Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I52 R1	239	220.4	11079197	93.2%	10325811	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	754,738	A→C	intergenic (‑270/‑439)	gltA ← / → sdhC	citrate synthase/succinate dehydrogenase, membrane subunit, binds cytochrome b556

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,738	0	A	C	97.7%	768.8 / ‑2.7	219	intergenic (‑270/‑439)	gltA/sdhC	citrate synthase/succinate dehydrogenase, membrane subunit, binds cytochrome b556
Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (106/107); total (111/108)
Fisher's exact test for biased strand distribution p-value = 5.98e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

AATGGCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTGTCAAA  >  NC_000913/754641‑754833

aaTGGCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAg                                                                                               <  2:247616/100‑1 (MQ=255)

 aTGGCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGt                                                                                              >  1:4142652/1‑100 (MQ=255)

 aTGGCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGt                                                                                              >  1:2151833/1‑100 (MQ=255)

   ggCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTa                                                                                            >  1:4602687/1‑100 (MQ=255)

      gAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACTACTAATAACTGTCCCGAATGAATTGGTCGATACTTCCACACTGTTACATCAGTTAATc                                                                                         <  1:5333740/100‑1 (MQ=255)

      gAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATc                                                                                         >  2:2813664/1‑100 (MQ=255)

      gAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATc                                                                                         >  2:569749/1‑100 (MQ=255)

        aTCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGCTCAATACTTCCACACTGTTACATCAGTTAATCtt                                                                                       <  1:1595867/100‑1 (MQ=255)

         tCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTa                                                                                      <  1:2078610/100‑1 (MQ=255)

          cAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAg                                                                                     <  2:290633/100‑1 (MQ=255)

           aaGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAgg                                                                                    <  1:3587868/100‑1 (MQ=255)

           aaGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAgg                                                                                    <  2:2408680/100‑1 (MQ=255)

            aGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGt                                                                                   <  2:3809312/100‑1 (MQ=255)

               aCTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGaa                                                                                >  2:2188499/1‑100 (MQ=255)

                  tAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATa                                                                             >  1:3739903/1‑100 (MQ=255)

                      aaTCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGa                                                                         >  1:1930007/1‑100 (MQ=255)

                      aaTCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGa                                                                         >  1:1450626/1‑100 (MQ=255)

                       aTCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGAc                                                                        <  1:3307902/100‑1 (MQ=255)

                        tCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACt                                                                       <  1:845768/100‑1 (MQ=255)

                           gAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCa                                                                    >  1:2365888/1‑100 (MQ=255)

                             attattAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATa                                                                  <  2:3280573/100‑1 (MQ=255)

                               tattaAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAAc                                                                >  2:4007330/1‑100 (MQ=255)

                               tattaAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAAc                                                                >  1:4193363/1‑100 (MQ=255)

                                attaAGCTTGTCCACCAATGATAACAGTCCCGGATCAATTGGTCAATACGTCCACACTGTTACATCAGTTAATGTTAGGTGAAATACCGACTCAATAACt                                                               <  2:536490/100‑1 (MQ=255)

                                    aaCTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTa                                                           <  2:1005175/100‑1 (MQ=255)

                                    aaCTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTa                                                           <  2:2084373/100‑1 (MQ=255)

                                     aCTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTAc                                                          <  1:3178163/100‑1 (MQ=255)

                                      cTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACg                                                         <  1:2048588/100‑1 (MQ=255)

                                      cTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACg                                                         <  1:230192/100‑1 (MQ=255)

                                      cTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACg                                                         <  1:5158196/100‑1 (MQ=255)

                                      cTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACg                                                         <  1:2750712/100‑1 (MQ=255)

                                      cTTGTATACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTGTACg                                                         >  1:4995432/1‑100 (MQ=255)

                                       ttGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGc                                                        >  2:501903/1‑100 (MQ=255)

                                         gTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCAt                                                      >  1:3656075/1‑100 (MQ=255)

                                           ctactactaAGAACTGTCCCGAATGGTTTTGTCAATACTTCCACACTGTGACCTATGTTAATCTTAGGACAAATACCGCCTTCATACCTTTTACGCATta                                                    >  2:5172129/1‑100 (MQ=255)

                                           cTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATta                                                    <  2:3256527/100‑1 (MQ=255)

                                            tACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATtat                                                   >  2:4431523/1‑100 (MQ=255)

                                            tACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATtat                                                   >  1:4086235/1‑100 (MQ=255)

                                              ccACTACTGACTGTCCCGAATGAATTTGTCAAGACTTCCACACTGTTCCATCAGTTCATCTTAGGTGAAATACCGACTTCATAACTTTTGGGCATtattt                                                 >  2:197163/1‑98 (MQ=255)

                                               cACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATg                                                >  2:1341566/1‑100 (MQ=255)

                                                aCTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGc                                               >  1:1282144/1‑100 (MQ=255)

                                                aCTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGc                                               >  2:2668865/1‑100 (MQ=255)

                                                   aataaCTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCttt                                            >  1:1608377/1‑100 (MQ=255)

                                                   aataaCTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCttt                                            <  1:1714193/100‑1 (MQ=255)

                                                     taaCTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTc                                          <  2:2739771/100‑1 (MQ=255)

                                                     taaCTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTc                                          <  2:1953730/100‑1 (MQ=255)

                                                       aCTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCt                                        <  2:5493651/100‑1 (MQ=255)

                                                        cTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTg                                       <  1:792683/100‑1 (MQ=255)

                                                          gTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGt                                     >  2:3851931/1‑100 (MQ=255)

                                                            cccGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTaa                                   >  2:1317702/1‑100 (MQ=255)

                                                            cccGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTaa                                   >  2:756780/1‑100 (MQ=255)

                                                            cccGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTaa                                   >  2:455001/1‑100 (MQ=255)

                                                            cccGAATGAATTGGTCAATACTTCCACACCGTTACATCAGTTAATCTTAGGTGAAATACCGACTTAATAACTTTTACGCATTATATGCTTTTCCTGGTaa                                   <  1:5297248/100‑1 (MQ=255)

                                                             ccGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAAt                                  <  2:3704828/100‑1 (MQ=255)

                                                              cGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATg                                 >  1:3430428/1‑100 (MQ=255)

                                                               gaatgaatTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGt                                >  2:3466326/1‑100 (MQ=255)

                                                               gaatgaatTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGt                                >  1:4641560/1‑100 (MQ=255)

                                                                aatgaatTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATAtt                               <  2:4128852/100‑1 (MQ=255)

                                                                 atgaatTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGttt                              >  1:1897264/1‑100 (MQ=255)

                                                                   gaatTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGt                            <  1:2279895/100‑1 (MQ=255)

                                                                   gaatTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGt                            <  1:2840711/100‑1 (MQ=255)

                                                                    aatTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTa                           <  2:1265172/100‑1 (MQ=255)

                                                                    aatTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTa                           <  2:4587228/100‑1 (MQ=255)

                                                                    aatTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTa                           <  1:1133768/100‑1 (MQ=255)

                                                                    aatTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTa                           >  1:4033657/1‑100 (MQ=255)

                                                                    aatTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTa                           <  2:1730519/100‑1 (MQ=255)

                                                                    aatTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTa                           >  2:1546226/1‑100 (MQ=255)

                                                                    aatTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTa                           >  1:4328769/1‑100 (MQ=255)

                                                                     atTGGGCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTaa                          >  2:66889/1‑100 (MQ=255)

                                                                       tGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTaaca                        <  1:576507/100‑1 (MQ=255)

                                                                        ggTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTaacaa                       >  1:2193839/1‑100 (MQ=255)

                                                                         gTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTaacaac                      >  1:1018086/1‑100 (MQ=255)

                                                                         gTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTaacaac                      >  2:3225550/1‑100 (MQ=255)

                                                                          tCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACt                     >  1:2249944/1‑100 (MQ=255)

                                                                          tCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACt                     >  1:2708763/1‑100 (MQ=255)

                                                                           cAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGTAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACtt                    <  2:2314730/100‑1 (MQ=255)

                                                                           cAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACtt                    >  1:4739955/1‑100 (MQ=255)

                                                                           cAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAAGACAGACTTCATAACTTTTACGAATTATACGATTTTCCTGGTAATGTTTGTAACAAAtt                    >  2:5228201/1‑100 (MQ=255)

                                                                              tACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTttgt                 <  2:3032421/100‑1 (MQ=255)

                                                                                cTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTttgttg               <  1:3894821/100‑1 (MQ=255)

                                                                                cTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTttgttg               <  2:47389/100‑1 (MQ=255)

                                                                                cTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTttgttg               <  2:3393921/100‑1 (MQ=255)

                                                                                cTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTttgttg               >  1:48011/1‑100 (MQ=255)

                                                                                cTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTCTGTAACAACTttgttg               >  1:617766/1‑100 (MQ=255)

                                                                                 ttCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGa              <  1:5460520/100‑1 (MQ=255)

                                                                                  tCCACCCTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTAATAACTTTTACGCATTATATGCATTTCATGCTAATGTTTGTAACCACTTTGTTGaa             >  2:4832501/1‑100 (MQ=255)

                                                                                  tCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGaa             >  2:3736751/1‑100 (MQ=255)

                                                                                   ccACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAAt            <  2:1180622/100‑1 (MQ=255)

                                                                                    cacaCTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATg           <  2:4896167/100‑1 (MQ=255)

                                                                                    cacaCTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATg           <  2:2155464/100‑1 (MQ=255)

                                                                                     acacTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGa          <  2:3325232/100‑1 (MQ=255)

                                                                                      cacTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGAt         <  2:1484837/100‑1 (MQ=255)

                                                                                       acTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGAtt        <  2:4261186/100‑1 (MQ=255)

                                                                                        cTGTTACATCAGTTAATCTTAGGTGAAATAACGAATTCATAACTTTTACGGATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGAtag       <  2:1040147/100‑3 (MQ=255)

                                                                                          gTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTGTc     <  1:5092190/100‑1 (MQ=255)

                                                                                          gTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTGTc     >  1:4186364/1‑100 (MQ=255)

                                                                                           ttACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTGTCa    <  2:5410542/100‑1 (MQ=255)

                                                                                           ttACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTGTCa    <  2:610396/100‑1 (MQ=255)

                                                                                            tACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTGTCaa   >  2:3407170/1‑100 (MQ=255)

                                                                                            tACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTGTCaa   >  2:2241402/1‑100 (MQ=255)

AATGGCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTGTCAAA  >  NC_000913/754641‑754833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A