Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 846,198 A→C F89C (TTC→TGC)  glnQ ← glutamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913846,1980AC98.1% 737.4 / ‑0.8 210F89C (TTC→TGC) glnQglutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base C (106/99);  total (109/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 210 total aligned reads displayed.

CAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCAGAT  >  NC_000913/846101‑846294
                                                                                                 |                                                                                                
cagcagCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCag                                                                                                <  2:1330824/100‑1 (MQ=255)
 agcagcTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCaga                                                                                               >  2:4431250/1‑100 (MQ=255)
   cagcTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAgg                                                                                             <  1:2943015/100‑1 (MQ=255)
   cagcTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCACGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAgg                                                                                             <  1:395986/100‑1 (MQ=255)
     gcTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTa                                                                                           <  1:546583/100‑1 (MQ=255)
     gcTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTa                                                                                           >  1:3099590/1‑100 (MQ=255)
     gcTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTa                                                                                           >  1:4883889/1‑100 (MQ=255)
      cTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTaa                                                                                          >  1:2469801/1‑100 (MQ=255)
       tCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTaaa                                                                                         >  1:1169989/1‑100 (MQ=255)
        cACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTaaaa                                                                                        >  1:5368741/1‑100 (MQ=255)
         aCGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGTGCAGAGGTAAAAc                                                                                       <  2:2595218/100‑1 (MQ=255)
            tGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAActgn                                                                                    <  1:4450557/100‑2 (MQ=255)
            ngCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAActgc                                                                                    >  1:4448605/2‑100 (MQ=255)
              ccAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAActgctg                                                                                  >  1:4166407/1‑100 (MQ=255)
              ccAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAActgctg                                                                                  >  2:1709393/1‑100 (MQ=255)
              ccAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAActgctg                                                                                  <  1:1429260/100‑1 (MQ=255)
               cAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTgg                                                                                 >  2:3316202/1‑100 (MQ=255)
               cAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGATgg                                                                                 >  1:2784766/1‑100 (MQ=255)
                aGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGa                                                                                >  1:1975168/1‑100 (MQ=255)
                 gTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGaa                                                                               <  2:4775648/100‑1 (MQ=255)
                  tttttCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAac                                                                              <  1:1473106/100‑1 (MQ=255)
                     ttCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAGACATGACGTTTGCCAGCGCGGTCAGATGCCGGCAGAGGTAAAACTGCTGGAACAcc                                                                           <  2:2085126/100‑1 (MQ=255)
                     ttCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACAcc                                                                           <  1:3347355/100‑1 (MQ=255)
                       ccGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCAt                                                                         >  2:4013379/1‑100 (MQ=255)
                       ccGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCAt                                                                         <  2:748388/100‑1 (MQ=255)
                       ccGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCAt                                                                         >  2:2037597/1‑100 (MQ=255)
                       ccGCCTCATCATTGTTCGCACCACGCCCGCGTAACAGGCCAAACATGACGTTTTCCAGCGCTGTCAAATGCGGGCAGAGGTAAACCTGCTAGAACACCAt                                                                         >  2:2449144/1‑100 (MQ=255)
                        cGCCTCTTCTTTTTTCGCGGCACGCATGCGTGGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATa                                                                        <  2:3007384/100‑1 (MQ=255)
                        cGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATa                                                                        >  2:1881089/1‑100 (MQ=255)
                        cGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATa                                                                        <  1:2793659/100‑1 (MQ=255)
                        cGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATa                                                                        >  2:1611914/1‑100 (MQ=255)
                        cGCCTCTTCTTTGTACGCGCCACGCAGGCGTAGCGGGCCAAACATGACGTTTTCGAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCCTa                                                                        >  2:5144594/1‑100 (MQ=255)
                         gCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATAc                                                                       >  1:5149506/1‑100 (MQ=255)
                         gCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATAc                                                                       >  1:1744616/1‑100 (MQ=255)
                           ctctTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCt                                                                     >  1:126425/1‑100 (MQ=255)
                           ctctTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCt                                                                     >  1:2330350/1‑100 (MQ=255)
                            tcttctTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTg                                                                    <  1:4848448/100‑1 (MQ=255)
                            tcttctTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTg                                                                    <  2:4254042/100‑1 (MQ=255)
                            tcttctTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTg                                                                    >  2:78348/1‑100 (MQ=255)
                             cttcttTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGc                                                                   >  2:1026214/1‑100 (MQ=255)
                             cttcttTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGc                                                                   >  1:3637346/1‑100 (MQ=255)
                               tcttTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCtt                                                                 >  2:3727585/1‑100 (MQ=255)
                               tcttTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCtt                                                                 <  2:816773/100‑1 (MQ=255)
                               tcttTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCtt                                                                 >  1:3187304/1‑100 (MQ=255)
                                cttTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTc                                                                >  2:1848730/1‑100 (MQ=255)
                                 tttGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTcc                                                               >  1:2841690/1‑100 (MQ=255)
                                 tttGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGACAGAGGTAAAACTGCTGGAACACCATACCTGCTTcc                                                               >  2:230378/1‑100 (MQ=255)
                                    gTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTgg                                                            <  1:1647678/100‑1 (MQ=255)
                                     ttCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGc                                                           >  2:4784318/1‑100 (MQ=255)
                                        gcgcCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGaa                                                        <  2:4699746/100‑1 (MQ=255)
                                         cgcCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAAt                                                       >  2:5457051/1‑100 (MQ=255)
                                          gcCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATc                                                      <  1:1998340/100‑1 (MQ=255)
                                          gcCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATc                                                      <  1:3546935/100‑1 (MQ=255)
                                          gcCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATc                                                      <  1:4105831/100‑1 (MQ=255)
                                          gcCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACAATACCTGCTTCCTGGCGAATc                                                      <  1:4327542/100‑1 (MQ=255)
                                           ccACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCa                                                     <  1:2625283/100‑1 (MQ=255)
                                              cgcacgcGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGgc                                                  >  2:1728781/1‑100 (MQ=255)
                                              cgcacgcGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGgc                                                  >  1:3302457/1‑100 (MQ=255)
                                               gcacgcGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGgcg                                                 >  1:4393236/1‑100 (MQ=255)
                                                cacgcGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGgcgc                                                >  1:4308747/1‑100 (MQ=255)
                                                 acgcGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCt                                               <  1:708283/100‑1 (MQ=255)
                                                 acgcGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCt                                               <  2:5077228/100‑1 (MQ=255)
                                                   gcgTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCg                                             <  2:3070785/100‑1 (MQ=255)
                                                            gCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTc                                    <  2:3210343/100‑1 (MQ=255)
                                                            gCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTc                                    <  2:3550150/100‑1 (MQ=255)
                                                            gCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTc                                    >  2:4546189/1‑100 (MQ=255)
                                                             ccAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCg                                   >  2:10451/1‑100 (MQ=255)
                                                             ccAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCg                                   <  2:523699/100‑1 (MQ=255)
                                                             acAAACTTGCCGCCTTCGATCGCTGTCAGCGGCGGGAAGAGGTAAAACTGGCGAACCACCTTACTGGCTTCCTTGCGGATCAGGCGCTCGTTAACTTTCg                                   <  2:3440704/99‑1 (MQ=255)
                                                              cAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCgg                                  <  1:3224724/100‑1 (MQ=255)
                                                                  cATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCg                              <  1:4688174/100‑1 (MQ=255)
                                                                   aTGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGt                             >  1:2215832/1‑100 (MQ=255)
                                                                     gACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTa                           >  1:4561099/1‑100 (MQ=255)
                                                                     gACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTa                           <  2:3929466/100‑1 (MQ=255)
                                                                        gTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAAcc                        <  1:232645/100‑1 (MQ=255)
                                                                        gTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAAcc                        <  2:4367321/100‑1 (MQ=255)
                                                                           ttCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTc                     <  1:4904043/100‑1 (MQ=255)
                                                                            tCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCa                    >  2:2961076/1‑100 (MQ=255)
                                                                            tCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCa                    <  1:63721/100‑1 (MQ=255)
                                                                             ccAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAg                   <  1:1933507/100‑1 (MQ=255)
                                                                             ccAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAg                   <  1:1039988/100‑1 (MQ=255)
                                                                             ccAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAg                   <  2:3361964/100‑1 (MQ=255)
                                                                              cAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGCTCGTTAACCTTCAgg                  <  2:6975/100‑1 (MQ=255)
                                                                              cAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAgg                  >  1:5093255/1‑100 (MQ=255)
                                                                              cAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAgg                  <  1:2047851/100‑1 (MQ=255)
                                                                              cAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAgg                  <  2:5316136/100‑1 (MQ=255)
                                                                               aGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGc                 <  1:4014870/100‑1 (MQ=255)
                                                                                gcgcTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGcc                >  1:123626/1‑100 (MQ=255)
                                                                                   cTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATc             <  1:592292/100‑1 (MQ=255)
                                                                                    tGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCg            <  1:1849983/100‑1 (MQ=255)
                                                                                     gTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGa           <  2:1945779/100‑1 (MQ=255)
                                                                                      tCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGAc          <  1:1125781/100‑1 (MQ=255)
                                                                                      tCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGAc          <  1:4243941/100‑1 (MQ=255)
                                                                                       cAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACa         >  1:7755/1‑100 (MQ=255)
                                                                                       cAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACa         >  1:924149/1‑100 (MQ=255)
                                                                                         gATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAAt       <  1:1729426/100‑1 (MQ=255)
                                                                                         gATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAAt       >  2:4481748/1‑100 (MQ=255)
                                                                                           tGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCa     <  2:629112/100‑1 (MQ=255)
                                                                                           tGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCa     >  1:3701021/1‑100 (MQ=255)
                                                                                            gCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCAg    <  1:4794586/100‑1 (MQ=255)
                                                                                                 |                                                                                                
CAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCAGAT  >  NC_000913/846101‑846294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A