Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
73 |
24.7 |
1166784 |
93.9% |
1095610 |
107.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,530,091 |
G→A |
T106T (ACG→ACA) |
hslO → |
heat shock protein Hsp33 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,530,091 | 0 | G | A | 100.0%
| 96.9
/ NA
| 29 | T106T (ACG→ACA) | hslO | heat shock protein Hsp33 |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/16); total (13/16) |
ATATCACCGTACAGCTGCAGGGCGACGGTCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGA > NC_000913/3529964‑3530198
|
atatCACCGTACAGCTGCAGGGCGACGGTCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCaa < 2:206677/139‑1 (MQ=255)
tACAGCTGCAGGGCGACGGTCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCgg > 1:237455/1‑127 (MQ=255)
tACAGCTGCAGGGCGACGGTCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCgg < 2:237455/127‑1 (MQ=255)
cTGCGGGGCGACGGTCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGa > 2:426455/1‑139 (MQ=255)
cTGCAGGGCGACGGTCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGa > 2:490900/1‑139 (MQ=255)
tGCAGGGCGACGGTCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGAt < 1:136479/139‑1 (MQ=255)
tGCAGGGCGACGGTCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGAt < 1:329994/139‑1 (MQ=255)
ggCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGaa < 2:300156/139‑1 (MQ=255)
tAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCa < 2:445435/104‑1 (MQ=255)
tAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCa > 1:445435/1‑104 (MQ=255)
aaCAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACg > 1:354941/1‑91 (MQ=255)
aaCAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACg < 2:354941/91‑1 (MQ=255)
aaCCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTc < 1:426455/139‑1 (MQ=255)
cagATGCGCGGTGTGGCGCGCGTGCAGGGCTAAATTCCAGAAAATGCCGACCTGAAAACAGTGGTCGGCAATGGTTACGTGGTGAt < 1:538987/86‑1 (MQ=255)
cagATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTAGGCAATGGTTACGTGGTGAt > 2:538987/1‑86 (MQ=255)
agATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGAt < 1:114921/85‑1 (MQ=255)
agATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGAt > 2:114921/1‑85 (MQ=255)
agATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCg > 2:27421/1‑102 (MQ=255)
agATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCg < 1:27421/102‑1 (MQ=255)
aTGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTg > 1:329141/1‑139 (MQ=255)
cgGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCg < 1:443896/95‑1 (MQ=255)
cgGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCg > 2:443896/1‑95 (MQ=255)
gTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTggcggc < 2:335212/134‑1 (MQ=255)
gTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTggcggc > 1:335212/1‑134 (MQ=255)
gTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGgcctgcc < 2:329141/139‑1 (MQ=255)
gTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGAGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGgcctgcc > 1:375002/1‑139 (MQ=255)
ggCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTA‑CCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGa < 1:490900/139‑1 (MQ=255)
cGAAATTCCAGAAAATGCCGACCTGAAAACACTGGt > 1:434917/1‑36 (MQ=255)
cGAAATTCCAGAAAATGCCGACCTGAAAACACTGGt < 2:434917/36‑1 (MQ=255)
|
ATATCACCGTACAGCTGCAGGGCGACGGTCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGA > NC_000913/3529964‑3530198
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
