Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
73 |
24.7 |
1166784 |
93.9% |
1095610 |
107.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,863,567 |
(T)7→8 |
intergenic (+66/‑26) |
ygbK → / → ygbL |
FliA‑regulated DUF1537 family protein/putative class II aldolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,863,560 | 1 | . | T | 100.0%
| 92.3
/ NA
| 27 | intergenic (+59/‑33) | ygbK/ygbL | FliA‑regulated DUF1537 family protein/putative class II aldolase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (14/13); total (14/13) |
CCTGGGAATAAAAGGGTTTCATATTGGCCCAACCATTTCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCC‑TTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAGCGATTTCGCAAAAGTAGAGCAGTCTTTGCGAGAGGAGATGACGCGGATTGCCAGTTCATTCTTTCAGCGCGGCTATGCAACCGGTTCGG > NC_000913/2863444‑2863686
|
ccTGGGAATAAAAGGGTTTCATATTGGCCCAACCATTTCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAaga > 2:397935/1‑139 (MQ=255)
ggAATAAAAGGGTTTCATATTGGCCCAACCATTTCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAAGAGAGt > 2:411803/1‑139 (MQ=255)
tAAAAGGGTTTCATATTGGCCCAACCATTTCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAAGAGAGttttt > 1:121448/1‑139 (MQ=255)
tttCATATTGGCCCAACCATTTCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGAAGACGCCTTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAg < 1:411803/139‑1 (MQ=255)
tCATATTGGCCCAACCATTTCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCC‑ttttttttcacg < 2:290284/111‑6 (MQ=255)
tCATATTGGCCCAACCATTTCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCC‑ttttttttcacg > 1:290284/1‑106 (MQ=255)
tGGCCCAACCATTTCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTTGCGACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAGCGATTTCg > 2:518162/1‑139 (MQ=255)
gCCCAACCATTTCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCC‑ttttttttcacg > 2:382361/1‑98 (MQ=255)
gCCCAACCATTTCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCAGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCC‑ttttttttcacg < 1:382361/103‑6 (MQ=255)
tttCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAGCGATTTCGCAAAAGTagag < 1:397935/139‑1 (MQ=255)
gCGTGCCGTGGGTAAACGCACTGGATAATCCTGTCTCACTCGCCCTTAACTCTGGCAACTTAGGTGATGACGCCTTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAGCGATTTCGCAAAAGTAGAGCAGTCttt > 1:375129/1‑139 (MQ=255)
tGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAGCGATTTCGCAAAAGTAGAGCAGTCTTTGCg < 1:242451/139‑1 (MQ=255)
cGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAGCGATTTCGCAAAAGTAGAGCAGTCTTTGCgaga < 2:121448/139‑1 (MQ=255)
cTGGATAAGCCTGTCTCACTCGCCCTTAAATCT‑GCAACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAGCGATTTCGCAAAAGTagag > 1:287178/1‑110 (MQ=255)
cTGGATAAGCCTGTCTCACTCGCCCTTAAATCT‑GCAACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAGCGATTTCGCAAAAGTagag < 2:287178/110‑1 (MQ=255)
tctcACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAagag > 1:39241/1‑64 (MQ=255)
tctcACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAagag < 2:39241/64‑1 (MQ=255)
tctcACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAag > 1:360463/1‑62 (MQ=255)
tctcACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAag < 2:360463/62‑1 (MQ=255)
tctcACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAGCGATTTCGCAAAAGTAGAGCAGTCTTTGCGAGAGGAGATGACGCGGATTGCCAGTTCAtt > 1:308998/1‑139 (MQ=255)
tCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAGCGATTTCGCAAAAGTAGAGCAGTCTTTGCGAGAGGAGATGACGCGGATTGCCAGTTCATTCTTTCa > 1:511426/1‑139 (MQ=255)
cGCACTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAGCGATTTCGCAAAAGTAGAGCAGTCTTTGCGAGAGGAGATTACGCGGATTGCCAGTTCATTCTTTCAg < 2:375129/139‑1 (MQ=255)
ggCAACTTCGGTGATGACGCCTTTTTTTTCACGAGc < 2:195938/36‑1 (MQ=37)
ggCAACTTCGGTGATGACGCCTTTTTTTTCACGAGc > 1:195938/1‑36 (MQ=37)
ggCAACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAGCGATTTCGCaa < 1:450400/70‑1 (MQ=255)
ggCAACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAGCGATTTCGCaa > 2:450400/1‑70 (MQ=255)
ggCAACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAGCGATTTCGCAAAAGTAGAGCa < 2:24827/80‑1 (MQ=255)
ggCAACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAGCGATTTCGCAAAAGTAGAGCa > 1:24827/1‑80 (MQ=255)
ggCAACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAGCGATTTCGCAAAAGTAGAGCAGTCTTTGCGAGAGGAGATGACGCGGATTGCCAGTTCATTCTTTCAg > 1:431562/1‑126 (MQ=255)
ggCAACTTCGGTGATGACGCCTTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAGCGATTTCGCAAAAGTAGAGCAGTCTTTGCGAGAGGAGATGACGCGGATTGCCAGTTCATTCTTTCAg < 2:431562/126‑1 (MQ=255)
ggTGATGACGCCTTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAGCGATTTCGCAAAAGTAGAGCAGTCTTTGCGAGAGGAGATGACGCGGATTGCCAGTTCATTCTTTCAGCGCGGCTATGCAACCGGTTCgg < 2:308998/139‑1 (MQ=255)
|
CCTGGGAATAAAAGGGTTTCATATTGGCCCAACCATTTCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCC‑TTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAGCGATTTCGCAAAAGTAGAGCAGTCTTTGCGAGAGGAGATGACGCGGATTGCCAGTTCATTCTTTCAGCGCGGCTATGCAACCGGTTCGG > NC_000913/2863444‑2863686
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
