  2:60065/1‑96 (MQ=255)

                                                                                                                        aTGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACt                                       <  2:454316/108‑1 (MQ=255)

                                                                                                                        aTGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACt                                       >  1:454316/1‑108 (MQ=255)

                                                                                                                         tGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACtt       >  2:103467/1‑139 (MQ=255)

                                                                                                                          gTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTg      <  2:350904/139‑1 (MQ=255)

                                                                                                                          gTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAACACTCACACGACTTCTGGCTGGTAATACCCAGACTTg      >  2:606551/1‑139 (MQ=255)

                                                                                                                            cGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCg    >  1:91349/1‑139 (MQ=255)

                                                                                                                              agagGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGAt  >  1:91624/1‑139 (MQ=255)

                                                                                                                              agagGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGAt  >  1:302792/1‑139 (MQ=255)

CGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGAT  >  NC_000913/2913327‑2913591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A