Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I52 R1	239	220.4	11079197	93.2%	10325811	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	162,349	A→C	Y82S (TAC→TCC)	hrpB →	putative ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	162,349	0	A	C	99.1%	774.4 / ‑2.2	217	Y82S (TAC→TCC)	hrpB	putative ATP‑dependent helicase
Reads supporting (aligned to +/- strand): ref base A (2/0); new base C (104/111); total (106/111)
Fisher's exact test for biased strand distribution p-value = 2.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

Only 100 of 219 total aligned reads displayed.

AAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTACCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACT  >  NC_000913/162252‑162445

aaaaTTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTccc                                                                                                <  2:3352770/100‑1 (MQ=255)

aaaaTTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTccc                                                                                                <  1:5489622/100‑1 (MQ=255)

   aTTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGa                                                                                             <  1:2892569/100‑1 (MQ=255)

    ttATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTTGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAt                                                                                            <  2:5015074/100‑1 (MQ=255)

    ttATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAt                                                                                            <  2:2610887/100‑1 (MQ=255)

    ttATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAt                                                                                            <  1:3783368/100‑1 (MQ=255)

       tCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAtgcg                                                                                         >  1:3276048/1‑100 (MQ=255)

        ccTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAtgcgt                                                                                        >  1:578879/1‑100 (MQ=255)

         ctgctgGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAtgcgtg                                                                                       >  2:4320667/1‑100 (MQ=255)

         ctgctgGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAtgcgtg                                                                                       <  1:909973/100‑1 (MQ=255)

         ctgctgGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAtgcgtg                                                                                       >  1:983815/1‑100 (MQ=255)

         ctgctgGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAtgcgtg                                                                                       <  2:136674/100‑1 (MQ=255)

          tgctgGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAtgcgtgc                                                                                      >  1:2329030/1‑100 (MQ=255)

          tgctgGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAtgcgtgc                                                                                      >  1:5267967/1‑100 (MQ=255)

               gAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCaaa                                                                                 >  1:1968995/1‑100 (MQ=255)

                aGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCaaaa                                                                                >  2:5275097/1‑100 (MQ=255)

                 gCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAAc                                                                               <  1:1097252/100‑1 (MQ=255)

                 gCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAAc                                                                               <  1:2798848/100‑1 (MQ=255)

                   cgcgTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTg                                                                             >  2:1243737/1‑100 (MQ=255)

                   cgcgTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGACTCCCGGATGCGTGCGCAAAACTg                                                                             >  1:556517/1‑100 (MQ=255)

                      gtcgtcTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGt                                                                          <  2:1304746/100‑1 (MQ=255)

                      gtcgtcTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGt                                                                          >  1:1279311/1‑100 (MQ=255)

                       tcgtcTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTc                                                                         <  2:2519459/100‑1 (MQ=255)

                        cgtcTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCg                                                                        <  1:716137/100‑1 (MQ=255)

                         gtcTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCgg                                                                       <  1:5347400/100‑1 (MQ=255)

                         gtcTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCgg                                                                       >  2:5409755/1‑100 (MQ=255)

                         gtcTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCgg                                                                       >  2:1880744/1‑100 (MQ=255)

                          tcTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCggg                                                                      >  1:1663015/1‑100 (MQ=255)

                           cTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGc                                                                     <  2:2570679/100‑1 (MQ=255)

                           cTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGc                                                                     <  1:4939821/100‑1 (MQ=255)

                           cTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGc                                                                     <  2:1845834/100‑1 (MQ=255)

                            tGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGcc                                                                    <  2:3754029/100‑1 (MQ=255)

                               cggcgCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGaa                                                                 <  2:3237316/100‑1 (MQ=255)

                                ggcgCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAAt                                                                >  2:481117/1‑100 (MQ=255)

                                ggcgCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAAt                                                                <  1:4361254/100‑1 (MQ=255)

                                  cgcgTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATAc                                                              >  2:2006360/1‑100 (MQ=255)

                                  cgcgTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATAc                                                              <  1:3711375/100‑1 (MQ=255)

                                    cgTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATAccc                                                            <  2:397864/100‑1 (MQ=255)

                                    cgTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATAccc                                                            >  1:1795901/1‑100 (MQ=255)

                                       aaCGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGcc                                                         <  2:231595/100‑1 (MQ=255)

                                         cGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTg                                                       <  2:2669346/100‑1 (MQ=255)

                                         cGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTg                                                       <  1:3978925/100‑1 (MQ=255)

                                          gTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTgg                                                      >  1:2095197/1‑100 (MQ=255)

                                          gTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTgg                                                      >  1:2590383/1‑100 (MQ=255)

                                          gTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTgg                                                      >  1:1318711/1‑100 (MQ=255)

                                          gTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGAATGCGCGCGCAAAACTGCGTCGGGCCGAATACCCGCCTgg                                                      >  2:5251062/1‑100 (MQ=255)

                                           tCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGa                                                     <  1:620198/100‑1 (MQ=255)

                                            cgcgCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGaa                                                    >  2:4428359/1‑100 (MQ=255)

                                            cgcgCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGaa                                                    <  2:1928155/100‑1 (MQ=255)

                                             gcgcAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAg                                                   <  1:408568/100‑1 (MQ=255)

                                              cgcAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGAAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGt                                                  <  2:3096086/100‑1 (MQ=255)

                                               gcAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTg                                                 >  2:1779458/1‑100 (MQ=255)

                                                cAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTgg                                                <  1:3350861/100‑1 (MQ=255)

                                                 aaCGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGt                                               <  2:484398/100‑1 (MQ=255)

                                                 aaCGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGt                                               <  2:5064278/100‑1 (MQ=255)

                                                 aaCGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGt                                               <  2:4207099/100‑1 (MQ=255)

                                                     gCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGGGTGCGCAAAACTCCGTCGGGCCGAATACCCGCCTGGAAGTGGTTAcc                                           <  2:239662/100‑1 (MQ=255)

                                                     cttGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTAcc                                           <  1:838914/98‑1 (MQ=255)

                                                        ggCGAAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGaa                                        >  2:4658346/1‑100 (MQ=255)

                                                         gagaaGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAg                                       <  1:1265913/96‑1 (MQ=255)

                                                         gCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAg                                       >  1:3894828/1‑100 (MQ=255)

                                                          cGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAgg                                      <  1:1987736/100‑1 (MQ=255)

                                                           ggAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGc                                     <  1:3411458/100‑1 (MQ=255)

                                                              gctgctTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTg                                  >  1:3619992/1‑100 (MQ=255)

                                                               ctgctTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGc                                 <  2:1087514/100‑1 (MQ=255)

                                                                 gctTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTg                               >  1:821692/1‑100 (MQ=255)

                                                                 gctTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTg                               >  1:2694894/1‑100 (MQ=255)

                                                                 gctTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTg                               >  2:4450770/1‑100 (MQ=255)

                                                                  ctTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGa                              <  2:731122/100‑1 (MQ=255)

                                                                     aaCGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGAcgc                           <  1:520823/100‑1 (MQ=255)

                                                                      aCGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGAcgcg                          >  1:1373560/1‑100 (MQ=255)

                                                                      aCGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGAcgcg                          <  1:2274003/100‑1 (MQ=255)

                                                                      aCGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGAcgcg                          <  1:2959740/100‑1 (MQ=255)

                                                                      aCGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGAcgcg                          >  2:3832176/1‑100 (MQ=255)

                                                                       cGAAACGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTCCGTCGGGCCCAATGCCCCCCTGGCAGTCGTTACCGAAGGCGTGCCGACgcgc                         >  2:2936922/1‑100 (MQ=255)

                                                                       cGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGCCgcgc                         >  1:3095822/1‑100 (MQ=255)

                                                                       cGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACgcgc                         >  1:683985/1‑100 (MQ=255)

                                                                       cGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCAGCCTGGAAGTGGTTACCGAAGGCGTGCTGACgcgc                         >  1:2520611/1‑100 (MQ=255)

                                                                        gAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCa                        <  1:5098567/100‑1 (MQ=255)

                                                                         aaaaGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCAt                       <  1:2549046/100‑1 (MQ=255)

                                                                           aaGCCAGGCTATACCGTTGGCTCGCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCTGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGa                     <  1:4860345/100‑1 (MQ=255)

                                                                            aGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGAt                    <  2:3435341/100‑1 (MQ=255)

                                                                            aGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGAt                    >  2:3961091/1‑100 (MQ=255)

                                                                            aGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGACCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGAt                    >  2:2446266/1‑100 (MQ=255)

                                                                              ccAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATcc                  >  2:1594487/1‑100 (MQ=255)

                                                                              ccAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATcc                  <  1:4464484/100‑1 (MQ=255)

                                                                               cAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCa                 <  1:78041/100‑1 (MQ=255)

                                                                               cAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCa                 >  1:1421714/1‑100 (MQ=255)

                                                                                aGGCGATACCGTGGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGAAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAg                >  2:5289099/1‑100 (MQ=255)

                                                                                 ggCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGc               >  2:2229272/1‑100 (MQ=255)

                                                                                  gCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCg              <  2:2073022/100‑1 (MQ=255)

                                                                                    gATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTg            <  2:2807882/100‑1 (MQ=255)

                                                                                     aTACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGa           <  1:3185047/100‑1 (MQ=255)

                                                                                        ccGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGAccc        >  2:3246860/1‑100 (MQ=255)

                                                                                         cGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCg       <  1:286066/100‑1 (MQ=255)

                                                                                         cGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCg       >  2:3210218/1‑100 (MQ=255)

                                                                                            tGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGaa    <  1:4180499/100‑1 (MQ=255)

                                                                                             ggCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAAc   <  2:3700408/100‑1 (MQ=255)

                                                                                             ggCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAAc   <  1:244155/100‑1 (MQ=255)

                                                                                              gCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACt  >  2:4887318/1‑100 (MQ=255)

AAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTACCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACT  >  NC_000913/162252‑162445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A