Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I1 R1	81	28.9	1316934	95.8%	1261622	113.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,450,674	G→A	P231S (CCA→TCA)	rplB ←	50S ribosomal subunit protein L2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,450,674	0	G	A	100.0%	76.5 / NA	23	P231S (CCA→TCA)	rplB	50S ribosomal subunit protein L2
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/8); total (15/8)

GCTACGGCGACGTACGATGAATTTATCAGTACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGGGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTCTG  >  NC_000913/3450549‑3450808

gCTACGGCGACGTACGATGAATTTATCAGTACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCggg                                                                                                                           <  1:346257/139‑1 (MQ=255)

                             tACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACg                                                                                              >  1:65346/1‑139 (MQ=255)

                              aCGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGa                                                                                             >  2:553337/1‑139 (MQ=255)

                               cGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGaa                                                                                            >  2:297774/1‑139 (MQ=255)

                                    gttgCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAAcacca                                                                                       >  2:580959/1‑139 (MQ=255)

                                           cGGGTCTTCTTACCTTTGGTCTGAATGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACg                                                                                     <  1:222252/134‑1 (MQ=255)

                                           cGGGTCTTCTTACCTTTGGTCTGAATGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACg                                                                                     >  2:222252/1‑134 (MQ=255)

                                                            ggTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAAcacca                                                                                       <  1:203215/115‑1 (MQ=255)

                                                            ggTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAAcacca                                                                                       >  2:203215/1‑115 (MQ=255)

                                                             gTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTg                                                                             <  1:611716/124‑1 (MQ=255)

                                                             gTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTg                                                                             >  2:611716/1‑124 (MQ=255)

                                                                       cccACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAAcgcg                                                    >  1:576777/1‑139 (MQ=255)

                                                                       cccACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAAcgcg                                                    >  2:157860/1‑139 (MQ=255)

                                                                           cGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCgcagc                                                <  1:580959/139‑1 (MQ=255)

                                                                                 tACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGCACgcgc                                                   >  2:459011/1‑130 (MQ=255)

                                                                                 tACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACgcgc                                                   <  1:459011/130‑1 (MQ=255)

                                                                                 tACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGc                                          >  1:42778/1‑139 (MQ=255)

                                                                                 tACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCAGCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGc                                          >  1:146063/1‑139 (MQ=255)

                                                                                                                    caccaTGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCa                                       <  2:280098/107‑1 (MQ=255)

                                                                                                                    caccaTGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCa                                       >  1:280098/1‑107 (MQ=255)

                                                                                                                         tgtgAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAAcgcg                                                    <  2:222950/89‑1 (MQ=255)

                                                                                                                         tgtgAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAAcgcg                                                    >  1:222950/1‑89 (MQ=255)

                                                                                                                         tgtgAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTCTg  >  2:445178/1‑139 (MQ=255)

GCTACGGCGACGTACGATGAATTTATCAGTACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGGGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTCTG  >  NC_000913/3450549‑3450808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A