Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,462,889 (C)9→8 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,462,8810C.95.0% 878.9 / 42.8 203intergenic (+235/‑131)fadL/yfdFlong‑chain fatty acid outer membrane transporter/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (8/2);  new base . (87/103);  total (96/107)
Fisher's exact test for biased strand distribution p-value = 4.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

TAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATAT  >  NC_000913/2462782‑2462975
                                                                                                   |                                                                                              
tAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTc                                                                                                >  1:4280842/1‑100 (MQ=255)
tAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCGCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTc                                                                                                >  2:878122/1‑100 (MQ=255)
  aGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTccc                                                                                              >  1:2092776/1‑100 (MQ=255)
       tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccc                                                                                         >  2:3457037/1‑100 (MQ=255)
       tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccc                                                                                         <  1:4252543/100‑1 (MQ=255)
       tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACCTTTCTGCATTTCCAGGATACTcccccccc                                                                                         <  2:260633/100‑1 (MQ=255)
        aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccct                                                                                        >  1:560654/1‑99 (MQ=255)
         tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctg                                                                                       <  1:1594036/100‑3 (MQ=255)
         tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctg                                                                                       <  2:1267981/100‑3 (MQ=255)
           cacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctggc                                                                                     <  1:3091952/100‑5 (MQ=255)
           cacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctggc                                                                                     >  1:536255/1‑96 (MQ=255)
           cacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctggc                                                                                     >  2:2859732/1‑96 (MQ=255)
           cacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctggc                                                                                     >  2:5236651/1‑96 (MQ=255)
           cacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctggc                                                                                     <  1:1395599/100‑5 (MQ=255)
           cacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctggc                                                                                     >  1:3724930/1‑96 (MQ=255)
           cacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGCCCTTCTCAAAGACTTTACTTTTCTGCATTTTCAGGATACTcccccccctctc                                                                                     >  2:1144179/1‑96 (MQ=255)
              aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTAt                                                                                 >  1:1821869/1‑100 (MQ=255)
               gACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTAtt                                                                                >  1:3212295/1‑100 (MQ=255)
                aCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtg                                                                               <  1:2386992/100‑1 (MQ=255)
                 cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtgt                                                                              >  2:2455702/1‑100 (MQ=255)
                 cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtgt                                                                              >  1:5242246/1‑100 (MQ=255)
                 cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑GCCCCCCCCTGGCTATtgt                                                                              <  1:1039930/100‑1 (MQ=255)
                  aaaCCTGGAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtgtg                                                                             <  2:2286786/100‑1 (MQ=255)
                  aaaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtgtg                                                                             <  2:2905191/100‑1 (MQ=255)
                   aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTgc                                                                            >  1:116488/1‑100 (MQ=255)
                      cTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCt                                                                         >  2:3253942/1‑100 (MQ=255)
                       tGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTc                                                                        >  1:2860895/1‑100 (MQ=255)
                       tGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTc                                                                        <  1:2278664/100‑1 (MQ=255)
                         aaGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCAt                                                                      <  1:3489411/100‑1 (MQ=255)
                          aGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATa                                                                     <  2:1662140/100‑1 (MQ=255)
                           gggAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATac                                                                    >  2:817893/1‑100 (MQ=255)
                           gggAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATac                                                                    <  2:4467968/100‑1 (MQ=255)
                           gggAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGCTTCCTTTTCTGCATTTCCAGAATCCT‑ACCCCCCCTGCCGATTGTTCGCTCAtcc                                                                    >  1:4761161/1‑98 (MQ=255)
                            ggAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATaca                                                                   >  1:460235/1‑100 (MQ=255)
                              aaaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACt                                                                 >  1:225057/1‑100 (MQ=255)
                              aaaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACt                                                                 <  2:1829015/100‑1 (MQ=255)
                                 ggCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCaa                                                              >  1:3564470/1‑100 (MQ=255)
                                 ggCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCaa                                                              >  2:4035154/1‑100 (MQ=255)
                                   cATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAAt                                                            <  2:2154789/100‑1 (MQ=255)
                                    atatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAAt                                                            >  2:1732027/1‑100 (MQ=255)
                                    atatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAAtt                                                           >  1:3866803/1‑100 (MQ=255)
                                    atatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAAtt                                                           <  2:2833716/100‑1 (MQ=255)
                                    atatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAAtt                                                           <  1:482686/100‑1 (MQ=255)
                                    atatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGATTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAAtt                                                           >  2:738571/1‑100 (MQ=255)
                                     tatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTa                                                          >  2:70795/1‑100 (MQ=255)
                                       ttttCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTaaa                                                        >  1:3593664/1‑100 (MQ=255)
                                       ttttCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTaaa                                                        <  1:3669984/100‑1 (MQ=255)
                                        tttCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAg                                                       <  1:828678/100‑1 (MQ=255)
                                           cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATa                                                    >  2:515861/1‑100 (MQ=255)
                                           cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATa                                                    >  2:4128299/1‑100 (MQ=255)
                                           cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATa                                                    >  1:3391401/1‑100 (MQ=255)
                                           cAGGCGTTCTGCTCGTCCATCTCAAAGAGTTTAATTTTCTGCATTTCCAGGATAC‑AAAACAACCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATa                                                    <  2:4288115/100‑1 (MQ=255)
                                             ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAgg                                                  >  1:3816957/1‑100 (MQ=255)
                                               cGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                <  2:3747657/100‑1 (MQ=255)
                                                gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTc                                               >  2:1968658/1‑100 (MQ=255)
                                                     gCTTGTCCTTCACAAACAGTTAACTTTTCTAAATTAGCAGGATACT‑CACCCACCTGGCTATTATCCTCCAATACACTCAAATTAAATATAGGTTCtaaat                                          <  2:210780/100‑1 (MQ=255)
                                                     gCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaat                                          >  2:385734/1‑100 (MQ=255)
                                                      cTCGTCCTTCTCAAAGAGTTTACTTTTTTTTATTTCCAGGATACTCCCCCCCCC‑GGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaata                                         <  2:1230424/100‑1 (MQ=255)
                                                      cTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaata                                         >  2:4783538/1‑100 (MQ=255)
                                                      cTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaata                                         <  1:5245508/100‑1 (MQ=255)
                                                        cGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaataaa                                       <  2:854449/100‑1 (MQ=255)
                                                           ccTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGa                                    >  1:362134/1‑100 (MQ=255)
                                                              tctcAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCg                                 >  2:4999865/1‑100 (MQ=255)
                                                              tctcAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCg                                 >  2:5274190/1‑100 (MQ=255)
                                                                tcAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGtt                               <  2:3182627/100‑1 (MQ=255)
                                                                 cAAAGAGTTTACTTGTCGGCATGTCCAGGATAC‑GCCCCCCCCGGGCTATTGGGCGCTCATACACTCAAATTAAAGAGAGGTTCTAAATAATGGATCGttt                              >  2:3025571/1‑100 (MQ=255)
                                                                  aaaGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGtttt                             >  1:2978439/1‑100 (MQ=255)
                                                                   aaGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGttttt                            <  2:3338207/100‑1 (MQ=255)
                                                                    agagTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGtttttt                           <  1:5427045/100‑1 (MQ=255)
                                                                       gTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGAt                        <  2:427735/100‑1 (MQ=255)
                                                                       gTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGAt                        <  1:2709156/100‑1 (MQ=255)
                                                                        tttACTTTTCTGCATTTCCAGGATCCT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa                       <  1:4166560/100‑1 (MQ=255)
                                                                         ttACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAg                      <  1:3437221/100‑1 (MQ=255)
                                                                         ttACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAg                      >  2:297423/1‑100 (MQ=255)
                                                                         ttACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAg                      >  1:1712953/1‑100 (MQ=255)
                                                                          tACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAg                      >  1:467654/1‑100 (MQ=255)
                                                                          tACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAg                      >  1:3183828/1‑100 (MQ=255)
                                                                          tACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAGATAAATGAGCGTTTTTTGATAGt                     >  2:1851234/1‑100 (MQ=255)
                                                                          tACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGt                     >  1:2958228/1‑100 (MQ=255)
                                                                           aCTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATaaga                    >  2:511289/1‑97 (MQ=255)
                                                                           aCTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTc                    <  1:2031678/100‑1 (MQ=255)
                                                                           aCTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTc                    <  2:624439/100‑1 (MQ=255)
                                                                            cTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCt                   >  2:4932599/1‑100 (MQ=255)
                                                                            cTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCt                   <  1:1282686/100‑1 (MQ=255)
                                                                             ttttCTGCATTTCCAGGATACT‑‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTAt                 >  2:5436079/1‑100 (MQ=255)
                                                                                tCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTAttt               >  2:378748/1‑100 (MQ=255)
                                                                                tCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTAttt               <  2:2387153/100‑1 (MQ=255)
                                                                                tCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTAttt               >  1:906725/1‑100 (MQ=255)
                                                                                tCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTAttt               >  1:3890991/1‑100 (MQ=255)
                                                                                 cTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTc              >  1:1943695/1‑100 (MQ=255)
                                                                                 cTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTc              >  1:962591/1‑100 (MQ=255)
                                                                                  tGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa             >  1:2529301/1‑100 (MQ=255)
                                                                                  tGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa             <  2:1073329/100‑1 (MQ=255)
                                                                                   gCATTTCCAGGATACT‑CCCCCCCCTTTCTAGTGTGCTTTCATACACGCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt            <  2:3918558/100‑1 (MQ=255)
                                                                                   gCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt            >  1:4978224/1‑100 (MQ=255)
                                                                                    cATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAGATAAATGAGCGTTTTTTGATAGTCTATTTCAtt           <  1:1872078/100‑1 (MQ=255)
                                                                                      tttCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAg         <  2:3531517/100‑1 (MQ=255)
                                                                                      tttCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAAAAAATGAGCGTTTTTTGATAGTCTATTTCATTAg         >  1:5509363/1‑100 (MQ=255)
                                                                                          cAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTTAAATTAAAGATAGGTTTTAAAAATATGAGCGTTTTTTGATAGTCTATTTCATTAGGTaa     <  2:5366724/100‑1 (MQ=255)
                                                                                            ggATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAata   >  1:5327763/1‑100 (MQ=255)
                                                                                                   |                                                                                              
TAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATAT  >  NC_000913/2462782‑2462975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A