Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I1 R1	73	24.7	1166784	93.9%	1095610	107.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,077,129	G→A	E39K (GAA→AAA)	dtd →	D‑tyr‑tRNA(Tyr) deacylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,077,129	0	G	A	95.7%	69.1 / ‑3.4	23	E39K (GAA→AAA)	dtd	D‑tyr‑tRNA(Tyr) deacylase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (14/8); total (14/9)
Fisher's exact test for biased strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

ACGAACCATGATTGCATTAATTCAACGCGTAACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTC  >  NC_000913/4077008‑4077257

acgaACCATGATTGCATTAATTCAACGCGTAACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGt                                                                                                                 <  1:161047/139‑1 (MQ=255)

 cgaACCATGATTGCATTAATTCAACGCGTAACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACAAACAGAAAGCAAACCGTc                                                                                                                >  1:391588/1‑139 (MQ=255)

           ttGCATTAATTCAACGCGTAACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACAAACAGAAAGCAAACCGTCTGTGCGAGCg                                                                                                      >  2:430191/1‑139 (MQ=255)

              cATTAATTCAACGCGTAACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACAAACAGAAAGCAAACCGTCTGTGCGAGCgtgt                                                                                                   >  1:409077/1‑139 (MQ=255)

                       aaCGCGTAACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACAAAc                                                                                                                               >  2:548004/1‑102 (MQ=255)

                       aaCGCGTAACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACAAAc                                                                                                                               <  1:548004/102‑1 (MQ=255)

                              aaCCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACAAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATc                                                                                   >  1:107723/1‑139 (MQ=255)

                                                        gagaAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACAAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTAcc                                                                                        >  1:556490/1‑108 (MQ=255)

                                                        gagaAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACAAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTAcc                                                                                        <  2:556490/108‑1 (MQ=255)

                                                          gaAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACAAACAGAAAGCAAACCGTCTGTGCGAGc                                                                                                       >  1:277686/1‑91 (MQ=255)

                                                          gaAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACAAACAGAAAGCAAACCGTCTGTGCGAGc                                                                                                       <  2:277686/91‑1 (MQ=255)

                                                            aGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACAAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAAtctc                                                     >  2:200816/1‑139 (MQ=255)

                                                                 cGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACAAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCa                                                                <  1:467893/123‑1 (MQ=255)

                                                                 cGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACAAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCa                                                                >  2:467893/1‑123 (MQ=255)

                                                                 cGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACAAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGt                                                >  1:223097/1‑139 (MQ=255)

                                                                                                aTTGGGTGTCGAAAAGGATGACGACAAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGtt                 >  1:65241/1‑139 (MQ=255)

                                                                                                aTTGGGTGTCGAAAAGGATGACGACAAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGtt                 >  2:103966/1‑139 (MQ=255)

                                                                                                 ttGGGTGTCGAAAAGGATGACGACAAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGttt                <  1:430191/139‑1 (MQ=255)

                                                                                                        tCGAAAAGGATGACGACAAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCaa                                                   <  1:146042/97‑1 (MQ=255)

                                                                                                        tCGAAAAGGATGACGACAAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCaa                                                   >  2:146042/1‑97 (MQ=255)

                                                                                                           aaaaGGATGACGACAAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCt                                                                                                <  2:219870/49‑1 (MQ=255)

                                                                                                           aaaaGGATGACGACAAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCt                                                                                                >  1:219870/1‑49 (MQ=255)

                                                                                                               ggATGACGACAAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTc  <  2:65241/139‑1 (MQ=255)

ACGAACCATGATTGCATTAATTCAACGCGTAACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTC  >  NC_000913/4077008‑4077257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A