Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 846,198 A→C F89C (TTC→TGC)  glnQ ← glutamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913846,1980AC99.4% 551.5 / ‑4.3 157F89C (TTC→TGC) glnQglutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (66/88);  total (68/89)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

Only 100 of 157 total aligned reads displayed.

CAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCAGAT  >  NC_000913/846101‑846294
                                                                                                 |                                                                                                
  gcagcTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCagag                                                                                              >  1:1490895/1‑100 (MQ=255)
  gcagcTCACGTGCCAGTTTTTCCGCCTCTGCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCagag                                                                                              <  2:2418750/100‑1 (MQ=255)
   ccgcTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAgg                                                                                             <  2:2268495/98‑1 (MQ=255)
   cagcTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAgg                                                                                             <  2:2769411/100‑1 (MQ=255)
   cagcTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAgg                                                                                             >  1:2732612/1‑100 (MQ=255)
     gcTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTa                                                                                           >  2:876514/1‑100 (MQ=255)
       tCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTaaa                                                                                         <  1:3741909/100‑1 (MQ=255)
       tCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTaaa                                                                                         <  1:931440/100‑1 (MQ=255)
        cACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTaaaa                                                                                        >  2:3824568/1‑100 (MQ=255)
         aCGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAAc                                                                                       <  1:3501789/100‑1 (MQ=255)
            tGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAActgc                                                                                    >  1:1752756/1‑100 (MQ=255)
            tGCCAGGTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCTGTCCAAACATGACGGTTTCCAGCGCTGCCAGATGCGGGCAGAGGTACAAcggc                                                                                    >  2:2196219/1‑100 (MQ=255)
             gCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAActgct                                                                                   >  2:1902761/1‑100 (MQ=255)
             ctcAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAActgct                                                                                   <  1:2887839/98‑1 (MQ=255)
              ccAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAActgctg                                                                                  <  2:2098968/100‑1 (MQ=255)
              ccAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAActgctg                                                                                  <  2:1616744/100‑1 (MQ=255)
              ccAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAActgctg                                                                                  >  1:3464755/1‑100 (MQ=255)
              ccAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAActgctg                                                                                  <  1:1118622/100‑1 (MQ=255)
              ccAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAActgctg                                                                                  <  1:3648605/100‑1 (MQ=255)
               cAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAActgctag                                                                                 >  2:2844407/1‑98 (MQ=255)
                  tttttCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAac                                                                              <  2:2010367/100‑1 (MQ=255)
                  tttttCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAac                                                                              <  2:1100330/100‑1 (MQ=255)
                  tttttCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAac                                                                              <  2:1934738/100‑1 (MQ=255)
                     ttCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACAcc                                                                           <  1:267422/100‑1 (MQ=255)
                      tCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCa                                                                          >  1:727011/1‑100 (MQ=255)
                      tCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCa                                                                          >  1:1951164/1‑100 (MQ=255)
                      tCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGGAAAACTGCTGGAACACCa                                                                          >  2:3336989/1‑100 (MQ=255)
                        cGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGCTGCGGGCAGAGGTAAAACTGCTGGAACACCATa                                                                        >  2:1534861/1‑100 (MQ=255)
                        cGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATa                                                                        >  2:2225922/1‑100 (MQ=255)
                        cGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATa                                                                        >  1:3083639/1‑100 (MQ=255)
                         gCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATAc                                                                       <  2:550959/100‑1 (MQ=255)
                         gCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATAc                                                                       >  2:3103547/1‑100 (MQ=255)
                           ctctTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCt                                                                     <  2:1114303/100‑1 (MQ=255)
                              ttcttTGTTCGCGCCACGCACGCGTAGCGGGCCTAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCt                                                                  <  1:4041850/100‑1 (MQ=255)
                              ttcttTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCt                                                                  <  1:2346712/100‑1 (MQ=255)
                                cttTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTc                                                                >  1:509095/1‑100 (MQ=255)
                                cttTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTc                                                                >  1:1942472/1‑100 (MQ=255)
                                  ttGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCt                                                              <  2:2424129/100‑1 (MQ=255)
                                  ttGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCt                                                              <  1:21509/100‑1 (MQ=255)
                                   tGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTg                                                             <  1:1006781/100‑1 (MQ=255)
                                    gTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTgg                                                            >  2:2779838/1‑100 (MQ=255)
                                    gTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTgg                                                            >  1:15899/1‑100 (MQ=255)
                                    gTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTgg                                                            >  2:1278311/1‑100 (MQ=255)
                                    gTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTgg                                                            >  1:2888116/1‑100 (MQ=255)
                                     ttCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGc                                                           <  1:2586731/100‑1 (MQ=255)
                                     ttCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGc                                                           <  1:1007102/100‑1 (MQ=255)
                                       cgcgCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGa                                                         >  1:2115130/1‑100 (MQ=255)
                                        gcgcCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGan                                                        <  1:562494/100‑2 (MQ=255)
                                          gcCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATc                                                      >  1:1647082/1‑100 (MQ=255)
                                          gcCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATc                                                      >  1:1339341/1‑100 (MQ=255)
                                          gcCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATc                                                      <  2:313601/100‑1 (MQ=255)
                                           ccACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCa                                                     <  2:2692564/100‑1 (MQ=255)
                                             acgcacgcGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAgg                                                   <  1:2052387/100‑1 (MQ=255)
                                              cgcacgcGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGgc                                                  <  1:3203455/100‑1 (MQ=255)
                                              cgcacgcGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGgc                                                  <  2:1911646/100‑1 (MQ=255)
                                              cgcacgcGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGgc                                                  >  2:828778/1‑100 (MQ=255)
                                              cgcacgcGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGgc                                                  <  1:3919208/100‑1 (MQ=255)
                                              cgcacgcGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGgc                                                  <  2:3547426/100‑1 (MQ=255)
                                                cacgcGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGgcgc                                                >  1:2842897/1‑100 (MQ=255)
                                                  cgcgTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTc                                              >  2:439982/1‑100 (MQ=255)
                                                    cgTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGt                                            <  1:3559241/100‑1 (MQ=255)
                                                      tAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCa                                          >  2:2825702/1‑100 (MQ=255)
                                                        gCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAAc                                        <  1:846597/100‑1 (MQ=255)
                                                        gCGGGCCAAACATGACGTATTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAAc                                        <  1:2719360/100‑1 (MQ=255)
                                                         cGGTCCAAACATGACGTTTTCCAGCGCTGGCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACt                                       <  1:1330194/100‑1 (MQ=255)
                                                            gCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTc                                    >  1:2473926/1‑100 (MQ=255)
                                                            gCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTc                                    <  1:155261/100‑1 (MQ=255)
                                                            gCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTc                                    >  2:1221098/1‑100 (MQ=255)
                                                             ccAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCg                                   >  2:2895623/1‑100 (MQ=255)
                                                             ccAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCg                                   >  1:224266/1‑100 (MQ=255)
                                                                aaCATGACGTTTTCCAGCGCTTTCAGATGCGGGCAGAGGTAAAACTGCGGGAACACCATACCTGCTTCCTGGCAAATAAGGCGCTCGTCAACTCTCGGAt                                >  2:3221467/1‑100 (MQ=255)
                                                                  cATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCg                              >  1:2073233/1‑100 (MQ=255)
                                                                   aTGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGt                             >  2:1298214/1‑100 (MQ=255)
                                                                    tGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGtt                            >  1:619998/1‑100 (MQ=255)
                                                                    ngACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGtt                            >  1:1717775/2‑100 (MQ=255)
                                                                     gACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTa                           >  1:2753048/1‑100 (MQ=255)
                                                                     gACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTa                           >  1:2123310/1‑100 (MQ=255)
                                                                     gACGTTGTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTa                           >  1:2123190/1‑100 (MQ=255)
                                                                       cGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAAc                         <  2:2275268/100‑1 (MQ=255)
                                                                        gTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAAcc                        <  2:1038502/100‑1 (MQ=255)
                                                                        gTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAAcc                        <  1:1782436/100‑1 (MQ=255)
                                                                        gTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAAcc                        >  2:440490/1‑100 (MQ=255)
                                                                            tCCAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCa                    <  2:3494597/100‑1 (MQ=255)
                                                                             ccAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAg                   >  1:4001000/1‑100 (MQ=255)
                                                                             ccAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAg                   >  2:198609/1‑100 (MQ=255)
                                                                              cAGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAgg                  <  2:1670070/100‑1 (MQ=255)
                                                                               aGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGc                 <  1:1244388/100‑1 (MQ=255)
                                                                               aGCGCTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGc                 <  2:637779/100‑1 (MQ=255)
                                                                                gcgcTGTCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGcc                <  1:3618960/100‑1 (MQ=255)
                                                                                  gcTGTCAGATGCGGTCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCAt              <  2:3650236/100‑1 (MQ=255)
                                                                                      tCAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGAc          <  1:468281/100‑1 (MQ=255)
                                                                                       cAGATGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACa         >  2:3194253/1‑100 (MQ=255)
                                                                                          aTGCTGGCAGAGGTAAAACTGCTGCAACACCATACCGGCTTCCTGGCGAATCAGGCGCTCGGCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATc      <  1:3345840/100‑1 (MQ=255)
                                                                                          aTGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATc      <  2:833708/100‑1 (MQ=255)
                                                                                           tGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTTACCTTCAGGCCATCGACAATCa     <  1:3415707/100‑1 (MQ=255)
                                                                                           tGCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTGCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCa     <  2:2892795/100‑1 (MQ=255)
                                                                                           tGCGGGCAGAGGTAAAACTGCTCGAACACCATACCTGCTTCCTGGCAAATCAGGCGCGCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCa     <  1:3712314/100‑1 (MQ=255)
                                                                                            gCGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCGATCGACGATCAg    <  2:3844074/100‑1 (MQ=255)
                                                                                             cGGGCAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCAGa   >  1:753145/1‑100 (MQ=255)
                                                                                              gggCAGAGGTAAAACTGCTCGCAGACCATACGTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCAGAt  <  2:1810211/100‑1 (MQ=255)
                                                                                                 |                                                                                                
CAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCAGAT  >  NC_000913/846101‑846294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A