Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
73 |
24.7 |
1166784 |
93.9% |
1095610 |
107.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,620,907 |
(A)8→7 |
intergenic (‑35/‑290) |
upp ← / → purM |
uracil phosphoribosyltransferase/phosphoribosylaminoimidazole synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,620,900 | 0 | A | . | 100.0%
| 100.3
/ NA
| 24 | intergenic (‑28/‑297) | upp/purM | uracil phosphoribosyltransferase/phosphoribosylaminoimidazole synthetase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (10/14); total (10/14) |
ACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTATGAAA > NC_000913/2620769‑2621017
|
aCTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACaaaaaaat > 2:492147/1‑138 (MQ=255)
gAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACgg < 1:569526/139‑1 (MQ=255)
gAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACgg < 2:279692/139‑1 (MQ=255)
gAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATtat < 1:492147/139‑1 (MQ=255)
gTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATAc < 1:481449/139‑1 (MQ=255)
atatCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAata > 1:520522/1‑139 (MQ=255)
ttGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtt > 1:249313/1‑139 (MQ=255)
cTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTc > 2:294135/1‑139 (MQ=255)
tCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGt < 1:294135/139‑1 (MQ=255)
tgtTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGt < 1:447243/129‑1 (MQ=255)
tgtTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGt > 2:447243/1‑129 (MQ=255)
tgtTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATa > 1:20188/1‑139 (MQ=255)
tgtTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATa < 2:20188/139‑1 (MQ=255)
tttGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGt > 1:121280/1‑139 (MQ=255)
ttGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAACAATTCGACGGATTATACCTCATTTCTGCTAGGCGGCACTATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGtt < 1:159250/139‑1 (MQ=255)
aCGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTatat < 2:249313/139‑1 (MQ=255)
tttCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAg < 1:512272/113‑1 (MQ=255)
tttCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAg > 2:512272/1‑113 (MQ=255)
ttCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTATGaaa < 2:121280/139‑1 (MQ=255)
tctcCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTATGaaa > 2:201899/1‑138 (MQ=255)
tctcCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTATGaaa < 1:201899/138‑1 (MQ=255)
ttGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAg < 1:79621/50‑1 (MQ=255)
ttGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAg > 2:79621/1‑50 (MQ=255)
ttGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTATGaaa < 1:516763/132‑1 (MQ=255)
ttGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTATGaaa > 2:516763/1‑132 (MQ=255)
|
ACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTATGAAA > NC_000913/2620769‑2621017
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
