Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I2 R1
|
97 |
53.3 |
2484976 |
94.5% |
2348302 |
111.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,491,277 |
T→C |
T417A (ACG→GCG) |
idnT ← |
L‑idonate and D‑gluconate transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,491,277 | 0 | T | C | 100.0%
| 169.5
/ NA
| 44 | T417A (ACG→GCG) | idnT | L‑idonate and D‑gluconate transporter |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/19); total (25/19) |
CGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGCATGG > NC_000913/4491152‑4491410
|
cGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAAc > 2:864510/1‑139 (MQ=255)
ggggAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTc > 2:229466/1‑139 (MQ=255)
tcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGt < 1:864510/139‑1 (MQ=255)
ccccGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTaaa > 2:1042139/1‑139 (MQ=255)
cccGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTaaaa < 2:1098574/139‑1 (MQ=255)
ccGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAAt < 1:229466/139‑1 (MQ=255)
caATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGc > 2:728144/1‑139 (MQ=255)
tATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGcc < 1:896081/132‑1 (MQ=255)
tATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGcc > 2:896081/1‑132 (MQ=255)
tCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCg > 2:62557/1‑139 (MQ=255)
tCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCg > 1:1073947/1‑139 (MQ=255)
gCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGAcg < 1:1031580/139‑1 (MQ=255)
cGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCa > 2:191043/1‑112 (MQ=255)
cGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCa < 1:191043/112‑1 (MQ=255)
cGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGAcgc > 2:261870/1‑139 (MQ=255)
cGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGAcgc > 2:629810/1‑139 (MQ=255)
ttAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGa > 2:422743/1‑139 (MQ=255)
ttAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGa > 2:927548/1‑139 (MQ=255)
tAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTc < 1:533525/83‑1 (MQ=255)
tAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTc > 2:533525/1‑83 (MQ=255)
acacGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGc < 2:639135/56‑1 (MQ=255)
acacGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGc > 1:639135/1‑56 (MQ=255)
acGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCg < 1:629810/139‑1 (MQ=255)
gCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGcc > 1:475441/1‑102 (MQ=255)
gCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGcc < 2:475441/102‑1 (MQ=255)
cccAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACgt < 1:693291/115‑1 (MQ=255)
cccAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACgt > 2:693291/1‑115 (MQ=255)
ccAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCAcc > 2:676065/1‑139 (MQ=255)
aaaTAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGt > 1:566434/1‑49 (MQ=255)
aaaTAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGt < 2:566434/49‑1 (MQ=255)
aaaTAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGt > 1:235813/1‑49 (MQ=255)
aaaTAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGt < 2:235813/49‑1 (MQ=255)
aaTAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAAt > 1:1179584/1‑59 (MQ=255)
aaTAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAAt < 2:1179584/59‑1 (MQ=255)
aTAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAg < 2:1073947/139‑1 (MQ=255)
aGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCgg > 1:404765/1‑139 (MQ=255)
aGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCgg > 1:902932/1‑139 (MQ=255)
ggTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGa > 2:1114455/1‑139 (MQ=255)
gTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGAt < 2:404765/139‑1 (MQ=255)
tCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGt > 2:1106527/1‑38 (MQ=255)
tCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGt > 2:568540/1‑120 (MQ=255)
tCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGt < 1:568540/120‑1 (MQ=255)
ccATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGc < 1:676065/139‑1 (MQ=255)
cACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGCATgg < 1:361475/139‑1 (MQ=255)
|
CGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGCATGG > NC_000913/4491152‑4491410
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
