Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,035,960 T→G L64R (CTC→CGC)  hyaD → hydrogenase 1 maturation protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,035,9600TG97.2% 474.0 / 1.6 145L64R (CTC→CGC) hyaDhydrogenase 1 maturation protease
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base G (79/62);  total (82/63)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

Only 100 of 147 total aligned reads displayed.

CATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCA  >  NC_000913/1035863‑1036056
                                                                                                 |                                                                                                
cATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTcgcg                                                                                                <  2:3921962/100‑1 (MQ=255)
cATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTcgcg                                                                                                <  1:52990/100‑1 (MQ=255)
  ttACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGAt                                                                                              <  2:2798569/100‑1 (MQ=255)
   tACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATg                                                                                             <  2:2034641/100‑1 (MQ=255)
    aCCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGc                                                                                            >  1:887208/1‑100 (MQ=255)
    aCCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGc                                                                                            >  2:29429/1‑100 (MQ=255)
    aCCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGc                                                                                            >  1:1897894/1‑100 (MQ=255)
    aCCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGc                                                                                            >  1:885200/1‑100 (MQ=255)
      cACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCa                                                                                          >  1:2972422/1‑100 (MQ=255)
       aCTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCAt                                                                                         >  1:2464443/1‑100 (MQ=255)
         tGGCCCGAGTATGTGGAGATTGTCGACGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTg                                                                                       <  1:1785227/100‑1 (MQ=255)
           gCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGAc                                                                                     <  1:1529966/100‑1 (MQ=255)
           gCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGAc                                                                                     >  1:858648/1‑100 (MQ=255)
            cccGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACt                                                                                    >  1:747967/1‑100 (MQ=255)
              cGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTAc                                                                                  <  2:1027434/100‑1 (MQ=255)
              cGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTAc                                                                                  >  1:2454914/1‑100 (MQ=255)
               gAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACg                                                                                 >  2:2543106/1‑100 (MQ=255)
                  tATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGc                                                                              <  1:2401952/100‑1 (MQ=255)
                  tATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGc                                                                              <  1:190963/100‑1 (MQ=255)
                  tATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGc                                                                              <  2:1973398/100‑1 (MQ=255)
                  tATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGc                                                                              <  1:2285480/100‑1 (MQ=255)
                  tATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCGGTTGGTTCGCGATGCCATTGACTACGGGc                                                                              <  2:1925930/100‑1 (MQ=255)
                   ctgtgGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGct                                                                             <  1:1444086/99‑1 (MQ=255)
                   aTGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGct                                                                             <  1:257754/100‑1 (MQ=255)
                   aTGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGct                                                                             <  1:2156821/100‑1 (MQ=255)
                    tgtgGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctg                                                                            >  1:2861720/1‑100 (MQ=255)
                    tgtgGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctg                                                                            >  1:3829754/1‑100 (MQ=255)
                      tgGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctgga                                                                          >  1:4032223/1‑100 (MQ=255)
                      tgGAGATTGTCGATGGCGGTACTAAGGGAATGAACTTGCGGGGATATGTCGAAAGCGCCAGCCATATGTTGATTAGCTCTGGCATTAACTACGGGctgga                                                                          >  2:2935909/1‑100 (MQ=255)
                         agaTTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctggaacc                                                                       <  1:1041223/100‑1 (MQ=255)
                         agaTTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctggaacc                                                                       >  1:2620377/1‑100 (MQ=255)
                          gaTTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGAATGTCGAAAGCGCCAGCCATCTGTTGATTCGGGATGCCATTGACTACGGGctggaacct                                                                      >  1:3539136/1‑100 (MQ=255)
                           aTTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctggaacctg                                                                     >  1:164775/1‑100 (MQ=255)
                            ttGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctggaacctgg                                                                    >  2:2756007/1‑100 (MQ=255)
                            ttGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGGCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGTctggaacctgg                                                                    >  2:3802504/1‑100 (MQ=255)
                             tGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctggaacctgga                                                                   <  2:296030/100‑1 (MQ=255)
                              gTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctggaacctggaa                                                                  >  1:300990/1‑100 (MQ=255)
                               tCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctggaacctggaac                                                                 <  2:3137121/100‑1 (MQ=255)
                               tCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGCATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGctggaacctggaac                                                                 <  1:2744407/100‑1 (MQ=255)
                                 gATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGc                                                               >  2:2994186/1‑100 (MQ=255)
                                 gATGGCGGTACTCAGGGACTGAACTTGCT‑GGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCt                                                              >  2:2447148/1‑100 (MQ=255)
                                   tGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTg                                                             <  1:2728601/100‑1 (MQ=255)
                                     gCGGTACTCAGGGACTGAACTTGCTGGGGTATGGCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCg                                                           <  1:580197/100‑1 (MQ=255)
                                      cGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGa                                                          >  2:3546912/1‑100 (MQ=255)
                                        gTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAAc                                                        >  1:2045092/1‑100 (MQ=255)
                                         tACTCTGGGGCAGAAGTTGCAGGGGTATGCGGAAGGCGTAAGCCATCGGTTGATGCGCGATGTAATTGACTACGGGCTGGAACCTGGAACGCTGCGAAcc                                                       <  2:1962100/100‑1 (MQ=255)
                                         tACTCAGGGACTGAACTTGCTGGGGTTTGTCTAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTTGACCCTGTAACGCTGTGAAcc                                                       <  2:445130/100‑1 (MQ=255)
                                         tACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAAcc                                                       <  2:2340593/100‑1 (MQ=255)
                                          aCTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCt                                                      >  1:3932303/1‑100 (MQ=255)
                                                   cTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGgag                                             <  1:3685285/100‑1 (MQ=255)
                                                   cTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGgag                                             <  2:33340/100‑1 (MQ=255)
                                                    tGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGgaga                                            <  2:938163/100‑1 (MQ=255)
                                                     gAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGaa                                           >  1:51299/1‑100 (MQ=255)
                                                     gAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGaa                                           >  1:3679807/1‑100 (MQ=255)
                                                     gAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGaa                                           >  2:591099/1‑100 (MQ=255)
                                                        gttGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCCGGAACGCTGCGAACCTATGCCGGAGAACGc                                        <  2:1985141/99‑1 (MQ=255)
                                                        cTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGc                                        >  2:3668938/1‑100 (MQ=255)
                                                        cTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGc                                        <  2:1985286/100‑1 (MQ=255)
                                                          tGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCAt                                      <  1:1568864/100‑1 (MQ=255)
                                                              ggggTATGTCGAAAGCGCCAGCCATTTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCg                                  >  1:2830419/1‑100 (MQ=255)
                                                              ggggTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCg                                  >  2:505904/1‑100 (MQ=255)
                                                              ggggTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCg                                  <  2:1757666/100‑1 (MQ=255)
                                                              ggggTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCg                                  >  2:278740/1‑100 (MQ=255)
                                                                ggTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGc                                <  1:2085330/100‑1 (MQ=255)
                                                                ggTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGc                                >  1:1577615/1‑100 (MQ=255)
                                                                 gTATGTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCt                               <  2:3315655/100‑1 (MQ=255)
                                                                     gTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACTCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtc                           >  2:1312931/1‑100 (MQ=255)
                                                                     gTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtc                           >  1:2166779/1‑100 (MQ=255)
                                                                     gTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtc                           <  2:1867545/100‑1 (MQ=255)
                                                                     gTCGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtc                           >  1:1015436/1‑100 (MQ=255)
                                                                     gTCGAAAGCGCAAGACGTCTGTTGATTCGCGATGCCATTGACTAGGGGATGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtc                           >  1:3578809/1‑100 (MQ=255)
                                                                       cGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtctc                         <  1:100949/100‑1 (MQ=255)
                                                                       cGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtctc                         >  2:2840607/1‑100 (MQ=255)
                                                                       cGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtctc                         <  2:2417153/100‑1 (MQ=255)
                                                                       cGAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtctc                         >  2:1698694/1‑100 (MQ=255)
                                                                        gAAAGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCa                        <  1:2334047/100‑1 (MQ=255)
                                                                         aaaGCTCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAg                       <  2:1652530/100‑1 (MQ=255)
                                                                         aaaGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAg                       <  2:2324262/100‑1 (MQ=255)
                                                                         aaaGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAg                       <  2:2255393/100‑1 (MQ=255)
                                                                          aaGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTATGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAgc                      >  2:926275/1‑100 (MQ=255)
                                                                          aaGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAgn                      <  1:205460/100‑2 (MQ=255)
                                                                          aaGCGCCAGCCATCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAgc                      >  1:196778/1‑100 (MQ=255)
                                                                               ccagccaTCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCgaa                 >  1:3099020/1‑100 (MQ=255)
                                                                               ccagccaTCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCgaa                 >  2:751647/1‑100 (MQ=255)
                                                                                cagccaTCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCgaag                <  1:1719387/100‑1 (MQ=255)
                                                                                cagccaTCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCgaag                >  1:3343426/1‑100 (MQ=255)
                                                                                 agccaTCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCgaaga               >  2:1047153/1‑100 (MQ=255)
                                                                                 agccaTCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCgaaga               >  2:74462/1‑100 (MQ=255)
                                                                                  gccaTCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCgaagaa              >  2:2230387/1‑100 (MQ=255)
                                                                                    caTCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGaaaa            >  1:2527910/1‑100 (MQ=255)
                                                                                     aTCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAAt           <  1:1222239/100‑1 (MQ=255)
                                                                                     aTCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAAt           >  2:250434/1‑100 (MQ=255)
                                                                                      tCTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATg          <  2:544147/100‑1 (MQ=255)
                                                                                       cTGTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGa         >  2:3548282/1‑100 (MQ=255)
                                                                                         gTTGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGc       >  2:1459199/1‑100 (MQ=255)
                                                                                          ttGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGcc      <  1:3883828/100‑1 (MQ=255)
                                                                                          ttGATTCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGcc      <  2:1248931/100‑1 (MQ=255)
                                                                                          ttGATTCGCGATGCCATTGACGACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCAGGCTTATCTCAGCGCGAAGAAAATGAGcc      <  2:593486/100‑1 (MQ=255)
                                                                                          ttGATCCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGcc      <  1:1140462/100‑1 (MQ=255)
                                                                                              ttCGCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCa  >  1:333672/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCA  >  NC_000913/1035863‑1036056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A