Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,012,707 A→C F37C (TTT→TGT)  metR ← methionine biosynthesis regulon transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,012,7070AC98.6% 780.1 / ‑2.2 219F37C (TTT→TGT) metRmethionine biosynthesis regulon transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (3/0);  new base C (114/101);  total (117/102)
Fisher's exact test for biased strand distribution p-value = 2.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 225 total aligned reads displayed.

TGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTAAACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTT  >  NC_000913/4012610‑4012803
                                                                                                 |                                                                                                 
tGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTacac                                                                                                 <  2:5214765/100‑1 (MQ=255)
tGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTacac                                                                                                 <  2:3633721/100‑1 (MQ=255)
tGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTacac                                                                                                 <  2:2463248/100‑1 (MQ=255)
tGCCAGTGGCAACAGGATTTCCCCCTGCGGTGTAAAGTGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCGAAGGAGTTGTTCCTGATCGCTacac                                                                                                 <  2:4126232/100‑1 (MQ=255)
 gCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACt                                                                                                >  2:3485808/1‑100 (MQ=255)
 gCCAGTTCCAACAGGATTTCTCCCTGCGGGGTAAAGCGTAGCGGCTGGCTCTTACGGACAAATAGCCGGAACGCAAGGCGTTGTTCCAGCTCGTTACACt                                                                                                >  2:4534118/1‑100 (MQ=255)
   cAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTgg                                                                                              >  2:947352/1‑100 (MQ=255)
     gTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTg                                                                                            <  1:946365/100‑1 (MQ=255)
     gTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTg                                                                                            >  1:1032204/1‑100 (MQ=255)
     gTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTg                                                                                            <  1:442275/100‑1 (MQ=255)
       tGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTgag                                                                                          <  2:4722011/100‑1 (MQ=255)
       tGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTgag                                                                                          <  2:4648995/100‑1 (MQ=255)
        gCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTgaga                                                                                         >  2:1256557/1‑100 (MQ=255)
         ctaCAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACTAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGAc                                                                                        <  2:5468088/98‑1 (MQ=255)
           aCAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAg                                                                                      >  1:5394251/1‑100 (MQ=255)
            cAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAgg                                                                                     >  1:1396404/1‑100 (MQ=255)
            cAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAgg                                                                                     >  1:3448216/1‑100 (MQ=255)
             aGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAggg                                                                                    >  1:3659601/1‑100 (MQ=255)
             aGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAggg                                                                                    <  1:2772011/100‑1 (MQ=255)
              ggATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGc                                                                                   >  1:4467475/1‑100 (MQ=255)
                aTTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCgg                                                                                 <  1:638970/100‑1 (MQ=255)
                aTTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCgg                                                                                 <  1:3967724/100‑1 (MQ=255)
                aTTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCgg                                                                                 <  2:22413/100‑1 (MQ=255)
                 tttCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGa                                                                                <  1:4547036/100‑1 (MQ=255)
                   tctcCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGAtt                                                                              >  1:3324986/1‑100 (MQ=255)
                       ccTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGt                                                                          <  1:2361992/100‑1 (MQ=255)
                        cTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTc                                                                         >  2:2788762/1‑100 (MQ=255)
                        cTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTc                                                                         <  2:4491709/100‑1 (MQ=255)
                         tGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCt                                                                        >  2:3551076/1‑100 (MQ=255)
                           cGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGa                                                                      <  2:1018511/100‑1 (MQ=255)
                           cGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGa                                                                      <  1:2620150/100‑1 (MQ=255)
                             gtgtAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATg                                                                    <  2:4239608/100‑1 (MQ=255)
                              tgtAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGc                                                                   <  1:2941565/100‑1 (MQ=255)
                               gtAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCa                                                                  >  1:2834778/1‑100 (MQ=255)
                               gtAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCa                                                                  >  2:1958698/1‑100 (MQ=255)
                                tAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCaa                                                                 <  2:2861575/100‑1 (MQ=255)
                                tAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCaa                                                                 >  1:1539200/1‑100 (MQ=255)
                                 aaaGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAAc                                                                <  1:2650535/100‑1 (MQ=255)
                                 aaaGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAAc                                                                <  2:3792780/100‑1 (MQ=255)
                                 aaaGCGTAGCGGCTGGCTATCACGCACAAATAGCCGGAAGGCAAGGCGTTGTTCCAGACCGCTACCCTGGTGAGACAGGACGGAATGCGTCTGACGCAAc                                                                <  2:600152/100‑1 (MQ=255)
                                  aaGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGCGACAGGGCGGATTGCGTCTGATGCAACg                                                               >  2:3702114/1‑100 (MQ=255)
                                  aaGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACg                                                               >  1:3492852/1‑100 (MQ=255)
                                      gTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTcgc                                                           >  1:205813/1‑100 (MQ=255)
                                        aGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTcgc‑cg                                                        >  2:4224284/1‑100 (MQ=255)
                                         gCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTcgc‑cgc                                                       <  1:3795451/100‑1 (MQ=255)
                                           ggctggctCTTACGCATAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAg                                                     >  2:667594/1‑100 (MQ=255)
                                           ggctggctCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAg                                                     >  2:3088782/1‑100 (MQ=255)
                                              tggctCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCgg                                                  >  1:5533736/1‑100 (MQ=255)
                                                gctCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCt                                                <  2:1701885/100‑1 (MQ=255)
                                                 ctctTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTg                                               >  1:4651887/1‑100 (MQ=255)
                                                       cGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTgcgagcg                                         >  1:509846/1‑100 (MQ=255)
                                                         cacaAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCTCGCAGCCGCTgcggcgac                                        <  1:907296/100‑6 (MQ=255)
                                                         cacaAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGcgagcgag                                       >  2:2476380/1‑100 (MQ=255)
                                                         cacaAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGcgagcgag                                       <  1:2351287/100‑1 (MQ=255)
                                                           caAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGcc                                     >  1:3951809/1‑100 (MQ=255)
                                                           caAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGcc                                     >  1:1258030/1‑100 (MQ=255)
                                                           caAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGcc                                     <  2:4833899/100‑1 (MQ=255)
                                                             aaTAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGc                                   <  2:4108915/100‑1 (MQ=255)
                                                             aaTAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGc                                   <  2:1377794/100‑1 (MQ=255)
                                                             aaTAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGc                                   >  2:1333210/1‑100 (MQ=255)
                                                                aGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGt                                <  1:4820119/100‑1 (MQ=255)
                                                                 gCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGtt                               >  1:3240853/1‑100 (MQ=255)
                                                                  cctgAAGCCAAGGCGTTGTTCCAGATCGCTAAACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTc                              >  2:3758807/4‑100 (MQ=255)
                                                                  ccGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTc                              <  1:3089672/100‑1 (MQ=255)
                                                                   cGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCTACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTcc                             >  1:5246295/1‑100 (MQ=255)
                                                                   cGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTcc                             <  1:3303907/100‑1 (MQ=255)
                                                                    ggAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCg                            >  1:411720/1‑100 (MQ=255)
                                                                     gAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGc                           <  1:4187296/100‑1 (MQ=255)
                                                                     gAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGc                           >  1:1603650/1‑100 (MQ=255)
                                                                     gAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGc                           <  1:1489844/100‑1 (MQ=255)
                                                                      aaGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCa                          <  1:1124835/100‑1 (MQ=255)
                                                                         ccAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACg                       >  2:3209145/1‑100 (MQ=255)
                                                                         ccAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACg                       >  1:5354087/1‑100 (MQ=255)
                                                                         ccAAGGCGTTGTTCCAGATCGCTAAACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACg                       >  1:2852367/1‑100 (MQ=255)
                                                                          cAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGc                      >  2:831880/1‑100 (MQ=255)
                                                                           aaGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGCGCGAGCCGCAGTTCCGCAACGCt                     >  2:4985109/1‑100 (MQ=255)
                                                                           aaGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCt                     >  1:4354593/1‑100 (MQ=255)
                                                                              gCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGt                  >  1:297462/1‑100 (MQ=255)
                                                                               cGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTa                 >  1:2065125/1‑100 (MQ=255)
                                                                                  tgttCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCg              >  2:5068690/1‑100 (MQ=255)
                                                                                  tgttCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCg              >  2:2077934/1‑100 (MQ=255)
                                                                                   gttCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGt             >  1:2796533/1‑100 (MQ=255)
                                                                                   gttCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGt             >  2:5393371/1‑100 (MQ=255)
                                                                                    ttCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGtt            >  1:1576020/1‑100 (MQ=255)
                                                                                    ttCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGtt            >  2:2747232/1‑100 (MQ=255)
                                                                                    ttCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGtt            >  2:1829149/1‑100 (MQ=255)
                                                                                     tCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGttt           <  1:2140394/100‑1 (MQ=255)
                                                                                     tCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGttt           <  2:1519958/100‑1 (MQ=255)
                                                                                      ccAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGtttt          >  2:3894928/1‑100 (MQ=255)
                                                                                       cAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTc         <  2:268846/100‑1 (MQ=255)
                                                                                        ggATCGCTACACTGGTGGGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCa        <  2:4424390/99‑1 (MQ=255)
                                                                                        aGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCa        >  1:2421093/1‑100 (MQ=255)
                                                                                         gATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAg       <  2:1996094/100‑1 (MQ=255)
                                                                                         gATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAg       >  1:1838734/1‑100 (MQ=255)
                                                                                         gATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAg       <  2:3935652/100‑1 (MQ=255)
                                                                                         gATCGCTACACTGGTGAGACAGGGCGGAGTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAg       >  1:4895896/1‑100 (MQ=255)
                                                                                          aTCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAgg      <  2:760055/100‑1 (MQ=255)
                                                                                          aTCGCTACACTGGTGAGACAGGGCGGATTGCGCCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCGTGTAGCGTGTTCAgg      >  2:2951910/1‑100 (MQ=255)
                                                                                            cGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGgtg    <  2:3436828/100‑1 (MQ=255)
                                                                                             gCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGgtgt   >  1:1938758/1‑100 (MQ=255)
                                                                                                 |                                                                                                 
TGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTAAACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTT  >  NC_000913/4012610‑4012803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A