Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
81 |
28.9 |
1316934 |
95.8% |
1261622 |
113.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,347,929 |
T→C |
V272A (GTC→GCC) |
yhbJ → |
adaptor protein for GlmZ/GlmY sRNA decay, glucosamine‑6‑phosphate‑regulated, NTPase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,347,929 | 0 | T | C | 100.0%
| 104.8
/ NA
| 28 | V272A (GTC→GCC) | yhbJ | adaptor protein for GlmZ/GlmY sRNA decay, glucosamine‑6‑phosphate‑regulated, NTPase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/11); total (17/11) |
CTATGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCA > NC_000913/3347796‑3348048
|
cTATGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGt > 1:140479/1‑139 (MQ=255)
ttGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGt > 1:430717/1‑139 (MQ=255)
ttGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGt > 1:365230/1‑139 (MQ=255)
gTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCa > 1:178727/1‑139 (MQ=255)
ggTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGa < 1:203405/139‑1 (MQ=255)
aCCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATcac > 2:396339/1‑139 (MQ=255)
ggcggGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaaa < 2:430717/139‑1 (MQ=255)
ggcggGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaaa < 2:365230/139‑1 (MQ=255)
gtgtATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAATCTGGGCatgcatgc < 2:140479/139‑1 (MQ=255)
tgtATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGcc < 2:178727/139‑1 (MQ=255)
atTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGcc > 2:240391/1‑139 (MQ=255)
agagCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGc > 2:327138/1‑62 (MQ=255)
agagCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGc < 1:327138/62‑1 (MQ=255)
agagCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCaa > 2:295839/1‑139 (MQ=255)
agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACg < 1:215330/49‑1 (MQ=255)
agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACg > 2:215330/1‑49 (MQ=255)
agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATg > 1:202214/1‑139 (MQ=255)
agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATg > 1:104769/1‑139 (MQ=255)
agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATg > 2:242881/1‑139 (MQ=255)
cAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACCAACAAGCTGGGCATGCATGCCCGGCCTGCAATGaa > 2:151095/1‑139 (MQ=255)
cAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGaa > 2:314854/1‑139 (MQ=255)
aaCTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAg < 1:295839/139‑1 (MQ=255)
aGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTg > 1:307974/1‑139 (MQ=255)
actTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTa > 1:90723/1‑139 (MQ=255)
cGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCa < 1:240391/139‑1 (MQ=255)
cTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAg < 1:578363/90‑1 (MQ=255)
cTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAg > 2:578363/1‑90 (MQ=255)
tCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGaa > 1:647906/1‑128 (MQ=255)
tCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGaa < 2:647906/128‑1 (MQ=255)
|
CTATGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCA > NC_000913/3347796‑3348048
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
