Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I53 R1	229	158.0	8090706	91.1%	7370633	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	754,738	A→C	intergenic (‑270/‑439)	gltA ← / → sdhC	citrate synthase/succinate dehydrogenase, membrane subunit, binds cytochrome b556

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,738	0	A	C	96.3%	439.0 / 2.8	134	intergenic (‑270/‑439)	gltA/sdhC	citrate synthase/succinate dehydrogenase, membrane subunit, binds cytochrome b556
Reads supporting (aligned to +/- strand): ref base A (3/2); new base C (60/69); total (63/71)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 135 total aligned reads displayed.

AATGGCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTGTCAAAT  >  NC_000913/754641‑754834

aaTGGCGAATCAAGTACTTAGCAATGCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCAAATGCATTGGTAAATACTTCCACACTGTTACATacg                                                                                                >  2:22823/1‑98 (MQ=255)

 aTGGCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGt                                                                                               >  1:2638757/1‑100 (MQ=255)

 aTGGCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGt                                                                                               >  2:2437930/1‑100 (MQ=255)

  tGGCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGtt                                                                                              <  2:1451822/100‑1 (MQ=255)

  tGGCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAAGTGGTCAATACTTCCACACTGTTACATCAGtt                                                                                              <  1:1375909/100‑1 (MQ=255)

   ggCGAATCAAGTACTTAACAATCCGAATTATTAAACTTGTCTACCAGTAGTAACTGTCCCGAATGAATTGGTAAATACTTCCACACTGTTACATCAGTTa                                                                                             >  2:429749/1‑100 (MQ=255)

    gCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTaa                                                                                            <  1:146707/100‑1 (MQ=255)

     cGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAAt                                                                                           <  2:1663727/100‑1 (MQ=255)

     cGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAAt                                                                                           >  1:3913030/1‑100 (MQ=255)

      gAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATc                                                                                          >  2:1801709/1‑100 (MQ=255)

      gAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATc                                                                                          <  2:2584952/100‑1 (MQ=255)

      gAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATc                                                                                          <  1:339323/100‑1 (MQ=255)

      gAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATc                                                                                          >  1:3050427/1‑100 (MQ=255)

      gAATCAAGTACTTAGCAATCCGAATTAGTAATCTTGTCTCTCACTAATAGCTGTCCCGAATGACTTGGTCAATACTGCCACACTTTTACATCAGTTAATc                                                                                          <  2:924673/100‑1 (MQ=255)

       aaTCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCt                                                                                         <  1:1739006/100‑1 (MQ=255)

         tCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTa                                                                                       <  2:1402582/100‑1 (MQ=255)

          cAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAg                                                                                      <  1:1417202/100‑1 (MQ=255)

          cAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAg                                                                                      <  1:2307735/100‑1 (MQ=255)

           aaGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAgg                                                                                     <  1:3040656/100‑1 (MQ=255)

            aGTACTTAGCAATCCGAAGTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGt                                                                                    <  1:1391895/100‑1 (MQ=255)

               aCTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGaa                                                                                 >  2:681919/1‑100 (MQ=255)

                cTTAGCAATCCGAATTATTAAACTTGTCTACCGATAATGACTGTCCAGAATGAATTGGTCAATACTTCCACACTGTTACAGCAGTTAATCTTAGGTGaaa                                                                                >  1:3626437/1‑100 (MQ=255)

                cTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGaaa                                                                                >  1:920682/1‑100 (MQ=255)

                cTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGaaa                                                                                >  1:475566/1‑100 (MQ=255)

                 ttAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAAt                                                                               <  2:1005239/100‑1 (MQ=255)

                    gCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATAcc                                                                            <  2:2875189/100‑1 (MQ=255)

                    gCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATAcc                                                                            <  2:2648358/100‑1 (MQ=255)

                    gCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATAcc                                                                            <  1:3512349/100‑1 (MQ=255)

                     cAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCg                                                                           <  2:3922302/100‑1 (MQ=255)

                     cAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCg                                                                           >  1:3326486/1‑100 (MQ=255)

                     cAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCg                                                                           <  2:2772646/100‑1 (MQ=255)

                      aaTCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGa                                                                          >  2:442535/1‑100 (MQ=255)

                      aaTCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGa                                                                          >  2:2760566/1‑100 (MQ=255)

                       aTCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGAc                                                                         >  2:3242975/1‑100 (MQ=255)

                       aTCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGAc                                                                         >  2:1264962/1‑100 (MQ=255)

                         ccGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACtt                                                                       <  1:535718/100‑1 (MQ=255)

                          cGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTc                                                                      >  1:935530/1‑100 (MQ=255)

                               tattaAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAAc                                                                 <  2:314830/100‑1 (MQ=255)

                                 ttaAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACtt                                                               <  1:968686/100‑1 (MQ=255)

                                 ttaAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACtt                                                               >  2:2707923/1‑100 (MQ=255)

                                   aaaCTTGTCTACCACTAATAACTGTCCCGAACGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACtttt                                                             >  1:3271401/1‑100 (MQ=255)

                                    aaCTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTa                                                            <  2:2854323/100‑1 (MQ=255)

                                    aaCTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTa                                                            <  1:440766/100‑1 (MQ=255)

                                    aaCTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTa                                                            <  1:1435941/100‑1 (MQ=255)

                                    aaCTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTa                                                            <  2:51444/100‑1 (MQ=255)

                                     aCTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTAc                                                           <  1:2454134/100‑1 (MQ=255)

                                      cTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACg                                                          <  2:499141/100‑1 (MQ=255)

                                         gTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCAt                                                       <  2:1146773/100‑1 (MQ=255)

                                         gTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCAt                                                       <  1:4011920/100‑1 (MQ=255)

                                         gTCTACCACTAATAACTGCCTCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCAt                                                       <  1:3821353/100‑1 (MQ=255)

                                          tCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCAtt                                                      <  2:1139548/100‑1 (MQ=255)

                                          tCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCAtt                                                      >  2:3844383/1‑100 (MQ=255)

                                            tACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATtat                                                    >  1:2895488/1‑100 (MQ=255)

                                              ccACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTatat                                                  >  2:2980793/1‑100 (MQ=255)

                                              ccACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTatat                                                  >  2:1419405/1‑100 (MQ=255)

                                               cACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATg                                                 <  2:45242/100‑1 (MQ=255)

                                                aCTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGc                                                <  1:1288693/100‑1 (MQ=255)

                                                aCTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGc                                                >  1:3852086/1‑100 (MQ=255)

                                                 cTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCt                                               >  1:2891092/1‑100 (MQ=255)

                                                 cTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCt                                               <  2:2294774/100‑1 (MQ=255)

                                                       aCTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAGCTTTTACGCATTATATGCTTTTCCt                                         <  1:3642222/100‑1 (MQ=255)

                                                       aCTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCt                                         <  1:2114993/100‑1 (MQ=255)

                                                        cTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTg                                        <  1:2147242/100‑1 (MQ=255)

                                                        cTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTg                                        >  1:2220176/1‑100 (MQ=255)

                                                        cTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTg                                        >  1:2929439/1‑100 (MQ=255)

                                                        cTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTg                                        <  2:3771132/100‑1 (MQ=255)

                                                         tGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTgg                                       <  2:651064/100‑1 (MQ=255)

                                                          gTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGt                                      <  1:2979377/100‑1 (MQ=255)

                                                          gTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGt                                      <  1:3211504/100‑1 (MQ=255)

                                                            cccGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTaa                                    >  1:265022/1‑100 (MQ=255)

                                                             ccGAATGAATTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAAt                                   >  2:2784988/1‑100 (MQ=255)

                                                                  tgaatTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTg                              <  2:894339/100‑1 (MQ=255)

                                                                    aatTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTa                            <  2:2753756/100‑1 (MQ=255)

                                                                    aatTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTa                            <  2:1210864/100‑1 (MQ=255)

                                                                    aatTGGTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTa                            <  1:2252008/100‑1 (MQ=255)

                                                                        tctCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTaacaa                        <  1:1169910/98‑1 (MQ=255)

                                                                        ggTCAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTaacaa                        >  1:4065853/1‑100 (MQ=255)

                                                                           cAATACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTaacaaaga                     >  2:1147242/1‑97 (MQ=255)

                                                                            aaTACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACttt                    >  2:2593542/1‑100 (MQ=255)

                                                                            aaTACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACttt                    >  1:169336/1‑100 (MQ=255)

                                                                             aTACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTttg                   >  1:3576905/1‑100 (MQ=255)

                                                                              tACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTttgt                  <  2:1274278/100‑1 (MQ=255)

                                                                              tACTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTttgt                  <  2:1611530/100‑1 (MQ=255)

                                                                               aCTTCCACACTGTTACATCAGTTAATCTTTGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTttgtt                 <  2:704313/100‑1 (MQ=255)

                                                                                cTTCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTttgttg                >  2:1762775/1‑100 (MQ=255)

                                                                                  tCCACCCTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGCTGaa              >  1:3661623/1‑100 (MQ=255)

                                                                                  tCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGaa              <  2:1902286/100‑1 (MQ=255)

                                                                                  tCCACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGaa              <  2:444899/100‑1 (MQ=255)

                                                                                   ccACACTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAAt             <  1:2578921/100‑1 (MQ=255)

                                                                                        cTGTTACATCAGTTAATCTTAGGTGTATTACCGATTTCATAGCTTTTACGCATTATATGCTTTGCCTGGGAATGTTTGTAACAACTTTGTTGAATGATTg        <  2:495945/100‑1 (MQ=255)

                                                                                        cTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTg        >  2:442571/1‑100 (MQ=255)

                                                                                        cTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTg        <  1:832823/100‑1 (MQ=255)

                                                                                        cTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTg        >  2:719408/1‑100 (MQ=255)

                                                                                        cTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTg        <  2:767489/100‑1 (MQ=255)

                                                                                        cTGTTACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAACGTTTGTAACAACTTTGTTGAATGATTg        <  2:3982158/100‑1 (MQ=255)

                                                                                           ttACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTGTCa     <  1:2145010/100‑1 (MQ=255)

                                                                                           ttACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTGTCa     >  1:2233997/1‑100 (MQ=255)

                                                                                           ttACATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTGTCa     >  2:2585040/1‑100 (MQ=255)

                                                                                              cATCAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTGTCAAAt  >  1:976225/1‑100 (MQ=255)

                                                                                              cATCAGTTAAGCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGATTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTGTCAAAt  >  2:3685242/1‑100 (MQ=255)

AATGGCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTGTCAAAT  >  NC_000913/754641‑754834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A