Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
73 |
24.7 |
1166784 |
93.9% |
1095610 |
107.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,852,122 |
G→A |
G87S (GGC→AGC) |
hypC → |
hydrogenase maturation protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,852,122 | 0 | G | A | 100.0%
| 87.0
/ NA
| 26 | G87S (GGC→AGC) | hypC | hydrogenase maturation protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/12); total (14/12) |
CGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATGGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTGCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCGGA > NC_000913/2851990‑2852244
|
cgcgTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAgg > 2:418518/1‑139 (MQ=255)
gCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATcgc > 2:94688/1‑139 (MQ=255)
taatGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCTCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGc < 1:418518/139‑1 (MQ=255)
gCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAAt > 2:48716/1‑139 (MQ=255)
aGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGATTATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGc < 1:94688/139‑1 (MQ=255)
cgACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATcgcgcg > 1:345867/1‑105 (MQ=255)
cgACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATcgcgcg < 2:345867/105‑1 (MQ=255)
acTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGa > 2:152316/1‑139 (MQ=255)
tctcGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAAc < 1:245264/139‑1 (MQ=255)
cGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTg > 1:316762/1‑139 (MQ=255)
tACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTGCTTCACAt < 1:152316/139‑1 (MQ=255)
aCAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGc < 2:64389/115‑1 (MQ=255)
aCAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGc > 1:64389/1‑115 (MQ=255)
gTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCg < 1:386248/56‑1 (MQ=255)
gTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCg > 2:386248/1‑56 (MQ=255)
gTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTgcg < 2:527626/113‑1 (MQ=255)
gTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTgcg > 1:527626/1‑113 (MQ=255)
tttGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATcg > 1:166998/1‑73 (MQ=255)
tttGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATcg < 2:166998/73‑1 (MQ=255)
aGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTGCTTCACATCTCTCTTACACCGCCGAACGc > 1:491912/1‑139 (MQ=255)
gTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTGCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCg < 2:491912/139‑1 (MQ=255)
cGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTGCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCGGa > 1:4973/1‑139 (MQ=255)
gcTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTGCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCg < 2:446726/131‑1 (MQ=255)
gcTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTGCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCg > 1:511636/1‑131 (MQ=255)
gcTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTGCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCg < 2:511636/131‑1 (MQ=255)
gcTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTGCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCg > 1:446726/1‑131 (MQ=255)
|
CGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATGGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTGCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCGGA > NC_000913/2851990‑2852244
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
