Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I1 R1	81	28.9	1316934	95.8%	1261622	113.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,097,854	G→A	L196L (CTG→CTA)	yggW →	HemN family putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,097,854	0	G	A	100.0%	108.7 / NA	32	L196L (CTG→CTA)	yggW	HemN family putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/14); total (18/14)

GATTTGATGCATGGGCTGCCGGATCAATCACTGGAAGAGGCGCTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCT  >  NC_000913/3097753‑3097986

gatttgatGCATGGGCTGCCGGATCAATCACTGGAAGAGGCGCTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGaccac                                                                                                 >  1:383779/1‑139 (MQ=255)

           tGGGCTGCCGGATCAATCACTGGAAGAGGCGCTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCg                                                                                      >  2:47343/1‑139 (MQ=255)

                   cGGATCAATCACTGGAAGAGGCGCTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATa                                                                                                                      <  2:167101/99‑1 (MQ=255)

                   cGGATCAATCACTGGAAGAGGCGCTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATa                                                                                                                      >  1:167101/1‑99 (MQ=255)

                   cGGATCAATCACTGGAAGAGGCGCTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGtg                                                                                            <  2:372012/125‑1 (MQ=255)

                   cGGATCAATCACTGGAAGAGGCGCTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGtg                                                                                            >  1:372012/1‑125 (MQ=255)

                               tGGAAGAGGCGCTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGa                                                                  <  1:47343/139‑1 (MQ=255)

                                 gAAGAGGCGCTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAAC‑AACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGatat                                                               >  1:367522/1‑139 (MQ=255)

                                         gcTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGaa                                                        >  1:574029/1‑139 (MQ=255)

                                         gcTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGaa                                                        >  2:446420/1‑139 (MQ=255)

                                                  tCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCAt                                               <  1:32928/139‑1 (MQ=255)

                                                     aCGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAg                                            <  2:574029/139‑1 (MQ=255)

                                                            gCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGt                                                                                             <  2:154148/83‑1 (MQ=255)

                                                            gCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGAc                                                                             <  1:170302/99‑1 (MQ=255)

                                                            gCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGAc                                                                             >  2:170302/1‑99 (MQ=255)

                                                            gCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATAtt                                                            <  1:612595/116‑1 (MQ=255)

                                                            gCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATAtt                                                            >  2:612595/1‑116 (MQ=255)

                                                            gCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTaa                                     >  1:638346/1‑139 (MQ=255)

                                                            gCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTaa                                     >  1:557485/1‑139 (MQ=255)

                                                            gCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCCGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTaa                                     >  2:568032/1‑139 (MQ=255)

                                                            gCCACTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGt                                                                                             >  1:154148/1‑83 (MQ=255)

                                                                      tGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCggg                           >  1:42293/1‑139 (MQ=255)

                                                                      tGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCggg                           <  2:557485/139‑1 (MQ=255)

                                                                           ccgccgCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCGGGTTATc                      <  1:446420/139‑1 (MQ=255)

                                                                             gccgcATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCGGGTTATCAg                    <  2:42293/139‑1 (MQ=255)

                                                                               cgcATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCGGGTTATCAGCa                  >  1:146399/1‑139 (MQ=255)

                                                                                              aTCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGAcgcg                                                                          <  1:56820/68‑1 (MQ=255)

                                                                                              aTCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGAcgcg                                                                          >  2:56820/1‑68 (MQ=255)

                                                                                               tCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCt  >  1:535886/1‑139 (MQ=255)

                                                                                               tCAACTAACCATCGAACCCAATACGCCGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCt  <  1:568032/139‑1 (MQ=255)

                                                                                                 aaCTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCAt                                               <  1:560339/92‑1 (MQ=255)

                                                                                                 aaCTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCAt                                               >  2:560339/1‑92 (MQ=255)

GATTTGATGCATGGGCTGCCGGATCAATCACTGGAAGAGGCGCTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCT  >  NC_000913/3097753‑3097986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A