Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 783,757 A→C I198L (ATT→CTT)  pnuC → nicotinamide riboside transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913783,7570AC99.4% 546.3 / ‑4.1 153I198L (ATT→CTT) pnuCnicotinamide riboside transporter
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (68/84);  total (68/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

Only 100 of 153 total aligned reads displayed.

TGATGGTGTTATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTATTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCAC  >  NC_000913/783662‑783853
                                                                                               |                                                                                                
tgatgGTGTTATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCtttt                                                                                              >  2:3699500/1‑100 (MQ=255)
 gatgGTGTTATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCttttt                                                                                             <  1:1737809/100‑1 (MQ=255)
  atgGTGTTATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTg                                                                                            <  2:1371166/100‑1 (MQ=255)
  atgGTGTTATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTg                                                                                            <  2:1505327/100‑1 (MQ=255)
   tgGTGTTATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGc                                                                                           >  1:2542551/1‑100 (MQ=255)
   tgGTGTTATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGc                                                                                           <  2:707868/100‑1 (MQ=255)
    ggTGTTATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCa                                                                                          <  1:1702938/100‑1 (MQ=255)
     gtgtTATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCAc                                                                                         <  1:45964/100‑1 (MQ=255)
     gtgtTATCTATCGTGGCAAAGATTCTGATGACGCGTAAGTATGTGGACAACTGGCTGTTGTGGGTGATTGTTAACGTGAATATCGTCGTTCTTTTTGCAc                                                                                         >  2:3674225/1‑100 (MQ=255)
      tgtTATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACt                                                                                        <  2:2319530/100‑1 (MQ=255)
       gtTATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACtt                                                                                       >  1:3925380/1‑100 (MQ=255)
        ttATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTc                                                                                      >  1:1870550/1‑100 (MQ=255)
         tatctatcGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCagatcg                                                                                     >  2:2993596/1‑95 (MQ=255)
         tatctatcGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCa                                                                                     >  1:1618114/1‑100 (MQ=255)
         tatctatcGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCa                                                                                     <  1:459425/100‑1 (MQ=255)
            ctatcGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAggg                                                                                  >  2:3318112/1‑100 (MQ=255)
            ctatcGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAggg                                                                                  >  2:1857984/1‑100 (MQ=255)
            ctatcGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAggg                                                                                  <  2:172880/100‑1 (MQ=255)
            ctatcGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAggg                                                                                  >  1:1210243/1‑100 (MQ=255)
              atcGTGGCAATGATTCTGATGACGCGGAAGTATGTGTAAAACTGTCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTTCACTTCAGGGCg                                                                                <  1:3066190/100‑1 (MQ=255)
                cGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGtt                                                                              >  2:1894775/1‑100 (MQ=255)
                  tGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTa                                                                            <  1:2257153/100‑1 (MQ=255)
                   ggCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTAc                                                                           >  2:2620287/1‑100 (MQ=255)
                    gCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTATTGGGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACg                                                                          <  2:323126/100‑1 (MQ=255)
                     cAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGc                                                                         >  2:1956918/1‑100 (MQ=255)
                      agtGATTCTGATGACGCGTAAGTATGGGGAAAACTGGTTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGcc                                                                        <  2:3874826/98‑1 (MQ=255)
                      aaTGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGcc                                                                        <  1:2022954/100‑1 (MQ=255)
                       aTGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCa                                                                       <  2:3542046/100‑1 (MQ=255)
                       aTGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCa                                                                       >  1:1366769/1‑100 (MQ=255)
                         gATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATg                                                                     >  1:1309971/1‑100 (MQ=255)
                         gATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATg                                                                     >  2:2496949/1‑100 (MQ=255)
                             cTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATgagat                                                                 >  1:3647994/1‑96 (MQ=255)
                              tgatgaCGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTctct                                                                <  1:2898746/100‑1 (MQ=255)
                              tgatgaCGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTctct                                                                >  2:2159822/1‑100 (MQ=255)
                                atgaCGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTgg                                                              <  1:3705523/100‑1 (MQ=255)
                                 tgaCGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGa                                                             <  2:321363/100‑1 (MQ=255)
                                 tgaCGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGa                                                             >  1:4082391/1‑100 (MQ=255)
                                  gaCGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAg                                                            <  2:784148/100‑1 (MQ=255)
                                   aCGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGt                                                           <  1:297429/100‑1 (MQ=255)
                                    cgcgTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTa                                                          <  1:3541003/100‑1 (MQ=255)
                                    cgcgTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTa                                                          >  1:922867/1‑100 (MQ=255)
                                     gcgTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTAc                                                         <  2:2219329/100‑1 (MQ=255)
                                     gcgTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTAc                                                         <  1:930739/100‑1 (MQ=255)
                                      cgTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGATTAca                                                        >  2:3498407/1‑100 (MQ=255)
                                      cgTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTAca                                                        >  1:3427073/1‑100 (MQ=255)
                                       gTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTAcat                                                       <  2:575546/100‑1 (MQ=255)
                                        tAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTAcatc                                                      <  2:2060470/100‑1 (MQ=255)
                                         aaGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTAcatca                                                     <  2:3424667/100‑1 (MQ=255)
                                         aaGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTAcatca                                                     <  2:2805762/100‑1 (MQ=255)
                                          aGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTTTTTTTTTTTGTGTGTACGCAATGTCTCTGGATTActtctt                                                    >  2:3550529/1‑98 (MQ=255)
                                          aGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTAcatcat                                                    <  1:580029/100‑1 (MQ=255)
                                              tgtgGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTg                                                >  2:2795697/1‑100 (MQ=255)
                                               gtgGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGa                                               >  2:406049/1‑100 (MQ=255)
                                                tgGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGAc                                              >  2:2464481/1‑100 (MQ=255)
                                                 ggAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGAcc                                             >  2:3807835/1‑100 (MQ=255)
                                                   aaaaCTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCtt                                           <  1:1134768/100‑1 (MQ=255)
                                                   aaaaCTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCtt                                           >  1:1478784/1‑100 (MQ=255)
                                                    aaaCTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCttt                                          <  1:2472177/100‑1 (MQ=255)
                                                     aaCTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTa                                         <  2:26259/100‑1 (MQ=255)
                                                     aaCTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCAACCTGACCTTTa                                         <  2:1226715/100‑1 (MQ=255)
                                                      aCTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTAt                                        >  2:3720415/1‑100 (MQ=255)
                                                       cTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTAtt                                       <  1:3274141/100‑1 (MQ=255)
                                                           cTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTgcgc                                   <  1:3988719/100‑1 (MQ=255)
                                                           cTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTgcgc                                   <  1:3592368/100‑1 (MQ=255)
                                                            tgttgtGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCt                                  <  2:2503504/100‑1 (MQ=255)
                                                            tgttgtGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCt                                  <  1:2179938/100‑1 (MQ=255)
                                                            tgttgtGGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCt                                  <  1:2542737/100‑1 (MQ=255)
                                                              ttgtGGGTGATTATTAACGTGATTCGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCa                                <  2:1415189/100‑1 (MQ=255)
                                                               ngtgGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCaa                               >  2:1691808/2‑100 (MQ=255)
                                                                gtgGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAAc                              <  1:823492/100‑1 (MQ=255)
                                                                gtgGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTATATTGCGCTCAAc                              <  2:1523239/100‑1 (MQ=255)
                                                                 tgGGTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACg                             >  2:747625/1‑100 (MQ=255)
                                                                  gggTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACgg                            <  1:763599/100‑1 (MQ=255)
                                                                  gggTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACgg                            >  1:3056224/1‑100 (MQ=255)
                                                                   ggTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGc                           >  1:2817770/1‑100 (MQ=255)
                                                                    gTGATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCa                          <  1:34695/100‑1 (MQ=255)
                                                                      gATTATTAACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGc                        >  2:3290380/1‑100 (MQ=255)
                                                                         tattaACGTGATTAGCGTCGTTCTTTTTTTTTTTTTTTTCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCgg                     >  2:901498/1‑100 (MQ=255)
                                                                         tattaACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCgg                     >  1:3287608/1‑100 (MQ=255)
                                                                         tattaACGTGATTAGCGTCGTTCTCTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCgg                     <  2:1088923/100‑1 (MQ=255)
                                                                          attaACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGa                    >  1:3672278/1‑100 (MQ=255)
                                                                           ttaACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGAt                   >  1:1325677/1‑100 (MQ=255)
                                                                            taACGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGAtg                  <  2:1301592/100‑1 (MQ=255)
                                                                              aCGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGAtgtg                >  1:814574/1‑100 (MQ=255)
                                                                              aCGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGAtgtg                <  2:540667/100‑1 (MQ=255)
                                                                              aCGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGAtgtg                <  1:3800732/100‑1 (MQ=255)
                                                                               cGTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTgg               <  1:959719/100‑1 (MQ=255)
                                                                                gTGATTAGCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGa              <  2:3843542/100‑1 (MQ=255)
                                                                                 tGATTATCGGCGTGCTTTTTGCACTTCGGGGCTTTTTCGCCAGGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCGACGGCAGCCGGATGTGGAt             <  2:786568/100‑1 (MQ=255)
                                                                                  gATTAGCGTCGTTCTTTTTGAACTTTTGGGGGTGTACGCCTTGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATc            <  2:2366692/100‑1 (MQ=255)
                                                                                       gCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAg       <  1:2229964/100‑1 (MQ=255)
                                                                                       gCGTCGTTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAg       <  1:1030689/100‑1 (MQ=255)
                                                                                        cgtcgtTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATAAACAgc      >  2:3889249/1‑100 (MQ=255)
                                                                                        cgtcgtTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACACCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAgc      <  2:395185/100‑1 (MQ=255)
                                                                                         gtcgtTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAgcg     >  1:4011686/1‑100 (MQ=255)
                                                                                           cgtTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCa   <  1:384345/100‑1 (MQ=255)
                                                                                           cgtTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCa   <  1:870028/100‑1 (MQ=255)
                                                                                            gtTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCAc  >  1:2666474/1‑100 (MQ=255)
                                                                                            gtTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCAc  >  1:1635887/1‑100 (MQ=255)
                                                                                            gtTCTTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCAc  >  2:2979979/1‑100 (MQ=255)
                                                                                               |                                                                                                
TGATGGTGTTATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTTGTGGGTGATTATTAACGTGATTAGCGTCGTTATTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCAC  >  NC_000913/783662‑783853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A