Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I1 R1 81 28.9 1316934 95.8% 1261622 113.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,934,968 T→C V449V (GTA→GTG)  zwf ← glucose‑6‑phosphate 1‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,934,9680TC100.0% 60.9 / NA 17V449V (GTA→GTG) zwfglucose‑6‑phosphate 1‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/10);  total (7/10)

GATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATC  >  NC_000913/1934835‑1935102
                                                                                                                                     |                                                                                                                                      
gATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCCACCCa                                                                                                                                   >  2:640241/1‑139 (MQ=255)
                  tCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCCACCCATTTCCAGGCTTCTTCCAc                                                                                                                 >  2:446220/1‑139 (MQ=255)
                  tCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCCACCCATTTCCAGGCTTCTTCCAc                                                                                                                 >  2:171808/1‑139 (MQ=255)
                          cGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCCACCCATTTCCAGGCTTCTTCCACTTCGTcgc                                                                                                         <  1:171808/139‑1 (MQ=255)
                          cGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCCACCCATTTCCAGGCTTCTTCCACTTCGTcgc                                                                                                         <  1:446220/139‑1 (MQ=255)
                            aCCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATTCGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCCACCCATTTCCAGGCTTCTTCCACTTCGTCGCGa                                                                                                       <  1:224075/139‑1 (MQ=255)
                                       tAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCCACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAg                                                                                            >  2:103416/1‑139 (MQ=255)
                                                        gCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCCACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCa                                                                           <  1:640241/139‑1 (MQ=255)
                                                        gCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCCACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCa                                                                           <  2:71962/139‑1 (MQ=255)
                                                                        ttCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCCACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCaa                                                           <  1:143802/139‑1 (MQ=255)
                                                                         tCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCCACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCaaa                                                          >  2:188236/1‑139 (MQ=255)
                                                                          ccGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCCACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGc                                                                                         >  1:542305/1‑107 (MQ=255)
                                                                          ccGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCCACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGc                                                                                         <  2:542305/107‑1 (MQ=255)
                                                                                     cGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCCACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCa                                              <  2:70204/139‑1 (MQ=255)
                                                                                             gcgcATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCCACCCAt                                                                                                                                  >  2:421552/1‑47 (MQ=255)
                                                                                             gcgcATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCCACCCAt                                                                                                                                  <  1:421552/47‑1 (MQ=255)
                                                                                                                                 aGTCCACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATc  <  1:458430/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                      
GATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATC  >  NC_000913/1934835‑1935102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A