Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F412 I3 R1 53 201.3 10250884 92.1% 9441064 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,451,758 A→G Q234R (CAG→CGG)  ytfR → putative sugar ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,451,7580AG99.4% 582.9 / NA 163Q234R (CAG→CGG) ytfRputative sugar ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (90/72);  minor base C (0/1);  total (90/73)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 165 total aligned reads displayed.

CTCACTTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCAGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAACCCGTTGCCGC  >  NC_000913/4451662‑4451854
                                                                                                |                                                                                                
ctcactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGCGCTACCGCGGAt                                                                                               >  2:3776869/1‑100 (MQ=255)
ctcactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGAt                                                                                               >  1:2742737/1‑100 (MQ=255)
 tcactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATc                                                                                              <  1:3200776/100‑1 (MQ=255)
  cactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCg                                                                                             <  1:793034/100‑1 (MQ=255)
      ttCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACt                                                                                         <  1:2962187/100‑1 (MQ=255)
       tctcGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTg                                                                                        <  1:2911191/100‑1 (MQ=255)
        ctcGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTgg                                                                                       >  2:92142/1‑100 (MQ=255)
         tcGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGTCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGt                                                                                      <  2:4598404/100‑1 (MQ=255)
          cGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTgaga                                                                                     >  2:2878698/1‑97 (MQ=255)
           gATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTaa                                                                                    >  1:1530051/1‑100 (MQ=255)
           gATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTaa                                                                                    >  2:2966860/1‑100 (MQ=255)
                ggTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAatg                                                                               >  1:4838604/1‑100 (MQ=255)
                ggTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAatg                                                                               >  1:4033445/1‑100 (MQ=255)
                ggTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAatg                                                                               >  1:1307284/1‑100 (MQ=255)
                     aTCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGTAAACGTTCTAGCTACCGCGGATCGAACTGGGAAAAATGATGCt                                                                          >  2:230175/1‑100 (MQ=255)
                     aTCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCt                                                                          <  1:1459661/100‑1 (MQ=255)
                       cAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTgg                                                                        <  1:523693/100‑1 (MQ=255)
                       cAGGGCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGTTCGAACTGGTAAAAATGATGCTgg                                                                        <  2:3726361/100‑1 (MQ=255)
                        aGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTggg                                                                       <  2:663006/100‑1 (MQ=255)
                        aGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTggg                                                                       >  1:1254244/1‑100 (MQ=255)
                         ggTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTgggg                                                                      >  2:1563226/1‑100 (MQ=255)
                          gTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGgc                                                                     >  2:2628113/1‑100 (MQ=255)
                          gTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGgc                                                                     >  1:4357545/1‑100 (MQ=255)
                           tCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGgcg                                                                    <  1:3566616/100‑1 (MQ=255)
                             aGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGcgcg                                                                  <  1:939807/100‑1 (MQ=255)
                              gCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGACCGAACGGGTAAAAATGATGCTGGGGCGCGa                                                                 >  2:818898/1‑100 (MQ=255)
                               cGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAg                                                                >  2:3385767/1‑100 (MQ=255)
                               cGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAg                                                                <  1:1674562/100‑1 (MQ=255)
                                  tCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTg                                                             <  2:2637381/100‑1 (MQ=255)
                                   cGGATCACCGTCTTACGCAACGGCAGTTGCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTgg                                                            >  2:419478/1‑100 (MQ=255)
                                    ggATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGa                                                           >  1:1312775/1‑100 (MQ=255)
                                    ggATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGa                                                           >  1:831674/1‑100 (MQ=255)
                                     gATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGAt                                                          <  2:1114773/100‑1 (MQ=255)
                                     gATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGAt                                                          >  1:699433/1‑100 (MQ=255)
                                      aTCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATa                                                         <  1:2268849/100‑1 (MQ=255)
                                      aTCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAACTGGATa                                                         <  1:3040851/100‑1 (MQ=255)
                                       tCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATAc                                                        <  2:19163/100‑1 (MQ=255)
                                       tCACCGTCTTACGCAACGGCAGTGTCGTAGGCTGTCGGGAAACGTTCGGGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATAc                                                        <  2:3996138/100‑1 (MQ=255)
                                        cACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATAcc                                                       <  1:2812706/100‑1 (MQ=255)
                                         aCCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATAccc                                                      >  2:469872/1‑100 (MQ=255)
                                          ccGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCa                                                     <  2:4292646/100‑1 (MQ=255)
                                            gTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCAcg                                                   <  2:2904169/100‑1 (MQ=255)
                                            gTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCAcg                                                   >  1:3809204/1‑100 (MQ=255)
                                             tCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCAcgc                                                  <  1:440958/100‑1 (MQ=255)
                                              cTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCAcgcg                                                 <  1:3423603/100‑1 (MQ=255)
                                               ttACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACgcgc                                                <  2:2705951/100‑1 (MQ=255)
                                               ttACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACgcgc                                                <  2:2277955/100‑1 (MQ=255)
                                                tACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCGACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCt                                               >  1:41371/1‑100 (MQ=255)
                                                  cGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTAc                                             <  1:1389721/100‑1 (MQ=255)
                                                  cGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTAc                                             >  1:1109531/1‑100 (MQ=255)
                                                    cAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAg                                           <  2:409576/100‑1 (MQ=255)
                                                    cAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAg                                           >  2:3347109/1‑100 (MQ=255)
                                                     aaCGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGc                                          <  1:3474424/100‑1 (MQ=255)
                                                      aCGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCg                                         >  1:309887/1‑100 (MQ=255)
                                                       cGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGt                                        >  1:4632473/1‑100 (MQ=255)
                                                         gCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATCCCCACGCGCTACAGCGTGc                                      >  2:4535896/1‑100 (MQ=255)
                                                         gCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCAGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGc                                      >  1:4862092/1‑100 (MQ=255)
                                                          cAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGcc                                     >  2:5030233/1‑100 (MQ=255)
                                                          cAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGcc                                     >  1:2379501/1‑100 (MQ=255)
                                                           aGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCg                                    <  2:1722298/100‑1 (MQ=255)
                                                             tttCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCggg                                  <  1:5053084/100‑1 (MQ=255)
                                                              ttCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGc                                 <  1:3311569/100‑1 (MQ=255)
                                                              ttCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGc                                 <  2:3427035/100‑1 (MQ=255)
                                                                 gTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGaa                              >  1:2482635/1‑100 (MQ=255)
                                                                 gTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGaa                              >  2:1693958/1‑100 (MQ=255)
                                                                  tAGGCTGTCGGGAAACGTGCGAGCTACCGCGGCTCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAAc                             >  2:4572184/1‑100 (MQ=255)
                                                                   aGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACa                            >  1:3758148/1‑100 (MQ=255)
                                                                   aGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACa                            <  1:3218483/100‑1 (MQ=255)
                                                                      cTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACAttg                         >  2:1282541/1‑100 (MQ=255)
                                                                      cTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACAttg                         >  1:1876147/1‑100 (MQ=255)
                                                                       tGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACAttgt                        <  2:2581674/100‑1 (MQ=255)
                                                                         tCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACAttgttg                      <  2:1234520/100‑1 (MQ=255)
                                                                          cGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGa                     >  2:1459769/1‑100 (MQ=255)
                                                                          cGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGa                     <  2:4437237/100‑1 (MQ=255)
                                                                          cGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGa                     >  1:2119391/1‑100 (MQ=255)
                                                                          cGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGa                     <  1:655284/100‑1 (MQ=255)
                                                                          cGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGa                     >  2:523182/1‑100 (MQ=255)
                                                                          cGGAAAACGTGCGAGCTACCGCGGATCGAACAGGAAAAAGTGATGCTGGTGTGCGTGCTGGATACCCACGTCCTACAGCGTCTCGGGCGAACTTTGCTGa                     >  2:1056409/1‑100 (MQ=255)
                                                                           gggAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAg                    <  2:3911869/100‑1 (MQ=255)
                                                                            ggAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGc                   >  1:2171826/1‑100 (MQ=255)
                                                                            ggAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGc                   <  2:4702650/100‑1 (MQ=255)
                                                                             gAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCg                  <  2:2093459/100‑1 (MQ=255)
                                                                               aaCGTGCGCGCTACCGCCGATAGAACTGGTAAAAATGATGCCGGGGCGCCAGCGGGCTAACCACGCGCGACAGCTTGCCTGTCCAACATTGTTGCGCGat                <  2:1528798/100‑2 (MQ=255)
                                                                               aaCGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGAc                <  1:425265/100‑1 (MQ=255)
                                                                               aaCGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGAc                <  2:754232/100‑1 (MQ=255)
                                                                               aaCGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGAc                >  1:4054439/1‑100 (MQ=255)
                                                                                 cGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACaa              <  1:1923238/100‑1 (MQ=255)
                                                                                   tGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAAc            <  1:1771248/100‑1 (MQ=255)
                                                                                        gCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAACCCGtt       >  2:3202964/1‑100 (MQ=255)
                                                                                         cTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTTGATACCCACGCGCTACCGCGTGCCGGGCGAACATTGTTGAGCGACAACCCCGTTg      <  2:3633166/100‑1 (MQ=255)
                                                                                         cTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAACCCGTTg      >  2:1370587/1‑100 (MQ=255)
                                                                                         cTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAACCCGTTg      >  2:2017152/1‑100 (MQ=255)
                                                                                         cTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAACCCGTTg      >  1:834577/1‑100 (MQ=255)
                                                                                          tACCGCGGATCGAACGGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACATCGTGCCGGGCGAACATTGTTGAGCGACAAACCCGTTGc     >  2:1022792/1‑100 (MQ=255)
                                                                                           aCCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAACCCGTTGcc    >  2:2916707/1‑100 (MQ=255)
                                                                                            ccGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAACCCGTTGCcg   <  2:2874548/100‑1 (MQ=255)
                                                                                            ccGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAACCCGTTGCcg   >  2:3660139/1‑100 (MQ=255)
                                                                                            ccGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGACTGGCCAACACTGTTGAGCGCCAAACCCGTTGcag   >  2:4265670/1‑98 (MQ=255)
                                                                                             cgcgGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAACCCGTTGCcgc  >  2:4795060/1‑100 (MQ=255)
                                                                                             cgcgGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAACCCGTTGCcgc  >  1:5155220/1‑100 (MQ=255)
                                                                                                |                                                                                                
CTCACTTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCAGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAACCCGTTGCCGC  >  NC_000913/4451662‑4451854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A