Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	66	20.9	912876	97.7%	891879	114.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,078,169	C→T	R241C (CGT→TGT)	yiiD →	GNAT family putative N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,078,169	0	C	T	100.0%	70.1 / NA	21	R241C (CGT→TGT)	yiiD	GNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (12/9); total (12/9)

ACCGGGCAAAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGCGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCTCGGTGCCTTAAGCGGCGATCTCGACCGTCTGGCGCGCGG  >  NC_000913/4078043‑4078302

aCCGGGCAAAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCAcc                                                                                                                           >  1:208559/1‑139 (MQ=255)

    ggCAAAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTcgg                                                                                                                       >  1:402854/1‑139 (MQ=255)

                                 aCCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCata                                                                                          <  2:61955/139‑1 (MQ=255)

                                    ggCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATcc                                                                                       >  2:83286/1‑139 (MQ=255)

                                     gCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCg                                                                                      <  2:208559/139‑1 (MQ=255)

                                                    aCCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAAc                                                                                                                 >  1:225003/1‑97 (MQ=255)

                                                    aCCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAAc                                                                                                                 <  2:225003/97‑1 (MQ=255)

                                                       aTACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAg                                                                    >  2:132140/1‑139 (MQ=255)

                                                                    cGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATg                                                                                                >  2:113525/1‑98 (MQ=255)

                                                                    cGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATg                                                                                                <  1:113525/98‑1 (MQ=255)

                                                                       gAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGc                                                                               <  2:42755/112‑1 (MQ=255)

                                                                       gAGTTTATTCTCACTGGCGACGCTCACTGGTTAGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGc                                                                               >  1:42755/1‑112 (MQ=255)

                                                                         gTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCg                                                    >  1:442797/1‑137 (MQ=255)

                                                                         gTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCg                                                    <  2:442797/137‑1 (MQ=255)

                                                                         gTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGt                                                  >  2:339658/1‑139 (MQ=255)

                                                                           ttATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAg                                                <  2:259492/139‑1 (MQ=255)

                                                                                  cACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCACATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCt                                         >  1:162162/1‑139 (MQ=255)

                                                                                   aCTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCTc                                        >  2:226102/1‑139 (MQ=255)

                                                                                                       ggggACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCTCGGTGCCTTAAgcgg                          >  1:394422/1‑133 (MQ=255)

                                                                                                       ggggACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCTCGGTGCCTTAAgcgg                          <  2:394422/133‑1 (MQ=255)

                                                                                                         ggACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATTCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCTCGGTGCCTTAAGCGGCGATCTCg                  <  1:362295/139‑1 (MQ=255)

                                                                                                                         tgctgtGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCTCGGTGCCTTAAGCGGCGATCTCGACCGTCTGGCGCGCgg  >  2:126828/1‑139 (MQ=255)

ACCGGGCAAAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGCGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCTCGGTGCCTTAAGCGGCGATCTCGACCGTCTGGCGCGCGG  >  NC_000913/4078043‑4078302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A