Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F169 I1 R1 62 44.4 970160 98.1% 951726 147.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 536,758 C→T P202L (CCA→CTA)  rplY → 50S ribosomal protein L25

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE536,7580CT100.0% 207.6 / NA 62P202L (CCA→CTA) rplY50S ribosomal protein L25
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/43);  total (19/43)

GTTGCTGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACCAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTTGTGTTATTATAAAGCT  >  USA300TCH1516_ALE/536619‑536902
                                                                                                                                           |                                                                                                                                                
gTTGCTGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAgttg                                                                                                                                          >  4:294662/1‑148 (MQ=255)
     tGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGc                                                                                                                                     <  4:376559/148‑1 (MQ=255)
         gTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGaaa                                                                                                                                 >  3:334787/1‑148 (MQ=255)
         gTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGaaa                                                                                                                                 <  3:160803/148‑1 (MQ=255)
              aGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCaaa                                                                                                                            >  4:154196/1‑148 (MQ=255)
                     ggCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGTTCCTACTGAAGAAGCAACCGCAGCGATGGACGCCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACg                                                                                                                     <  4:340086/148‑1 (MQ=255)
                       cGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACgaa                                                                                                                   <  3:54090/148‑1 (MQ=255)
                       cGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACgaa                                                                                                                   <  3:413303/148‑1 (MQ=255)
                            tCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGaaaa                                                                                                              <  3:349881/148‑1 (MQ=255)
                            tCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGaaaa                                                                                                              <  3:348296/148‑1 (MQ=255)
                             cAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACGGAAGAAGAAAGCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTGGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGaaaaa                                                                                                             >  3:208366/1‑148 (MQ=255)
                                       aaCGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGt                                                                                                   >  4:109217/1‑148 (MQ=255)
                                       aaCGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAAATGAAGAGt                                                                                                   >  4:379989/1‑148 (MQ=255)
                                             tCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAagag                                                                                                    <  1:39334/141‑1 (MQ=255)
                                             tCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAAtttt                                                                                             <  3:308548/148‑1 (MQ=255)
                                                gCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAAt                                                                                          >  4:36615/1‑148 (MQ=255)
                                                gCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAAt                                                                                          >  4:273868/1‑148 (MQ=255)
                                                   gAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTg                                                                                       <  4:77402/148‑1 (MQ=255)
                                                    aaTCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGt                                                                                      <  3:6082/148‑1 (MQ=255)
                                                       cAGGAGTAACAGTAGTTGCTCCAACTGCAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAAt                                                                                          <  2:39186/141‑1 (MQ=255)
                                                          tagtaACAGTAGTTGCTCCAACTGAAGAACCAACTG‑AGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTa                                                                               <  3:102342/148‑1 (MQ=255)
                                                           agtaACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGATGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGt                                                                                      <  1:53546/141‑1 (MQ=255)
                                                           agtaACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTa                                                                               <  3:278338/148‑1 (MQ=255)
                                                           agtaACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTa                                                                               <  3:201158/148‑1 (MQ=255)
                                                           agtaACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTa                                                                               <  3:99175/148‑1 (MQ=255)
                                                            gtaACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTaa                                                                              >  3:406993/1‑148 (MQ=255)
                                                                cAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGtt                                                                          <  3:238645/148‑1 (MQ=255)
                                                                cAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGtt                                                                          <  3:83366/148‑1 (MQ=255)
                                                                cAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGtt                                                                          <  3:349943/148‑1 (MQ=255)
                                                                aagtagtTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACAt                                                                                 <  2:47878/140‑1 (MQ=255)
                                                                 agtagtTGTTGCAACTGAAGAAACAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAAAAGAAAAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGttt                                                                         <  4:411383/148‑1 (MQ=255)
                                                                 agtagtTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAGGttt                                                                         <  3:326414/148‑1 (MQ=255)
                                                                 agtagtTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGttt                                                                         >  3:99358/1‑148 (MQ=255)
                                                                 agtagtTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGttt                                                                         <  4:276704/148‑1 (MQ=255)
                                                                 agtagtTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGttt                                                                         <  3:401769/148‑1 (MQ=255)
                                                                 agtagtTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGttt                                                                         >  3:275660/1‑148 (MQ=255)
                                                                 agtagtTACTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGttt                                                                         <  4:114143/148‑1 (MQ=255)
                                                                     gtTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAGAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTtat                                                                     <  4:85790/148‑1 (MQ=255)
                                                                       tGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATAc                                                                   >  3:100017/1‑148 (MQ=255)
                                                                          tCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACttt                                                                <  3:163931/148‑1 (MQ=255)
                                                                           ccaactgaagaaccaactgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTta                                                                      <  2:5000/141‑1 (MQ=255)
                                                                           ccaactgaagaaccaactgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTg                                                               >  4:215008/1‑148 (MQ=255)
                                                                                          actgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACtgt                                                <  3:134033/148‑1 (MQ=255)
                                                                                          actgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACtgt                                                <  3:249206/148‑1 (MQ=255)
                                                                                          actgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAAAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGACGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACaa                                                       <  1:53552/141‑1 (MQ=255)
                                                                                            tgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAATAAAGATCTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGc                                              <  4:369391/148‑1 (MQ=255)
                                                                                            tgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGc                                                     >  1:27067/1‑141 (MQ=255)
                                                                                            tgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGc                                              <  3:277488/148‑1 (MQ=255)
                                                                                            tgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGc                                              <  4:229269/148‑1 (MQ=255)
                                                                                            tgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGc                                              >  4:237341/1‑148 (MQ=255)
                                                                                              aagaagaaATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAAAAAAAAAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCtt                                            <  4:95386/148‑1 (MQ=255)
                                                                                                    gaaggtgaGCTATGGGAGGCGAAGAGGAAGCTGGAGGACTGGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCt                                             <  2:17043/134‑1 (MQ=255)
                                                                                                    aaaTCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTa                                      <  3:27922/148‑1 (MQ=255)
                                                                                                                 ggAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGt                         <  3:377331/148‑1 (MQ=255)
                                                                                                                 ggAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGt                         >  3:331461/1‑148 (MQ=255)
                                                                                                                 ggAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGt                         <  3:294662/148‑1 (MQ=255)
                                                                                                                          acaacaAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATGTAAGCATGGTGCTTTTtgt                <  3:273868/148‑1 (MQ=255)
                                                                                                                          acaacaAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTtgt                <  3:36615/148‑1 (MQ=255)
                                                                                                                                 aCTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTTGTGttatta         >  4:136863/1‑148 (MQ=255)
                                                                                                                                 aCTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTTGTGttatta         >  4:373918/1‑148 (MQ=255)
                                                                                                                                 aCTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTATTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTTGTGttatta         >  4:344233/1‑148 (MQ=255)
                                                                                                                                        aaCTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTTGTGTTATTATAAAGCt  <  4:148176/148‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                
GTTGCTGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACCAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTTGTGTTATTATAAAGCT  >  USA300TCH1516_ALE/536619‑536902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A