Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I51 R1 223 69.9 1534784 95.1% 1459579 214.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,012,707 A→C F37C (TTT→TGT)  metR ← methionine biosynthesis regulon transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,012,7070AC100.0% 105.4 / NA 32F37C (TTT→TGT) metRmethionine biosynthesis regulon transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (6/26);  total (6/26)

GTTCTTATGGAAATTTTCTAACGCGGGTGTCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTAAACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGAATATACAGTAACCGCCAATTATGGATGTGTAAACATCTGGACGGCTAAAATCCTTCGTCTTTTA  >  NC_000913/4012475‑4012921
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                      
gTTCTTATGGAAATTTTCTAACGCGGGTGTCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTacac                                                                                                                                                                                                                      <  2:470165/235‑1 (MQ=255)
                 cTAACGCGGGTGTCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGAt                                                                                                                                                                                                    <  1:7670/236‑1 (MQ=255)
                                     tGAATTCAGCTATGGCACTAAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAAc                                                                                                                                                                                     <  1:195458/231‑1 (MQ=255)
                                     tGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGGTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGt                                                                                                                                                                                   <  1:567432/233‑1 (MQ=255)
                                                 tGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCCGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCCCTCATAGCCGGAAGCCCAGGCGTTGTTCCAGCTCGCTTCACTGGTCGGACCGGGCGGATTGCGTCTGGTGCAAGGTCGCCGAAGCGGCTgg                                                                                                                                                                    >  2:152371/1‑235 (MQ=255)
                                                  ggCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTGGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCt                                                                                                                                                                      <  1:152371/233‑1 (MQ=255)
                                                           tGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCg                                                                                                                                                          <  1:766421/236‑1 (MQ=255)
                                                                    gcAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGc                                                                                                                                                 <  1:170616/236‑1 (MQ=255)
                                                                       gACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCaa                                                                                                                                               <  2:557791/235‑1 (MQ=255)
                                                                       gACGCGTCTGCTGCGGTTAATTGCAGGCTTGCAGGTCCGGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCaa                                                                                                                                               <  2:195504/235‑1 (MQ=255)
                                                                                         catgtcAGGCTTGCAGGGCCTGGTTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAg                                                                                                                             <  2:195836/230‑1 (MQ=255)
                                                                                         cATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAg                                                                                                                             <  1:337164/235‑1 (MQ=255)
                                                                                                     gCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACt                                                                                                                   <  1:681710/233‑1 (MQ=255)
                                                                                                            cTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTacac                                                                                                                                                                                                                      <  2:175417/127‑1 (MQ=255)
                                                                                                                   attgggagcggTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTcc                                                                                                   <  2:287285/225‑1 (MQ=255)
                                                                                                                                         ccAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTg                                                                            <  2:686284/236‑1 (MQ=255)
                                                                                                                                                 aaCAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCtt                                                                       <  1:733673/233‑1 (MQ=255)
                                                                                                                                                 aaCAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGa                                                                     <  1:194746/235‑1 (MQ=255)
                                                                                                                                                 aaCAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAg                                                                    <  1:385475/236‑1 (MQ=255)
                                                                                                                                                 aaCAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAg                                                                    <  2:581649/236‑1 (MQ=255)
                                                                                                                                                 aaCAGGATTTCCCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCtt                                                                       <  1:628260/233‑1 (MQ=255)
                                                                                                                                                   ctggATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGa                                                                  <  2:343686/234‑1 (MQ=255)
                                                                                                                                                    aGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGaa                                                                 <  1:368684/236‑1 (MQ=255)
                                                                                                                                                          tctcCCTGCGGTGTAAAGGGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGa                                                                  <  1:457290/229‑1 (MQ=255)
                                                                                                                                                                 gCGGTGAAAGGCGTAGCGGCTGGCTCTTACGCACAAATGGCCGGAAGCCAAGGCGTTTTTCCAGATCGCTACACTGGTGAGACGGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTCCTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGAATATACAGTAACCg                                                    <  2:509068/236‑1 (MQ=255)
                                                                                                                                                                             gTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGAATATACAGTAACCGCCAATTATGGAt                                        <  1:551095/236‑1 (MQ=255)
                                                                                                                                                                                                          cGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGAATATACAGTAACCGCCAATTATGGATGTGTAAACATCTGGACGGCTAAAATcc             >  2:470406/1‑234 (MQ=255)
                                                                                                                                                                                                             aaGCCAGGGCTTTGTTCCAGATCGCTACCCTGGTGGGCCGGGGCGGATTGCGTCTGATGCAACGTCGCCGCGGCGGCTGCGAGCGAGCCGCAGTTCCGAAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGAATATACAGTAACCGCCAATTATGGATGTGTAAACATCTGGACGGCTAAAATCCTTc          <  1:319763/234‑1 (MQ=255)
                                                                                                                                                                                                             aaGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGAATATACAGTAACCGCCAATTATGGATGTGTAAACATCTGGACGGCTAAAATCCt            >  1:505249/1‑232 (MQ=255)
                                                                                                                                                                                                             aaGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGAATATACAGTAACCGCCAATTATGGATGTGTAAACATCTGGACGGCTAAAATCCTTCg         <  1:470406/235‑1 (MQ=255)
                                                                                                                                                                                                                   aGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCCGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGAATATACAGTAACCGCCAATTATGGATGTGTAAACATCTGGACGGCTAAAATCCTTCGACTTTTa  >  1:763186/1‑236 (MQ=255)
                                                                                                                                                                                                                                     cTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGa                                                                  >  1:476091/1‑154 (MQ=255)
                                                                                                                                                                                                                                     cTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGa                                                                  <  2:476091/154‑1 (MQ=255)
                                                                                                                                                                                                                                     cTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGAATATACAGTAACCGCCAATTa                                             >  1:646669/1‑175 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                      
GTTCTTATGGAAATTTTCTAACGCGGGTGTCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTAAACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCCGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTTTTACTTCGATCATGAAAGTCCTTCACTTCGGCATGAATAATTTGCGCTTGAGGAATATACAGTAACCGCCAATTATGGATGTGTAAACATCTGGACGGCTAAAATCCTTCGTCTTTTA  >  NC_000913/4012475‑4012921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A