Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
73 |
24.7 |
1166784 |
93.9% |
1095610 |
107.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,950,468 |
(G)5→4 |
intergenic (+48/‑39) |
ilvL → / → ilvX |
ilvG operon leader peptide/uncharacterized protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,950,464 | 0 | G | . | 100.0%
| 96.3
/ NA
| 23 | intergenic (+44/‑43) | ilvL/ilvX | ilvG operon leader peptide/uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (12/11); total (12/11) |
GATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGC > NC_000913/3950342‑3950588
|
gATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCt > 1:163410/1‑139 (MQ=255)
aTTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCtt > 2:505467/1‑139 (MQ=255)
tGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCTTAAAAACa < 1:433719/139‑1 (MQ=255)
ggTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCTTAAAAACAt > 1:474874/1‑139 (MQ=255)
gTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCTTAAAAACa > 1:566320/1‑137 (MQ=255)
gTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCTTAAAAACa < 2:566320/137‑1 (MQ=255)
gCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGc > 2:286124/1‑139 (MQ=255)
cGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa < 1:505467/139‑1 (MQ=255)
cGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa < 1:107967/116‑1 (MQ=255)
cGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa > 2:107967/1‑116 (MQ=255)
gTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCtt > 1:237906/1‑95 (MQ=255)
gTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCtt < 2:237906/95‑1 (MQ=255)
ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACg < 1:309414/139‑1 (MQ=255)
aaGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAAt > 1:432915/1‑139 (MQ=255)
aaGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAAt > 1:41640/1‑139 (MQ=255)
ttAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGgcgc < 2:41640/139‑1 (MQ=255)
aaCGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCTTAAAAACATAACCgaggag < 1:222140/67‑1 (MQ=255)
aaCGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCTTAAAAACATAACCgaggag > 2:222140/1‑67 (MQ=255)
gACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACg < 1:138535/105‑1 (MQ=255)
gACCCCCGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACg > 2:138535/1‑105 (MQ=255)
cGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa > 2:514257/1‑55 (MQ=255)
cGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa < 1:514257/55‑1 (MQ=255)
cGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGc < 2:474874/139‑1 (MQ=255)
|
GATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGC > NC_000913/3950342‑3950588
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
