Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F236 I1 R1 155 93.2 2393300 97.8% 2340647 180.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 162,349 A→C Y82S (TAC→TCC)  hrpB → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913162,3490AC97.8% 121.8 / ‑6.4 46Y82S (TAC→TCC) hrpBputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (19/26);  total (19/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTGCTGCCGTCTTACCTGAATTACTTACCGCTCTCGATTGTGCGCCGCAGGTATTATTAAGTGCGCCGACCGGGGCCGGGAAAT‑CAACCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTACCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCATGAGCGCAGCTTGCAGGCGGATTTGGCGTTGGCGCTGTTACTCGATGTGCAACAAGGTCTGCGTGATGACCTTAAACTGCTGATTATGTCGGCTAC  >  NC_000913/162119‑162577
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                    
cGTTGCTGCCGTCTTACCTGAATTACTTACCGCTCTCGATTGTGCGCCGCAGGTATTATTAAGTGCGCCGACCGGGGCCGGGAAAT‑CAACCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCCGGCGATACCGTTGGCGCCCGGGtgc                                                                                                                                                                                                                              >  2:513852/1‑239 (MQ=255)
cGTTGCGGCCTTCTTACCGGAATTCCTTCCCGCTCTCGATTGTGCGCCGAAGGTATTATTAAGTGCGCCGACCGGGGCCGGGAAAT‑CAACCTGGCTGCGGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGTGCCGCGACGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAtgc                                                                                                                                                                                                                              <  2:134942/239‑1 (MQ=255)
   tgctgcCGTCTTACCTGAATTACTTACCGCTCTCGATTGTGCGCCGCAGGTATTATTAAGTGCGCCGACCGGGGCCGGGAAAT‑CAACCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGACGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATCACGGTGGCTCTCGGAtgggtg                                                                                                                                                                                                                           >  2:1127502/1‑239 (MQ=255)
      tgcCGTCTTACCTGAATTACTTACCGCTCTCGATTGTGCGCCGCAGGTATTATTAAGTGCGCCGACCGGGGCCGGGAAAT‑CAACCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAACGCCAGGCGATACCGTTGGCTCCCGGATGCGTgcgc                                                                                                                                                                                                                        >  1:683950/1‑239 (MQ=255)
      tgcCGTCTTACCAGAATTACGTCGCGCTCTCGATTGTGCGCCGCAGGTATTATTAAGTGCGCCGACCGGGGCCGGGAAAT‑CAACCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTAGCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGAtgcgtgcg                                                                                                                                                                                                                         <  2:356801/238‑1 (MQ=255)
        ccGTCTTACCTGAATTACTTACCGCTCTCGATTGTGCGCCGCAGGTATTATTAAGTGCGCCGACCGGGGCCGGGAAAT‑CAACCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCaa                                                                                                                                                                                                                      >  1:946739/1‑239 (MQ=255)
                  tGAATTACTTACCGCTCTCGATTGTGCGCCGCAGGTATTATTAAGTGCGCCGACCGGGGCCGGGAAAT‑CAACCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCg                                                                                                                                                                                                            <  2:946739/239‑1 (MQ=255)
                  tGAATTACTTACCGCTCTCGATTGTGCGCCGCAGGTATTATTAAGTGCGCCGACCGGGGCCGGGAAAT‑CAACCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGACACCGTTGGCGCCCGGATGGGGGGGCAAAACAGccgcg                                                                                                                                                                                                            >  2:280856/1‑235 (MQ=255)
                                 tctcGATTGTGCGCCGCAGGTATTATTAAGTGCGCCGACCGGGGCCGGGAAAT‑CAACCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGGTACCGGTGGCTCCCGGGTGCGTGCGCAAAACTGCGCCGGGCCGAATACACg                                                                                                                                                                                               >  2:980049/1‑237 (MQ=255)
                                   tcGATTGTGCGCCGCAGGTATTATTAAGTGCGCCGACCGGGGCCGGGAAAT‑CAACCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCg                                                                                                                                                                                               >  1:774544/1‑235 (MQ=255)
                                                 cAGGTATTATTAAGTGCGCCGACCGGGGCCGGGAAAT‑CAACCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGa                                                                                                                                                                             >  1:394061/1‑239 (MQ=255)
                                                          ttaAGTGCGCCGCCCGGGGCCGGAAAAT‑CACCCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTACCGGGAAAATTATCCGGCGGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGGTGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCt                                                                                                                                                                    <  2:729545/239‑1 (MQ=255)
                                                            aggggcgcCGACCGGGGCGGAGAATG‑CAACCAGGCGCCCCATGAAAGTGCTGGCCGCTCCCGGCATTAACGGGAAAATTTTCCGGCTGGGGCCGCGTCGTCGGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGAAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGa                                                                                                                                                                  <  2:1045746/235‑1 (MQ=255)
                                                            aaGTGCGCCGACCGGGGCCGGAAAAT‑CAACCGGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTACCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGa                                                                                                                                                                  <  2:1073179/239‑1 (MQ=255)
                                                                 cgcCGACCGGGGCCGGGAAAT‑CAACCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGCGGTTACCGCAGGCGCGCTGAcccg                                                                                                                                                              >  1:112971/1‑238 (MQ=255)
                                                                   ccGACCGGGGCCGGGAAAT‑CAACCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGcc                                                                                                                                                                                                        <  2:261054/194‑1 (MQ=255)
                                                                   ccGACCGGGGCCGGGAAAT‑CAACCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGcc                                                                                                                                                                                                        >  1:261054/1‑194 (MQ=255)
                                                                              cGGGAAATCCAACCGGGCGGCCGCTGCACGTGCTGGCGCATCCCGGCATTACCGGGAAATTTATCCTGCGGGGGCCGCGTCGTCGGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGGGCTGCTTAACGAAAAGCCCGGCGATACCGTTGGCTCCCGGGTGAGAGGGCAAAACGGGGCAGGGCCGAATGCCCGCCTGGAAGTGGTTCCCGAAGGCGTGCTGACGCGCATGACCCAGca                                                                                                                                                  <  2:184752/238‑2 (MQ=255)
                                                                                  aaaT‑CAACCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGAcc                                                                                                                                             <  2:394061/238‑1 (MQ=255)
                                                                                            tGGCTGCAGCTGCAACTGCTGGCTCGCCCGGGCATAAACGGGAAATTTATCCGCCGGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGGGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGGGGGCAAAACGGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAg                                                                                                                                   <  2:361004/239‑1 (MQ=255)
                                                                                            ggtcTGCCGCTGCAACTGCTGCCGCAGCCCGGCATATACGGGAAAATTATCCTGCTGGGGCCGCGTCGTCGGGCGGCGCGTACCGTCGCGCAGCGGCTGGGGGAGCTGCTTAACGAAAAGCCGGGCGATACCGTTGGCTCCCGGATGCGTGCGGAAAACTGCGTCGGGCCGAATCCCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAg                                                                                                                                   <  2:1109785/236‑1 (MQ=255)
                                                                                            gggCTGCCGCGGCACCTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGGGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCACCGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCGGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAg                                                                                                                                   <  1:819283/238‑1 (MQ=255)
                                                                                                    gCTGCAACTGCTGGCGCCTCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATCCCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTg                                                                                                                           <  1:280856/239‑1 (MQ=255)
                                                                                                                ggCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGGGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGTGGTGGTTACCGAAGTCGTCCTGGCGCGCCTGAACCAGCTGGACCCGGAACTGATCGGTGTTGGACTGGTGAt                                                                                                                 >  2:789408/1‑237 (MQ=255)
                                                                                                                                        aaTTACCCTGCTGGGGCCGCGTCGTCTGGGGGCGCGTAACGTCGCGCAACGGCTGGCGGGGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAg                                                                                                                                   <  2:279043/195‑1 (MQ=255)
                                                                                                                                                  ctgGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGa                                                                                                                         >  1:279043/1‑195 (MQ=255)
                                                                                                                                                            gtcgtcTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGa                                                                                                                         <  2:55392/185‑1 (MQ=255)
                                                                                                                                                            gtcgtcTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGa                                                                                                                         >  1:55392/1‑185 (MQ=255)
                                                                                                                                                            gtcgtcTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCATGAGCGCAGCTTGCAGGCGGATttggcgtt                                                                    <  1:896803/238‑1 (MQ=255)
                                                                                                                                                               gttggggggggcgAAACGTCCCGAACGGGGTGGCGGAGCTTGTAAACGAAAGGCCAGGCGATCCCGTTGGCCACCGGGTGCGTGCGCAAAACTGCGTCGGGCCGAATCCCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCATGAGCGCAGCTTGCAGGCGGATttggcgttggcg                                                                <  2:885899/229‑1 (MQ=255)
                                                                                                                                                               gtcTGGCGGCGCGTAACGTCGCGCAGCGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACGGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCATGAGCGCAGCTTGCAGGCGGATttggcgttggcg                                                                <  1:980049/239‑1 (MQ=255)
                                                                                                                                                               gtcTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATCCCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCATGAGCGCAGCTTGCAGGCGGATttggcgttggcg                                                                <  1:513852/239‑1 (MQ=255)
                                                                                                                                                                        cgcgTAACGTCGCGCACCGGCTGGCGGCGCTGCTTAACGAAAGGCCAGGCGATACCGTGGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCATGAGCGCAGCTTGCAGGCGGATTTGGCGTTGGCGCTGTTACt                                                        <  2:88196/238‑1 (MQ=255)
                                                                                                                                                                        cgcgTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGTTCCTTGATGAATTTCATGAGCGCAGCTTGCTGGCGGATTTGGCGTTGGCGCGGTTACTc                                                       >  2:398779/1‑239 (MQ=255)
                                                                                                                                                                        agcgTACCGCCGCGCAACGGCTGGCGGGGCTGCTAAACGACAAGCCAGGCGATACCGTTGGCGCCCGGATGCGTGCGCAAACCTGCGTCGGGCCGAATCCCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCATGAGCGCAGCTTGCAGGCGGATTTGGCGTTGGCGCTGTTACt                                                        <  2:683950/237‑1 (MQ=255)
                                                                                                                                                                             aaCGGCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATCCCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCATGAGCGCAGCTTGCAGGCGGATTTGGCGTTGGCGCTGTTACTCGAtg                                                   <  1:141635/238‑1 (MQ=255)
                                                                                                                                                                                                       gctTAACGAAAAGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCATGAGCGCAGCTTGCAGGCGGATTTGGCGTTGGCGCTGTTACTCGATGTGCAACAAGGTCTGCGTGATGACCTTa                        <  1:1127502/239‑1 (MQ=255)
                                                                                                                                                                                                               aaaaGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCAt                                                                                                 >  1:160326/1‑158 (MQ=255)
                                                                                                                                                                                                               aaaaGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCAt                                                                                                 <  2:160326/158‑1 (MQ=255)
                                                                                                                                                                                                                  aGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCt                                                                                                                                                                    >  1:566604/1‑88 (MQ=255)
                                                                                                                                                                                                                  aGCCAGGCGATACCGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCt                                                                                                                                                                    <  2:566604/88‑1 (MQ=255)
                                                                                                                                                                                                                              ccGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCATGAGCGCAGCTTGCAGGCGGATTTGGCGTTGGCGCTGTTACTCGAtgt                                                  <  2:1177709/190‑1 (MQ=255)
                                                                                                                                                                                                                              ccGTTGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCATGAGCGCAGCTTGCAGGCGGATTTGGCGTTGGCGCTGTTACTCGAtgt                                                  >  1:1177709/1‑190 (MQ=255)
                                                                                                                                                                                                                                 ttGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCATGAGCGCAGCTTGCAGGCGGATTTGGCGTTTGCGCTGTTACTCGATGTGCAACAAGGTCTGCGTGATGACCTTTAACTGCTGACTATGTCGGCTAc  >  2:982996/1‑235 (MQ=255)
                                                                                                                                                                                                                                 ttGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCATGAGCGCAGCTTGCAGGCGGATTTGGCGTTGGCGCTGTTACTCg                                                      <  2:155781/183‑1 (MQ=255)
                                                                                                                                                                                                                                 ttGGCTCCCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCATGAGCGCAGCTTGCAGGCGGATTTGGCGTTGGCGCTGTTACTCg                                                      >  1:155781/1‑183 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                    
CGTTGCTGCCGTCTTACCTGAATTACTTACCGCTCTCGATTGTGCGCCGCAGGTATTATTAAGTGCGCCGACCGGGGCCGGGAAAT‑CAACCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTACCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCATGAGCGCAGCTTGCAGGCGGATTTGGCGTTGGCGCTGTTACTCGATGTGCAACAAGGTCTGCGTGATGACCTTAAACTGCTGATTATGTCGGCTAC  >  NC_000913/162119‑162577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A