Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
81 |
28.9 |
1316934 |
95.8% |
1261622 |
113.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,901,992 |
C→T |
A77T (GCC→ACC) |
bglH ← |
carbohydrate‑specific outer membrane porin, cryptic |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,901,992 | 0 | C | T | 100.0%
| 80.6
/ NA
| 24 | A77T (GCC→ACC) | bglH | carbohydrate‑specific outer membrane porin, cryptic |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (13/11); total (13/11) |
ATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGTTTCTCTTAAC > NC_000913/3901858‑3902101
|
aTCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTGCTGGTCGGGAACGGATTGGTGGCa < 1:370057/139‑1 (MQ=255)
aTCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCa < 2:533297/139‑1 (MQ=255)
caAATTTACTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTg < 2:429632/139‑1 (MQ=255)
aaTCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACt < 2:97079/139‑1 (MQ=255)
tCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTa < 1:567252/139‑1 (MQ=255)
tCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCt > 1:348925/1‑139 (MQ=255)
tCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGAtt > 2:40799/1‑139 (MQ=255)
aTAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCt > 2:311765/1‑139 (MQ=255)
aTCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCg < 2:205501/139‑1 (MQ=255)
ctcACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTttc < 2:348925/139‑1 (MQ=255)
aCCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGtt > 1:341388/1‑139 (MQ=255)
tACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCtt > 1:79829/1‑139 (MQ=255)
cATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGt > 1:578047/1‑139 (MQ=255)
tCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGttt < 2:612855/139‑1 (MQ=255)
ggTTTTGCGGCACTACTGGTCGGGAACGGATTggtggc < 1:20741/38‑1 (MQ=255)
ggTTTTGCGGCACTACTGGTCGGGAACGGATTggtggc > 2:20741/1‑38 (MQ=255)
ggTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGTTtctc > 1:263101/1‑139 (MQ=255)
ggTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGTTtctc > 1:430047/1‑139 (MQ=255)
ggTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGTTtctc > 1:551367/1‑139 (MQ=255)
ggTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGTTtctc > 2:558095/1‑139 (MQ=255)
tGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGTTTCTCTTAAc < 2:341388/139‑1 (MQ=255)
tGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGTTTCTCTTAAc > 1:396074/1‑139 (MQ=255)
ctGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCg < 2:523180/40‑1 (MQ=255)
ctGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCg > 1:523180/1‑40 (MQ=255)
gATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCg > 1:31596/1‑51 (MQ=255)
gATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCg < 2:31596/51‑1 (MQ=255)
|
ATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGTTTCTCTTAAC > NC_000913/3901858‑3902101
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
