Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I1 R1
|
82 |
28.6 |
1225488 |
97.4% |
1193625 |
116.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,491,277 |
T→C |
T417A (ACG→GCG) |
idnT ← |
L‑idonate and D‑gluconate transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,491,277 | 0 | T | C | 100.0%
| 90.8
/ NA
| 26 | T417A (ACG→GCG) | idnT | L‑idonate and D‑gluconate transporter |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/12); total (14/12) |
GGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGC > NC_000913/4491156‑4491406
|
cggcggatcttcaatcttcCCCCTAAACAACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTGAATGGCTAACACGACCAGCAAACCCATAATAGTTATAAGGGTTGCCATCACCGCCCACGTACGCAACAttt > 1:425188/7‑139 (MQ=255)
ttcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCg > 2:235561/1‑139 (MQ=255)
cATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTaa < 1:2963/139‑1 (MQ=255)
atatTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCa < 2:128153/139‑1 (MQ=255)
tATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACg > 2:535099/1‑88 (MQ=255)
tATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACg < 1:535099/88‑1 (MQ=255)
tATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCa < 1:106579/101‑1 (MQ=255)
tATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCa > 2:106579/1‑101 (MQ=255)
tATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGcc > 2:135293/1‑139 (MQ=255)
cTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAggg < 1:519218/139‑1 (MQ=255)
tttCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCAcc > 2:351769/1‑95 (MQ=255)
tttCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCAcc < 1:351769/95‑1 (MQ=255)
cGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGAcgc > 2:550316/1‑139 (MQ=255)
ttAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAAt < 2:441272/94‑1 (MQ=255)
ttAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAAt > 1:441272/1‑94 (MQ=255)
tAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGAt > 2:13734/1‑139 (MQ=255)
aTGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCa > 2:289032/1‑139 (MQ=255)
tAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTg > 2:499430/1‑139 (MQ=255)
acacGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAAt < 2:87118/84‑1 (MQ=255)
acacGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAAt > 1:87118/1‑84 (MQ=255)
ccAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACCCTGCCCGcccc > 2:354874/1‑139 (MQ=255)
aGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCgg < 1:289032/139‑1 (MQ=255)
cAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGtt > 1:316905/1‑139 (MQ=255)
tAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTAc < 2:463791/91‑1 (MQ=255)
tAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTAc > 1:463791/1‑91 (MQ=255)
ccATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGc < 1:550316/139‑1 (MQ=255)
ccATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCGTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGc < 1:135293/139‑1 (MQ=255)
|
GGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGC > NC_000913/4491156‑4491406
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
