Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,012,707 A→C F37C (TTT→TGT)  metR ← methionine biosynthesis regulon transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,012,7070AC99.5% 717.0 / ‑4.5 202F37C (TTT→TGT) metRmethionine biosynthesis regulon transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (117/84);  total (118/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

Only 100 of 204 total aligned reads displayed.

TGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTAAACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTT  >  NC_000913/4012610‑4012803
                                                                                                 |                                                                                                 
tGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTacac                                                                                                 <  2:2771311/100‑1 (MQ=255)
tGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTacac                                                                                                 <  1:1575423/100‑1 (MQ=255)
 gCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACt                                                                                                >  1:2082195/1‑100 (MQ=255)
 gCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGAAGCGGCTAGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGCTGTTCCAGATCGCTACACt                                                                                                >  1:3619685/1‑100 (MQ=255)
  ccAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTATTACGCACAAATAGACGGAAGCCAAGGAGTTGTTCCAGATCGCTACACTg                                                                                               >  2:1430016/1‑100 (MQ=255)
   cAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTgg                                                                                              >  1:3000609/1‑100 (MQ=255)
   cAGTTGCAACAGGATTTCTCCCTGCGGGGTAACGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTgg                                                                                              >  1:1907356/1‑100 (MQ=255)
   aaGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTgg                                                                                              >  2:1608446/2‑100 (MQ=255)
     gTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTg                                                                                            <  1:1358927/100‑1 (MQ=255)
     gTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTg                                                                                            <  1:2705364/100‑1 (MQ=255)
     gTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTg                                                                                            >  1:497244/1‑100 (MQ=255)
         cAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGAc                                                                                        <  2:2427143/100‑1 (MQ=255)
         cAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGAc                                                                                        >  1:3638193/1‑100 (MQ=255)
         cAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTAAACTGGTGAGAc                                                                                        >  2:3568515/1‑100 (MQ=255)
          aaCAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACa                                                                                       <  1:1435058/100‑1 (MQ=255)
          aaCAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACa                                                                                       >  2:2946734/1‑100 (MQ=255)
           aCAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCAAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAg                                                                                      <  2:778097/100‑1 (MQ=255)
              tcttttCTCTCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGc                                                                                   <  2:3459449/97‑1 (MQ=255)
              ggATTTCTCCCTGCGGTGTAACGCGTAGCGGCTGGCTCTTACGCACAACTGGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTCAGCCCGGGc                                                                                   <  2:711952/100‑1 (MQ=255)
              ggATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGc                                                                                   <  1:3417955/100‑1 (MQ=255)
              ggATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGc                                                                                   >  1:1878115/1‑100 (MQ=255)
               gATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCg                                                                                  <  1:3229558/100‑1 (MQ=255)
                aTTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAggggtt                                                                                 >  1:2946105/1‑97 (MQ=255)
                aTTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCgg                                                                                 <  1:960510/100‑1 (MQ=255)
                 tttCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGa                                                                                >  1:2805848/1‑100 (MQ=255)
                 tttCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGa                                                                                >  2:70102/1‑100 (MQ=255)
                   tctcCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGAtt                                                                              >  2:2038097/1‑100 (MQ=255)
                   tctcCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGAtt                                                                              <  1:1111716/100‑1 (MQ=255)
                    ctcCCTGCGGTGTAAAGCGTAGCGGGTGGCTCTTACGCACAAATAGCGGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTg                                                                             >  1:3647409/1‑100 (MQ=255)
                    ctcCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTg                                                                             <  1:2193265/100‑1 (MQ=255)
                    ctcCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTg                                                                             >  1:1498322/1‑100 (MQ=255)
                    ctcCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTg                                                                             <  2:3121168/100‑1 (MQ=255)
                       ccTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGt                                                                          <  2:1827315/100‑1 (MQ=255)
                        cTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTc                                                                         <  1:1684713/100‑1 (MQ=255)
                        cTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTc                                                                         <  2:3094378/100‑1 (MQ=255)
                         tGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCt                                                                        <  1:1708605/100‑1 (MQ=255)
                          gCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTg                                                                       <  2:3888210/100‑1 (MQ=255)
                          gCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTg                                                                       <  2:2563472/100‑1 (MQ=255)
                           cGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGa                                                                      <  1:3851881/100‑1 (MQ=255)
                            ggTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGAt                                                                     >  2:20878/1‑100 (MQ=255)
                             gtgtAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATg                                                                    <  2:327702/100‑1 (MQ=255)
                             gtgtAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATg                                                                    <  2:2763409/100‑1 (MQ=255)
                              tgtAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGc                                                                   <  1:83921/100‑1 (MQ=255)
                              tgtAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGc                                                                   <  2:3680749/100‑1 (MQ=255)
                              tgtAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGc                                                                   >  1:2491623/1‑100 (MQ=255)
                                 aaaGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAAc                                                                >  1:2607318/1‑100 (MQ=255)
                                 aaaGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAAc                                                                >  1:3202662/1‑100 (MQ=255)
                                 aaaGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAAc                                                                >  2:2202666/1‑100 (MQ=255)
                                  aaGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACg                                                               <  2:543670/100‑1 (MQ=255)
                                  aaGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACg                                                               <  1:3847186/100‑1 (MQ=255)
                                   aGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGt                                                              >  2:506810/1‑100 (MQ=255)
                                    gCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTc                                                             >  1:1039262/1‑100 (MQ=255)
                                     cGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTcg                                                            >  1:214136/1‑100 (MQ=255)
                                     cGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTcg                                                            >  2:1497423/1‑100 (MQ=255)
                                      gTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTcgc                                                           >  1:940240/1‑100 (MQ=255)
                                       tAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTcgc‑c                                                         >  1:1761635/1‑100 (MQ=255)
                                       tAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTcgc‑c                                                         <  1:109119/100‑1 (MQ=255)
                                          cGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCACCGTAGC‑CGCa                                                      >  1:3450955/1‑100 (MQ=255)
                                           ggctggctCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAg                                                     >  1:2317894/1‑100 (MQ=255)
                                            gctggctCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGc                                                    >  2:2073260/1‑100 (MQ=255)
                                             ctggctCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCg                                                   >  1:382549/1‑100 (MQ=255)
                                                gctCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCt                                                >  1:1541714/1‑100 (MQ=255)
                                                       cGCACAAATCGCCGGAAGCCAACGCGTTGTTCCAGATCGCTACACTGGTGCGTCAGGGCCGATTGCGTCTGCTGCCACGTCGC‑CGCAGCGGCTgcgagcg                                         >  2:27051/1‑100 (MQ=255)
                                                       cGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGCTCGCAGCCGCTgcggcg                                          <  2:2998703/100‑4 (MQ=255)
                                                       cGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTgcgagcg                                         <  2:1017652/100‑1 (MQ=255)
                                                        gCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTgcgagcga                                        >  1:3151056/1‑100 (MQ=255)
                                                        gCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTgcgagcga                                        <  2:2678186/100‑1 (MQ=255)
                                                         cacaAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGcgagggag                                       >  2:2605091/1‑100 (MQ=255)
                                                         cacaAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGcgagcgag                                       >  1:1721935/1‑100 (MQ=255)
                                                           caAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGcc                                     >  1:614161/1‑100 (MQ=255)
                                                             aaTAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGc                                   <  1:2975240/100‑1 (MQ=255)
                                                              aTAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCa                                  >  1:2442502/1‑100 (MQ=255)
                                                              aTAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCa                                  >  1:3694401/1‑100 (MQ=255)
                                                                aGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGt                                >  2:3237781/1‑100 (MQ=255)
                                                                 gCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGtt                               >  1:2356614/1‑100 (MQ=255)
                                                                 gCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGtt                               >  1:1806007/1‑100 (MQ=255)
                                                                 gCCGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCAAGCGAGCCGCAGtt                               >  1:1610477/1‑100 (MQ=255)
                                                                  ccGGAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTc                              >  1:759751/1‑100 (MQ=255)
                                                                    ggAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCg                            <  1:920537/100‑1 (MQ=255)
                                                                    ggAAGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCg                            <  2:230780/100‑1 (MQ=255)
                                                                      aaGCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCa                          <  2:3312925/100‑1 (MQ=255)
                                                                        gCCAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAAc                        <  1:798141/100‑1 (MQ=255)
                                                                         ccAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACg                       >  1:3514430/1‑100 (MQ=255)
                                                                         ccAAGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACg                       >  2:188985/1‑100 (MQ=255)
                                                                          cAAGGCGTGGTGCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGAAGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGc                      <  2:1375684/100‑1 (MQ=255)
                                                                            aGGCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCtt                    <  2:461055/100‑1 (MQ=255)
                                                                             ggCGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTg                   >  1:222877/1‑100 (MQ=255)
                                                                             ggCGTTGTTCCAGATCACTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTg                   <  1:3355434/100‑1 (MQ=255)
                                                                               cGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTa                 >  1:2892974/1‑100 (MQ=255)
                                                                               cGTTGTTCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTa                 <  2:3359308/100‑1 (MQ=255)
                                                                                  tgttCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCg              <  1:3379634/100‑1 (MQ=255)
                                                                                   gttCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGt             >  1:1104970/1‑100 (MQ=255)
                                                                                   gttCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGt             >  1:324749/1‑100 (MQ=255)
                                                                                   gttCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGt             <  2:1732951/100‑1 (MQ=255)
                                                                                    ttCCAGATCGCTACACTGGTAAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCCAGCCGCAGTTCCGCAACGCTTGTAGCGtt            >  2:1073948/1‑100 (MQ=255)
                                                                                     tCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGttt           >  2:3744613/1‑100 (MQ=255)
                                                                                     tCCAGATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGttt           >  1:1992650/1‑100 (MQ=255)
                                                                                        aGATCTCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑GGCAGTGGTTGCGAGCGAGCCGCAGTTCCGCAACGCCTGTAGCGTTTTCa        <  1:3870213/100‑1 (MQ=255)
                                                                                         gATCGCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAg       <  1:1702045/100‑1 (MQ=255)
                                                                                             gCTACACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGgtgt   >  1:133947/1‑100 (MQ=255)
                                                                                                 |                                                                                                 
TGCCAGTTGCAACAGGATTTCTCCCTGCGGTGTAAAGCGTAGCGGCTGGCTCTTACGCACAAATAGCCGGAAGCCAAGGCGTTGTTCCAGATCGCTAAACTGGTGAGACAGGGCGGATTGCGTCTGATGCAACGTCGC‑CGCAGCGGCTGCGAGCGAGCCGCAGTTCCGCAACGCTTGTAGCGTTTTCAGGTGTT  >  NC_000913/4012610‑4012803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A