Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 162,349 A→C Y82S (TAC→TCC)  hrpB → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913162,3490AC99.4% 572.7 / NA 161Y82S (TAC→TCC) hrpBputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (81/79);  total (81/80)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 161 total aligned reads displayed.

AAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTACCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAAC  >  NC_000913/162253‑162444
                                                                                                |                                                                                               
aaaTTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCg                                                                                              <  1:1368794/100‑1 (MQ=255)
  aTTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGa                                                                                            <  2:3433626/100‑1 (MQ=255)
   ttATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAt                                                                                           >  2:394368/1‑100 (MQ=255)
    tATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAtg                                                                                          <  2:2995804/100‑1 (MQ=255)
    tATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAtg                                                                                          <  1:401807/100‑1 (MQ=255)
     aTCCTGCTGGAGCCTCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAtgc                                                                                         <  1:3310383/100‑1 (MQ=255)
       ccTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAtgcgt                                                                                       >  1:943892/1‑100 (MQ=255)
        ctgctgGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAtgcgtg                                                                                      <  1:3274261/100‑1 (MQ=255)
        ctgctgGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAtgcgtg                                                                                      >  1:1385766/1‑100 (MQ=255)
        ctgctgGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAtgcgtg                                                                                      <  1:43680/100‑1 (MQ=255)
         tgctgGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAtgcgtgc                                                                                     <  1:1105692/100‑1 (MQ=255)
           ctgGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTgcgc                                                                                   <  1:2293244/100‑1 (MQ=255)
           ctgGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTgcgc                                                                                   >  2:1792324/1‑100 (MQ=255)
             ggAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCaa                                                                                 >  1:3077710/1‑100 (MQ=255)
               aGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCaaaa                                                                               >  2:3235194/1‑100 (MQ=255)
                gCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAAc                                                                              >  2:1078488/1‑100 (MQ=255)
                gCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAAc                                                                              <  2:2462052/100‑1 (MQ=255)
                  cgcgTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTg                                                                            >  2:1519815/1‑100 (MQ=255)
                   gcgTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGc                                                                           >  1:4066489/1‑100 (MQ=255)
                   gcgTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGc                                                                           >  1:2181309/1‑100 (MQ=255)
                     gtcgtcTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCCTTGGCCCCCGGATGCGTGCGCAAAACTGCGt                                                                         >  2:3247735/1‑100 (MQ=255)
                      tcgtcTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTc                                                                        <  2:1332945/100‑1 (MQ=255)
                       cgtcTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCg                                                                       >  2:1478594/1‑100 (MQ=255)
                        gtcTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCgg                                                                      <  2:1678941/100‑1 (MQ=255)
                        gtcTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCgg                                                                      >  1:1061995/1‑100 (MQ=255)
                         tcTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCggg                                                                     >  1:3766788/1‑100 (MQ=255)
                          cTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCgggn                                                                    <  1:3250142/100‑2 (MQ=255)
                          cTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGc                                                                    <  2:604287/100‑1 (MQ=255)
                          cTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGc                                                                    >  1:2811974/1‑100 (MQ=255)
                           tGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAGAACTGCGCCAGGcc                                                                   >  2:3651780/1‑100 (MQ=255)
                            ggcggcGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCg                                                                  <  2:262304/100‑1 (MQ=255)
                            ggcggcGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCg                                                                  <  2:1610299/100‑1 (MQ=255)
                            ggcggcGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCg                                                                  >  2:1063717/1‑100 (MQ=255)
                            ggcggcGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCg                                                                  >  2:1266728/1‑100 (MQ=255)
                             gcggcgCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGa                                                                 <  1:3695708/100‑1 (MQ=255)
                             gcggcgCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGa                                                                 <  1:668705/100‑1 (MQ=255)
                              cggcgCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGaa                                                                >  1:2512643/1‑100 (MQ=255)
                              cggcgCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGaa                                                                <  1:2184329/100‑1 (MQ=255)
                                    gTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCg                                                          >  2:3188007/1‑100 (MQ=255)
                                      aaCGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGcc                                                        <  1:2573865/100‑1 (MQ=255)
                                        cGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTg                                                      <  1:1717841/100‑1 (MQ=255)
                                         gTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTgg                                                     >  2:2257644/1‑100 (MQ=255)
                                         gTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTgg                                                     <  1:2054749/100‑1 (MQ=255)
                                          tCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGa                                                    <  2:1487800/100‑1 (MQ=255)
                                            gcgcAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAg                                                  <  1:2584881/100‑1 (MQ=255)
                                             cgcAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGt                                                 >  1:1554563/1‑100 (MQ=255)
                                               cAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTgg                                               <  2:1288856/100‑1 (MQ=255)
                                                  cGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTa                                            >  1:2948867/1‑100 (MQ=255)
                                                  cGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCACAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTa                                            >  1:3459878/1‑100 (MQ=255)
                                                   ggCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTAc                                           <  1:655500/100‑1 (MQ=255)
                                                   ggCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTAc                                           <  2:1672430/100‑1 (MQ=255)
                                                         cGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACCGCGCCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAgg                                     <  2:3754924/100‑1 (MQ=255)
                                                           gAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCg                                   <  1:1360962/100‑1 (MQ=255)
                                                             gctgctTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTg                                 <  1:1100593/100‑1 (MQ=255)
                                                             gctgctTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTg                                 >  1:2446003/1‑100 (MQ=255)
                                                              ctgctTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGc                                >  1:181431/1‑100 (MQ=255)
                                                                gctTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTg                              >  2:2344615/1‑100 (MQ=255)
                                                                gctTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTACGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTg                              >  2:2862495/1‑100 (MQ=255)
                                                                  ttAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGCCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGAc                            >  2:585273/1‑100 (MQ=255)
                                                                   tAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGAcg                           >  2:2921874/1‑100 (MQ=255)
                                                                   tAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGAcg                           <  2:1040181/100‑1 (MQ=255)
                                                                   tAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGAcg                           >  1:3392837/1‑100 (MQ=255)
                                                                   tAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCAGatc                           >  2:4284/1‑98 (MQ=255)
                                                                      cGAAAGGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGCCCGAATACCCGCCCGGAAGTGGTTACCGAAGGCGTGCTGACgcgc                        >  2:3512755/1‑100 (MQ=255)
                                                                      cGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACgcgc                        >  2:2733554/1‑100 (MQ=255)
                                                                       gAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCa                       <  2:3453130/100‑1 (MQ=255)
                                                                       gAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCa                       <  2:3789124/100‑1 (MQ=255)
                                                                       gAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCa                       >  2:1155324/1‑100 (MQ=255)
                                                                        aaaaGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCAt                      <  1:1820795/100‑1 (MQ=255)
                                                                         aaaGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATg                     <  1:2732139/100‑1 (MQ=255)
                                                                          aaGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGa                    <  1:2420874/100‑1 (MQ=255)
                                                                          aaGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGa                    <  1:1156218/100‑1 (MQ=255)
                                                                           aGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGAAAGTGGTTACCGAAGGCGTGCTGACGCGCATGAt                   <  1:3007827/100‑1 (MQ=255)
                                                                             ccAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATcc                 >  2:1995272/1‑100 (MQ=255)
                                                                              cAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGACGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCa                >  1:3469084/1‑100 (MQ=255)
                                                                               cgccGATCCCGTTGCCGCCCGTATGGGTCCGCAAAACTGCCTTGGGCCGAATGCCCCACGGGAAGGTGTTACCGAAGGCCTGCTGACGCGCATGATCCaa               <  2:2569637/97‑2 (MQ=255)
                                                                                 gCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCg             <  2:717464/100‑1 (MQ=255)
                                                                                 gCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCg             <  1:1060548/100‑1 (MQ=255)
                                                                                 gCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCg             <  2:2760473/100‑1 (MQ=255)
                                                                                  cGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGt            <  1:1763481/100‑1 (MQ=255)
                                                                                  cGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGt            >  2:3269141/1‑100 (MQ=255)
                                                                                  cGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGt            >  1:2780383/1‑100 (MQ=255)
                                                                                  cGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGt            <  2:1925092/100‑1 (MQ=255)
                                                                                   gATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTg           >  1:3271559/1‑100 (MQ=255)
                                                                                    aTACCTTGGCCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCCCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCCGCCTGa          <  2:1569998/100‑1 (MQ=255)
                                                                                    aTACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGa          >  1:1560325/1‑100 (MQ=255)
                                                                                     tACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGAc         >  1:150515/1‑100 (MQ=255)
                                                                                      aCCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGAcc        >  1:3848710/1‑100 (MQ=255)
                                                                                       ccGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGAccc       >  1:1251925/1‑100 (MQ=255)
                                                                                       ccGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGAccc       >  1:3295591/1‑100 (MQ=255)
                                                                                       ccGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGAccc       >  2:3916343/1‑100 (MQ=255)
                                                                                       ccGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGAccc       >  1:3508842/1‑100 (MQ=255)
                                                                                        cGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCg      >  1:1870969/1‑100 (MQ=255)
                                                                                         nttGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCgg     >  2:3054339/2‑100 (MQ=255)
                                                                                         gTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCgg     >  1:3520275/1‑100 (MQ=255)
                                                                                         gTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCgg     >  1:1933556/1‑100 (MQ=255)
                                                                                          ttGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGa    <  2:3598492/100‑1 (MQ=255)
                                                                                           tGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGaa   <  2:3143663/100‑1 (MQ=255)
                                                                                            ggCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAAc  >  1:2844592/1‑100 (MQ=255)
                                                                                            ggCTCCCGGATGCGTGCGCAAAACTGCCTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAAc  <  1:1646825/100‑1 (MQ=255)
                                                                                                |                                                                                               
AAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTACCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAAC  >  NC_000913/162253‑162444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A