Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,224,314 T→G N78H (AAC→CAC)  minE ← cell division topological specificity factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,224,3140TG98.1% 526.5 / 0.1 156N78H (AAC→CAC) minEcell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base G (94/59);  total (95/61)
Fisher's exact test for biased strand distribution p-value = 5.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

Only 100 of 156 total aligned reads displayed.

GGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAA  >  NC_000913/1224217‑1224409
                                                                                                 |                                                                                               
 gATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAg                                                                                              >  1:537742/1‑100 (MQ=255)
 gATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAg                                                                                              <  2:2170003/100‑1 (MQ=255)
 gATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCCCTTCTGCTTCCGGTAAGGTCACGTGCAg                                                                                              >  2:3423485/1‑100 (MQ=255)
  aTGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGc                                                                                             >  2:781523/1‑100 (MQ=255)
  aTGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGc                                                                                             >  2:2727088/1‑100 (MQ=255)
  aTGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGc                                                                                             >  2:2890613/1‑100 (MQ=255)
   tGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCt                                                                                            <  1:3631029/100‑1 (MQ=255)
   tGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCt                                                                                            >  2:3140901/1‑100 (MQ=255)
    gAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTCCCGGTAAGGTCACGTGCAGccc                                                                                           >  2:912190/1‑98 (MQ=255)
       aaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATACATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTAACGTGCAGCTCAAg                                                                                        <  2:1758284/100‑1 (MQ=255)
       aaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAg                                                                                        <  1:2934568/100‑1 (MQ=255)
            cAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAg                                                                                   <  1:3205259/100‑1 (MQ=255)
            cAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAg                                                                                   <  1:1996170/100‑1 (MQ=255)
            cAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAg                                                                                   <  1:2795954/100‑1 (MQ=255)
             aaGGCAGAGTTATCACTGCCTTGAAGATAAATGCGCTTTTACAGCGGACTTATTCCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGa                                                                                  >  2:3740224/1‑100 (MQ=255)
             aaGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGa                                                                                  <  2:166491/100‑1 (MQ=255)
              aGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGaa                                                                                 >  1:516947/1‑100 (MQ=255)
               ggCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGaaa                                                                                >  1:1491470/1‑100 (MQ=255)
               ggCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGaaa                                                                                >  2:2471662/1‑100 (MQ=255)
                 cAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAata                                                                              <  2:1589323/100‑1 (MQ=255)
                  agagATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAatat                                                                             >  1:3402985/1‑100 (MQ=255)
                     gttatctctGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGc                                                                          >  1:1701449/6‑100 (MQ=255)
                     gaTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGc                                                                          <  2:1755808/100‑1 (MQ=255)
                       tAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCa                                                                        >  2:2083307/1‑100 (MQ=255)
                          ctctGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCt                                                                     >  2:2225733/1‑100 (MQ=255)
                          ctctGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCAtat                                                                     <  2:751580/100‑3 (MQ=255)
                           tctGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCtt                                                                    >  1:1394626/1‑100 (MQ=255)
                            ctGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCttt                                                                   >  1:881068/1‑100 (MQ=255)
                             tGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCtttt                                                                  <  1:3182744/100‑1 (MQ=255)
                              gCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCttttt                                                                 >  1:2045814/1‑100 (MQ=255)
                              gCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCttttt                                                                 <  2:2728911/100‑1 (MQ=255)
                               ccTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTg                                                                <  1:2471942/100‑1 (MQ=255)
                                cTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGc                                                               >  2:710274/1‑100 (MQ=255)
                                  tGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTc                                                             >  1:1789084/1‑100 (MQ=255)
                                    aaGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCaa                                                           >  1:272894/1‑100 (MQ=255)
                                     aGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAg                                                          >  2:1892075/1‑100 (MQ=255)
                                       aTAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTctttct                                                        >  2:1817463/1‑95 (MQ=255)
                                        tAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTg                                                       <  1:44607/100‑1 (MQ=255)
                                         aaaTGAGCTTTTACAGCGGGCTTATTTCAGCTCTTAGGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGt                                                      >  2:1330372/1‑100 (MQ=255)
                                           aTGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTAc                                                    <  1:658139/100‑1 (MQ=255)
                                            tGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACg                                                   >  1:2668578/1‑100 (MQ=255)
                                              cgcTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGt                                                 >  1:1132948/1‑100 (MQ=255)
                                              cgcTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGt                                                 >  2:2687250/1‑100 (MQ=255)
                                              cgcTATTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCAGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGt                                                 >  2:1688058/1‑100 (MQ=255)
                                                      cAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATGGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCAtct                                         >  1:2966342/1‑100 (MQ=255)
                                                      cAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCAtct                                         >  1:1416191/1‑100 (MQ=255)
                                                       aGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCAtctc                                        <  2:1992865/100‑1 (MQ=255)
                                                        gCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCa                                       <  1:2518055/100‑1 (MQ=255)
                                                        gCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCa                                       >  1:1045993/1‑100 (MQ=255)
                                                         cGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAg                                      <  1:3053765/100‑1 (MQ=255)
                                                          gggCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAgg                                     <  1:1757197/100‑1 (MQ=255)
                                                          gggCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAgg                                     >  2:1046266/1‑100 (MQ=255)
                                                          gggCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAgg                                     <  2:2201474/100‑1 (MQ=255)
                                                           ggCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGa                                    <  2:1351680/100‑1 (MQ=255)
                                                            gCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGAt                                   >  1:1940848/1‑100 (MQ=255)
                                                            gCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGAt                                   <  1:3083618/100‑1 (MQ=255)
                                                            gCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGAt                                   >  2:711791/1‑100 (MQ=255)
                                                               tATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCaa                                <  2:3234383/100‑1 (MQ=255)
                                                               tATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCaa                                <  1:3762897/100‑1 (MQ=255)
                                                                aTTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAAt                               <  1:2735851/100‑1 (MQ=255)
                                                                 tttCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAAtt                              <  2:1399611/100‑1 (MQ=255)
                                                                  ttCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAAttt                             >  1:3548573/1‑100 (MQ=255)
                                                                  ntCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAAttt                             >  2:3655825/2‑100 (MQ=255)
                                                                   tCAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTg                            <  1:3927139/100‑1 (MQ=255)
                                                                    cAGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGt                           <  1:1080156/100‑1 (MQ=255)
                                                                     aGCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTa                          <  1:400911/100‑1 (MQ=255)
                                                                      gCTCTTCTGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTAc                         <  1:3607473/100‑1 (MQ=255)
                                                                        tcttctGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGt                       >  1:2787882/1‑100 (MQ=255)
                                                                        tcttctGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGt                       <  1:2426613/100‑1 (MQ=255)
                                                                        tcttctGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGt                       >  1:3714883/1‑100 (MQ=255)
                                                                        tattctGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCGCAAGCTGTACGGCTACCCTCTCAGGATCCATTTGTACGt                       >  2:489339/3‑100 (MQ=255)
                                                                         cttctGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTa                      >  1:1809567/1‑100 (MQ=255)
                                                                           tctGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTAtt                    >  1:1188619/1‑100 (MQ=255)
                                                                           tctGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTAtt                    >  1:1188598/1‑100 (MQ=255)
                                                                           tctGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTAtt                    <  2:2478279/100‑1 (MQ=255)
                                                                           tctGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTAtt                    <  1:2607747/100‑1 (MQ=255)
                                                                           tctGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTAtt                    >  1:3062625/1‑100 (MQ=255)
                                                                            ctGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTAttt                   >  1:774687/1‑100 (MQ=255)
                                                                             tGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTa                  >  1:1107900/1‑100 (MQ=255)
                                                                             tGCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTa                  <  1:1629386/100‑1 (MQ=255)
                                                                              gCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTAc                 >  1:2023350/1‑100 (MQ=255)
                                                                              gCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTAc                 >  2:1203403/1‑100 (MQ=255)
                                                                              gCTTCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTAc                 >  1:2426567/1‑100 (MQ=255)
                                                                                 tCCGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACaaa              >  1:640348/1‑100 (MQ=255)
                                                                                  ccGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAAt             >  2:2629791/1‑100 (MQ=255)
                                                                                   cGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATg            >  1:2658785/1‑100 (MQ=255)
                                                                                   cGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATg            >  1:449717/1‑100 (MQ=255)
                                                                                   cGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATg            >  2:3533058/1‑100 (MQ=255)
                                                                                   cGGTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTACTCAAGCTGTACGGTTACCATCTCATGATCAATTTGTACGTATTTACAAATg            <  1:3209491/100‑1 (MQ=255)
                                                                                    ggTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTTTTTTTTTTTTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACACATGa           >  2:1742776/1‑100 (MQ=255)
                                                                                    ggTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGa           >  2:1122866/1‑100 (MQ=255)
                                                                                    ggTAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGa           >  1:3504223/1‑100 (MQ=255)
                                                                                      tAAGGTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGAcc         <  2:506363/100‑1 (MQ=255)
                                                                                      tAACGTCAGGTGCCGCTCAAGAATAGAAATATACCCATCTCTTTTCGCTCTCTGTACGCGCAGCATCACAGAAACAATTTGTACGTATTTTCAAATGAcc         >  2:3459793/1‑100 (MQ=255)
                                                                                       aaGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCt        <  1:2260368/100‑1 (MQ=255)
                                                                                         ggTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCa      >  1:4003155/1‑100 (MQ=255)
                                                                                         ggTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATAACCtaa      >  2:3295958/1‑98 (MQ=255)
                                                                                          gTCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCaa     >  2:3274765/1‑100 (MQ=255)
                                                                                           tCACGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAg    <  1:2297950/100‑1 (MQ=255)
                                                                                             aCGTGCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGaa  <  1:2984303/100‑1 (MQ=255)
                                                                                                 |                                                                                               
GGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAA  >  NC_000913/1224217‑1224409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A