Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,035,960 T→G L64R (CTC→CGC)  hyaD → hydrogenase 1 maturation protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,035,9600TG98.9% 645.2 / ‑2.5 181L64R (CTC→CGC) hyaDhydrogenase 1 maturation protease
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (100/79);  total (101/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 182 total aligned reads displayed.

CATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGC  >  NC_000913/1035863‑1036055
                                                                                                 |                                                                                               
cATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTcgcg                                                                                               <  2:616520/100‑1 (MQ=255)
 aTTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGa                                                                                              >  1:1053565/1‑100 (MQ=255)
 aTTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGa                                                                                              >  1:4195649/1‑100 (MQ=255)
 aTTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGa                                                                                              >  2:5379394/1‑100 (MQ=255)
   tACCACTGGCCCGAGTATGTGGAGCTTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATg                                                                                            <  1:1851425/100‑1 (MQ=255)
    aCCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGTCTGAACTTGCTGGGGTATGTCGCGAGCGCCAGCCATCTGGTGATTCGCGATGc                                                                                           >  2:4290879/1‑100 (MQ=255)
    aCCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGc                                                                                           >  2:4068968/1‑100 (MQ=255)
       aCTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCAt                                                                                        <  1:2120242/100‑1 (MQ=255)
       aCTGGCCCGAGTATGTGGAGATTGTCGATGGAGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCAt                                                                                        >  2:2292815/1‑100 (MQ=255)
         tGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTg                                                                                      <  1:1990022/100‑1 (MQ=255)
         tGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTg                                                                                      <  2:5011270/100‑1 (MQ=255)
         tGGCCCCGGTATGTGGAGAGTGTCGATGGGGGTACTCAGCGACTGACGTTGCTTTGGTATGTCGAAAGCGCCAGCCATCTCTTGCTTCGCGATGCCATTg                                                                                      <  2:2402129/100‑1 (MQ=255)
          ggCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGa                                                                                     >  1:639496/1‑100 (MQ=255)
            cccGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACt                                                                                   <  1:2760475/100‑1 (MQ=255)
            cccGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACt                                                                                   >  1:738101/1‑100 (MQ=255)
            cccGAGTATGTGGAGATGGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACt                                                                                   <  2:4734888/100‑1 (MQ=255)
                  tATGTGGAGATTGTCAATGGCGGTACTCAGAGACTGAACCTGCTGAGGTATGTCGAAGGCGCCAGCCATCTGTTGAGTCGCGATGCCATTGACTACGGGc                                                                             >  1:4847836/1‑100 (MQ=255)
                   aTGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGct                                                                            <  1:4853151/100‑1 (MQ=255)
                    tgtgGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctg                                                                           >  2:2167147/1‑100 (MQ=255)
                    tgtgGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctg                                                                           <  1:3863548/100‑1 (MQ=255)
                    tgtgGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctg                                                                           >  2:1007836/1‑100 (MQ=255)
                    tgtgGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctg                                                                           <  1:892184/100‑1 (MQ=255)
                      tgGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctgga                                                                         >  2:4764663/1‑100 (MQ=255)
                      tgGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctgga                                                                         >  2:5385602/1‑100 (MQ=255)
                      tgGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctgga                                                                         >  1:2663503/1‑100 (MQ=255)
                      tgGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCAAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctgga                                                                         >  2:3639217/1‑100 (MQ=255)
                       ggAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctggaa                                                                        >  1:4114032/1‑100 (MQ=255)
                         agaTTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctggaacc                                                                      <  1:1793116/100‑1 (MQ=255)
                         agaTTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctggaacc                                                                      <  1:2932842/100‑1 (MQ=255)
                         agaTTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctggaacc                                                                      >  2:4782752/1‑100 (MQ=255)
                          gaTTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctggaacct                                                                     <  2:3968543/100‑1 (MQ=255)
                           aTTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctggaacctg                                                                    <  2:1987647/100‑1 (MQ=255)
                           aTTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctggaacccg                                                                    >  2:2852483/1‑98 (MQ=255)
                             tGTCGATGGCGGTACTCATGTACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctggaacctgga                                                                  <  1:5219594/100‑1 (MQ=255)
                             tGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctggaacctgga                                                                  >  1:69300/1‑100 (MQ=255)
                              gTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctggaacctggaa                                                                 <  1:3980309/100‑1 (MQ=255)
                                cGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACg                                                               >  1:3585554/1‑100 (MQ=255)
                                cGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACg                                                               >  2:4254517/1‑100 (MQ=255)
                                    ggCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGc                                                           <  1:829003/100‑1 (MQ=255)
                                     gCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCg                                                          >  1:1899771/1‑100 (MQ=255)
                                         tACTCCGGGACTGAACTTGCTGGGGTATGTAGAAAGCGCCAGCCATCTGTTGATTCGCGCTGCCATTGCCTCCGGGCTGGAACCTGGCACGCTGCGAAcc                                                      <  2:4664456/100‑1 (MQ=255)
                                          aCTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCt                                                     >  1:178087/1‑100 (MQ=255)
                                           cTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTa                                                    >  1:652028/1‑100 (MQ=255)
                                           cTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTa                                                    >  1:5451102/1‑100 (MQ=255)
                                           cTCAGGGACTGAACTTGCTGGGGGATGTCGAAAGCGCCAGCCATCGGTTGATTCGCGATGCCATTGACTACGGGCTCGAACCTGGAACGCTGCGAACCTa                                                    <  2:3296404/100‑1 (MQ=255)
                                            tCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTAt                                                   <  2:5459508/100‑1 (MQ=255)
                                             cAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATg                                                  >  2:2727130/1‑100 (MQ=255)
                                             cAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATg                                                  >  2:1258083/1‑100 (MQ=255)
                                               gggACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGcc                                                <  2:3792903/100‑1 (MQ=255)
                                               gggACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGcc                                                <  2:4157609/100‑1 (MQ=255)
                                                 gACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCgg                                              >  2:750482/1‑100 (MQ=255)
                                                 gACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCgg                                              >  1:2857715/1‑100 (MQ=255)
                                                 gACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCgg                                              <  1:4053176/100‑1 (MQ=255)
                                                   cTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGgag                                            >  1:2979174/1‑100 (MQ=255)
                                                   cTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGgag                                            >  1:1085226/1‑100 (MQ=255)
                                                    tGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGgagt                                           >  2:1401938/1‑99 (MQ=255)
                                                     gAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGaa                                          >  1:4673869/1‑100 (MQ=255)
                                                      aaCTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAAc                                         <  1:2965195/100‑1 (MQ=255)
                                                       aCTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACg                                        <  2:4191460/100‑1 (MQ=255)
                                                       aCTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACg                                        <  2:2435419/100‑1 (MQ=255)
                                                        cTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGc                                       >  1:3754126/1‑100 (MQ=255)
                                                            cTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTc                                   >  1:4734532/1‑100 (MQ=255)
                                                             tGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTcc                                  <  1:2182315/100‑1 (MQ=255)
                                                              ggggTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCg                                 <  2:1687433/100‑1 (MQ=255)
                                                              ggggTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCg                                 <  1:4039410/100‑1 (MQ=255)
                                                                ggTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGc                               >  1:243615/1‑100 (MQ=255)
                                                                 gTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCt                              <  2:715600/100‑1 (MQ=255)
                                                                     gTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtc                          >  2:2382854/1‑100 (MQ=255)
                                                                     gTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtc                          >  1:1286851/1‑100 (MQ=255)
                                                                     gTCGAAAGCGCCAGCCATCTATTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtc                          >  1:4350605/1‑100 (MQ=255)
                                                                       cGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtctc                        >  1:3837898/1‑100 (MQ=255)
                                                                       cGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtctc                        >  1:1409699/1‑100 (MQ=255)
                                                                       cGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtctc                        >  2:1164024/1‑100 (MQ=255)
                                                                        gAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCa                       >  2:4772768/1‑100 (MQ=255)
                                                                        gAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCa                       >  2:1283386/1‑100 (MQ=255)
                                                                        gAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCa                       >  2:4432847/1‑100 (MQ=255)
                                                                             cgcCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGcgcg                  >  1:3307291/1‑100 (MQ=255)
                                                                               ccagccaTCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCgaa                >  1:2347777/1‑100 (MQ=255)
                                                                               ccagccaTCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCgaa                <  2:2645820/100‑1 (MQ=255)
                                                                                    caTCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGaaaa           >  1:494721/1‑100 (MQ=255)
                                                                                    caTCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGaaaa           >  1:3120002/1‑100 (MQ=255)
                                                                                     aTCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAAt          <  1:442059/100‑1 (MQ=255)
                                                                                     aTCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAAt          <  2:5086969/100‑1 (MQ=255)
                                                                                     aTCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAAt          <  1:4033276/100‑1 (MQ=255)
                                                                                      tCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATg         <  1:3587264/100‑1 (MQ=255)
                                                                                       cTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGa        >  1:2577480/1‑100 (MQ=255)
                                                                                        tGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAGAATGAg       >  1:5025494/1‑100 (MQ=255)
                                                                                        tGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAg       <  2:3123527/100‑1 (MQ=255)
                                                                                        tGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAg       <  1:4112031/100‑1 (MQ=255)
                                                                                        tGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAg       >  2:1387720/1‑100 (MQ=255)
                                                                                        tGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAg       >  1:3510394/1‑100 (MQ=255)
                                                                                          ttGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGcc     >  1:2606656/1‑100 (MQ=255)
                                                                                          ttGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGcc     <  2:4889804/100‑1 (MQ=255)
                                                                                          ttGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGcc     <  1:2073698/100‑1 (MQ=255)
                                                                                          ttGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGcc     <  2:356762/100‑1 (MQ=255)
                                                                                          ttGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGcc     <  1:1460060/100‑1 (MQ=255)
                                                                                          ttGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGcc     <  1:4272459/100‑1 (MQ=255)
                                                                                          ttGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGcc     <  1:929032/100‑1 (MQ=255)
                                                                                          ttGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGcc     >  1:1233337/1‑100 (MQ=255)
                                                                                          ttGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGcc     <  2:3976207/100‑1 (MQ=255)
                                                                                                 |                                                                                               
CATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGC  >  NC_000913/1035863‑1036055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A