Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
81 |
28.9 |
1316934 |
95.8% |
1261622 |
113.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,918,745 |
A→G |
G371G (GGT→GGC) |
atpA ← |
F1 sector of membrane‑bound ATP synthase, alpha subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,918,745 | 0 | A | G | 100.0%
| 86.4
/ NA
| 24 | G371G (GGT→GGC) | atpA | F1 sector of membrane‑bound ATP synthase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/11); total (13/11) |
GCAAACTGAGAGAACGCTGCCAGTTCACGATACTGTGCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATA > NC_000913/3918629‑3918871
|
gCAAACTGAGAGAACGCTGCCAGTTCACGATACTGTGCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGaa < 1:68413/139‑1 (MQ=255)
aaCTGAGAGAACGCTGCCAGTTCACGATACTGTGCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATAc < 1:378143/139‑1 (MQ=255)
agagaACGCTGCCAGTTCACGATACTGTGCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCg < 2:480438/139‑1 (MQ=255)
cTGCCAGTTCACGATACTGTGCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAg > 1:649361/1‑139 (MQ=255)
tgtgCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAg < 1:201390/134‑1 (MQ=255)
tgtgCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAg > 2:201390/1‑134 (MQ=255)
tgCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATc > 1:272678/1‑139 (MQ=255)
tgCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTCTTCTGTGCTGCACCACCAACCCGGGATACGGACATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATc > 1:132945/1‑139 (MQ=255)
tagCGGTACGGATACCACCGGACAGCCCTTTCATGAGCTTTGTCTGTCATGCACCCGCAACACGGGATACGGAAATGCCCGCGTTGACCGCAGGACGAATACCGGCGTCGTGCAGGTTGGTTTCCAGGAAGATCTAAcc > 1:318171/2‑139 (MQ=255)
gagCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGc > 1:117628/1‑91 (MQ=255)
gagCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGc < 2:117628/91‑1 (MQ=255)
gagCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGAcc > 2:195762/1‑139 (MQ=255)
ggATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAAt < 1:494093/139‑1 (MQ=255)
aTACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCg < 2:356265/96‑1 (MQ=255)
aTACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCg > 1:356265/1‑96 (MQ=255)
accGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCAt > 2:9032/1‑125 (MQ=255)
accGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCAt < 1:9032/125‑1 (MQ=255)
accGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGAtt > 2:498003/1‑139 (MQ=255)
tGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGgaacgaa < 2:649361/139‑1 (MQ=255)
tgctgcACCACCAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCa > 1:654890/1‑139 (MQ=255)
accaccAACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGcc < 1:524984/139‑1 (MQ=255)
ccaACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCAt < 2:502936/85‑1 (MQ=255)
ccaACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCAt > 1:502936/1‑85 (MQ=255)
ccaACACGGGATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGGTTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAg > 1:504224/1‑139 (MQ=255)
ggATACGGAAATGCCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATa > 1:402084/1‑139 (MQ=255)
|
GCAAACTGAGAGAACGCTGCCAGTTCACGATACTGTGCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATA > NC_000913/3918629‑3918871
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
