Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I52 R1	239	220.4	11079197	93.2%	10325811	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,224,314	T→G	N78H (AAC→CAC)	minE ←	cell division topological specificity factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,224,314	0	T	G	99.5%	685.6 / ‑4.2	195	N78H (AAC→CAC)	minE	cell division topological specificity factor
Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (96/98); total (97/98)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01

Only 100 of 195 total aligned reads displayed.

GGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAA  >  NC_000913/1224217‑1224409

ggATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCa                                                                                               <  1:5295424/100‑1 (MQ=255)

   tGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCTTTCTTTTTTCAGCCCTTCTGCTTCCGGTAAGGTCACGTGCAGCt                                                                                            >  2:2189/1‑100 (MQ=255)

     aaaaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCa                                                                                          <  1:360657/100‑1 (MQ=255)

     aaaaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCa                                                                                          >  1:2666202/1‑100 (MQ=255)

       aaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAg                                                                                        <  1:1107979/100‑1 (MQ=255)

        aaCTCAAGGCAGAGATAACTCTGCCTTGCAGATAAATGCGATTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGa                                                                                       <  2:1109824/100‑1 (MQ=255)

        aaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGa                                                                                       <  1:795122/100‑1 (MQ=255)

         cttCAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGaa                                                                                      >  2:4637943/3‑100 (MQ=38)

         aCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGaa                                                                                      <  1:736554/100‑1 (MQ=255)

          cTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAAt                                                                                     >  2:16221/1‑100 (MQ=255)

           tCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATa                                                                                    >  1:1095995/1‑100 (MQ=255)

            cAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAg                                                                                   >  2:4527329/1‑100 (MQ=255)

             aaGGCAGAGTTATCTCTGCCTTGAAGATGAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGa                                                                                  >  1:4894351/1‑100 (MQ=255)

             aaGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGa                                                                                  >  1:3159789/1‑100 (MQ=255)

             aaGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGa                                                                                  >  1:1581197/1‑100 (MQ=255)

               ggCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTATAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGaaa                                                                                >  1:2306977/1‑100 (MQ=255)

               ggCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGaaa                                                                                >  1:862843/1‑100 (MQ=255)

               ggCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGaaa                                                                                >  2:4249783/1‑100 (MQ=255)

               ggCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGaaa                                                                                >  1:3058585/1‑100 (MQ=255)

                   gagaTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGGATAGAAATATc                                                                            <  2:3280554/100‑1 (MQ=255)

                     gaTAGCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGc                                                                          <  1:2567984/100‑1 (MQ=255)

                     gaTAACTCTGCCTTGAAGATAAATGCTCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGc                                                                          <  1:2070831/100‑1 (MQ=255)

                      aTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTAAAGAATAGAAATATCGcc                                                                         >  2:3303028/1‑100 (MQ=255)

                         aCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATc                                                                      <  1:2479772/100‑1 (MQ=255)

                             tGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCtttt                                                                  <  1:602219/100‑1 (MQ=255)

                              gCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCttttt                                                                 <  2:2333068/100‑1 (MQ=255)

                                cTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCGGGTGAGGTCACGTGCAGCTCTAGAATAGAAATATCGCCATCtttttcc                                                               >  2:3739925/1‑98 (MQ=255)

                                cTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGc                                                               >  1:2648756/1‑100 (MQ=255)

                                 ttGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCt                                                              >  1:95119/1‑100 (MQ=255)

                                  tGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTc                                                             >  2:2215900/1‑100 (MQ=255)

                                   gAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCa                                                            >  2:4067149/1‑100 (MQ=255)

                                     aGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAg                                                          <  2:4864902/100‑1 (MQ=255)

                                      gATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGc                                                         >  1:5382545/1‑100 (MQ=255)

                                      gATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGc                                                         >  1:4016248/1‑100 (MQ=255)

                                      gATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGc                                                         <  1:4683242/100‑1 (MQ=255)

                                       aTAAATGCGCTTTTACTTTTTTCTTATTTCAGCTATTCTGCCTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCCCCATCAGTTTTCTCAAGCt                                                        >  2:1908494/1‑100 (MQ=255)

                                          aaTGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTa                                                     >  2:138418/1‑100 (MQ=255)

                                          aaTGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTa                                                     <  2:143727/100‑1 (MQ=255)

                                          aaTGCGATTTTACAGCGGACTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTAAAGCTGTa                                                     >  1:4648297/1‑100 (MQ=255)

                                           aTGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTAc                                                    <  1:678599/100‑1 (MQ=255)

                                           aTGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTAc                                                    <  2:1015723/100‑1 (MQ=255)

                                           aTGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTAc                                                    <  2:2024134/100‑1 (MQ=255)

                                            tGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACg                                                   <  2:4418765/100‑1 (MQ=255)

                                             gcgcTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACgg                                                  <  2:1561782/100‑1 (MQ=255)

                                              cgcTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGt                                                 >  2:2257341/1‑100 (MQ=255)

                                                 ttttACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTAc                                              <  2:668692/100‑1 (MQ=255)

                                                 ttttACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTAc                                              <  1:147996/100‑1 (MQ=255)

                                                 ttttACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTAc                                              >  1:1843309/1‑100 (MQ=255)

                                                  tttACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTAcc                                             <  2:860976/100‑1 (MQ=255)

                                                   ttACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAATGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCa                                            <  2:5303626/100‑1 (MQ=255)

                                                   ttACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCa                                            <  2:2637150/100‑1 (MQ=255)

                                                   ttACAGCGGGCTTATTCCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCa                                            <  2:620862/100‑1 (MQ=255)

                                                     aCAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCAtc                                          >  2:714088/1‑100 (MQ=255)

                                                       aGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCAtctc                                        <  1:4250270/100‑1 (MQ=255)

                                                        gCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCa                                       >  1:2165481/1‑100 (MQ=255)

                                                         cgcgCTTATTTCATCTCTTCTGCTTCCGGTAAGGTCACGGGCAGCTCAAGAATAAAAATATCCCCATCTTTTTGCTCAAGCGGTACGGTTACCATCTCat                                      <  2:5016389/97‑2 (MQ=255)

                                                         cGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAg                                      <  1:5408894/100‑1 (MQ=255)

                                                         cGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAg                                      <  2:4007613/100‑1 (MQ=255)

                                                         cGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAg                                      >  1:4477600/1‑100 (MQ=255)

                                                          gggCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAgg                                     <  1:2837690/100‑1 (MQ=255)

                                                           ggCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGa                                    >  1:4093353/1‑100 (MQ=255)

                                                            gCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGAt                                   <  1:3053102/100‑1 (MQ=255)

                                                            gCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGAt                                   <  2:3396701/100‑1 (MQ=255)

                                                             cTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATc                                  >  1:4855389/1‑100 (MQ=255)

                                                             cTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATc                                  >  1:2606447/1‑100 (MQ=255)

                                                             cTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATc                                  >  2:1851442/1‑100 (MQ=255)

                                                              ttATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCa                                 <  1:3918455/100‑1 (MQ=255)

                                                              ttATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCa                                 <  1:4289110/100‑1 (MQ=255)

                                                                 tttCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAAtt                              <  1:3000165/100‑1 (MQ=255)

                                                                 tttCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAAtt                              <  2:2885279/100‑1 (MQ=255)

                                                                  ttCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAAttt                             >  1:5091414/1‑100 (MQ=255)

                                                                   tCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTg                            >  2:5441612/1‑100 (MQ=255)

                                                                   tCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTg                            <  2:3121265/100‑1 (MQ=255)

                                                                    cAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGt                           >  2:3657547/1‑100 (MQ=255)

                                                                    cAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGt                           >  1:3015733/1‑100 (MQ=255)

                                                                     aGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTa                          <  2:3508067/100‑1 (MQ=255)

                                                                     aGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTa                          <  1:1978211/100‑1 (MQ=255)

                                                                     aGCTCTTCTGCGTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTa                          <  1:4093080/100‑1 (MQ=255)

                                                                       ctctTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACg                        <  2:4829936/100‑1 (MQ=255)

                                                                        tcttctGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTTCTCAAGCTGTACGGTTACCATCTCAGGATCACTTTGTACGt                       >  2:5084515/1‑100 (MQ=255)

                                                                         cttctGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTa                      >  2:244889/1‑100 (MQ=255)

                                                                           tctGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTAtt                    <  2:4578811/100‑1 (MQ=255)

                                                                           tctGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTAtt                    >  1:4525517/1‑100 (MQ=255)

                                                                            ctGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTAttt                   >  1:1406117/1‑100 (MQ=255)

                                                                              gCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTTCTTAATTTTTACGGTTACAATCTCAGGATCAATTTGTACGTATTTAc                 >  2:2978758/1‑100 (MQ=255)

                                                                               cTTCCGGTAAGGTCACGTGCAGCTCAAGCATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACa                <  2:4822718/100‑1 (MQ=255)

                                                                                  ccGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAAt             <  2:917316/100‑1 (MQ=255)

                                                                                  ccGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAAt             <  2:3673376/100‑1 (MQ=255)

                                                                                   cGGTTAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCATGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATg            <  1:4987251/100‑1 (MQ=255)

                                                                                   cGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATg            >  1:3968885/1‑100 (MQ=255)

                                                                                   cGGTAAGGTCACGTGCAGCTCAAGAATAAAAATATCGCCATCTTTTTGCTCAAGCTGTAAGGATACCATCTCAGGATCAATTTGTACGTATTTACAAATg            >  1:2572300/1‑100 (MQ=255)

                                                                                    ggTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCGAGCTGTACGGTTACCATCTCAGGATCAATTTGTACATATTTACAAATGa           >  1:5022758/1‑100 (MQ=255)

                                                                                    ggTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGa           >  1:5004727/1‑100 (MQ=255)

                                                                                      tAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGAcc         <  1:5123853/100‑1 (MQ=255)

                                                                                       aaGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCt        <  1:3450479/100‑1 (MQ=255)

                                                                                         ggTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCa      <  1:4576396/100‑1 (MQ=255)

                                                                                         ggTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCa      >  2:1299911/1‑100 (MQ=255)

                                                                                           tCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAg    <  2:4950568/100‑1 (MQ=255)

                                                                                            cACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGa   >  1:1239089/1‑100 (MQ=255)

                                                                                            cACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGa   <  1:1675104/100‑1 (MQ=255)

GGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAA  >  NC_000913/1224217‑1224409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A