Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
65 |
21.2 |
1002576 |
96.5% |
967485 |
111.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,078,169 |
C→T |
R241C (CGT→TGT) |
yiiD → |
GNAT family putative N‑acetyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,078,169 | 0 | C | T | 100.0%
| 40.2
/ NA
| 13 | R241C (CGT→TGT) | yiiD | GNAT family putative N‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (8/5); total (8/5) |
GGCAAAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGCGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCTCGGTGCCTTAAGCGGCGATCTCGACCGTCTGGCGC > NC_000913/4078047‑4078298
|
ggCAAAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTcgg > 1:344507/1‑139 (MQ=255)
ggCAAAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTcgg > 2:229601/1‑139 (MQ=255)
aaaaaTTTATCACTACCATTCGAGAAACCGGCAATCAGAAACCGCACCATACGCTGTTTGCCTGGAGTTGATTCTCACTGGCGACGGGCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTcggcgg < 2:356841/139‑1 (MQ=255)
tttATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGa < 1:229601/139‑1 (MQ=255)
gCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCg > 1:76204/1‑139 (MQ=255)
cATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTa < 2:76204/139‑1 (MQ=255)
tATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCata > 1:287471/1‑96 (MQ=255)
tATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCata < 2:287471/96‑1 (MQ=255)
cTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCTCGGTGCCTTAAgcgg > 2:233574/1‑139 (MQ=255)
gACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCTCGGTGCCTTAAGCGGCGATCTCGa > 1:421927/1‑139 (MQ=255)
aTCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCgg < 2:309570/52‑1 (MQ=255)
aTCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATAATTCTTGCgc > 1:309570/1‑51 (MQ=255)
cTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCTCGGTGCCTTAAGCGGCGATCTCGACCGTCTGgcgc > 2:483433/1‑139 (MQ=255)
tgctgtGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCTCGGTGCCTTAAgcg < 1:414309/114‑1 (MQ=255)
tgctgtGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCTCGGTGCCTTAAgcg > 2:414309/1‑114 (MQ=255)
|
GGCAAAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGCGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCTCGGTGCCTTAAGCGGCGATCTCGACCGTCTGGCGC > NC_000913/4078047‑4078298
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
