breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation annotation gene description
RA CP000731 81 N→G intergenic (–/‑151)  / → repA –/replication protein A
JC CP000731 6,552 +28 bp intergenic (+1069/‑419) cadX → / → USA300HOU_pUSA300HOUMR0010 cadmium efflux regulator/MarR family transcriptional regulator
RA CP000731 8,740 T→C I8V (ATT→GTT)  blaZ ← beta‑lactamase
RA CP000731 25,669 (A)7→6 coding (322/333 nt) USA300HOU_pUSA300HOUMR0031 → hypothetical protein
RA USA300TCH1516_ALE 39,195 T→C K647E (AAA→GAA)  pbp ← Beta‑lactam‑inducible penicillin‑binding protein
RA USA300TCH1516_ALE 39,378 C→T D586N (GAT→AAT)  pbp ← Beta‑lactam‑inducible penicillin‑binding protein
RA USA300TCH1516_ALE 51,334 (G)7→8 intergenic (‑132/‑7) USA300HOU_RS00210 ← / → USA300HOU_RS00215 hypothetical protein/hypothetical protein
RA USA300TCH1516_ALE 76,969 G→A intergenic (+509/‑107) USA300HOU_RS00140 → / → USA300HOU_RS00350 hypothetical protein/Monoacylglycerol lipase
RA USA300TCH1516_ALE 93,268 G→A A73T (GCT→ACT)  glpE → Thiosulfate sulfurtransferase GlpE
RA USA300TCH1516_ALE 129,796 G→C intergenic (‑209/+212) spa ← / ← sarS Immunoglobulin G‑binding protein A/HTH‑type transcriptional regulator SarS
RA USA300TCH1516_ALE 169,407 G→A G82E (GGA→GAA)  repE → Replication initiation protein
RA USA300TCH1516_ALE 195,286 T→A intergenic (‑97/‑82) USA300HOU_RS00900 ← / → USA300HOU_RS00905 hypothetical protein/hypothetical protein
RA USA300TCH1516_ALE 266,981 A→G P228P (CCA→CCG USA300HOU_RS01175 → Staphylocoagulase
RA USA300TCH1516_ALE 396,540 G→A A208T (GCA→ACA)  efeM → putative iron uptake system component EfeM
RA USA300TCH1516_ALE 505,514 C→T A469V (GCG→GTG)  treP_1 → PTS system trehalose‑specific EIIBC component
RA USA300TCH1516_ALE 536,758 C→T P202L (CCA→CTA)  rplY → 50S ribosomal protein L25
RA USA300TCH1516_ALE 607,884 G→A R209* (CGA→TGA)  dck ← Deoxyadenosine/deoxycytidine kinase
RA USA300TCH1516_ALE 629,681 C→A L84I (CTA→ATA)  USA300HOU_RS02990 → 3‑hexulose‑6‑phosphate synthase
RA USA300TCH1516_ALE 665,392 T→G L79* (TTA→TGA)  paiA_1 → Spermidine/spermine N(1)‑acetyltransferase
RA USA300TCH1516_ALE 707,910 A→G intergenic (‑1/‑121) znuC_1 ← / → ideR High‑affinity zinc uptake system ATP‑binding protein ZnuC/Iron‑dependent repressor IdeR
RA USA300TCH1516_ALE 744,375 A→T I506F (ATT→TTT)  melR_1 → Melibiose operon regulatory protein
RA USA300TCH1516_ALE 749,249 C→T S59S (AGC→AGT setC → Sugar efflux transporter C
RA USA300TCH1516_ALE 890,260 T→C V39A (GTT→GCT)  gcvH → Glycine cleavage system H protein
RA USA300TCH1516_ALE 1,017,678 T→C F391S (TTC→TCC)  acp → Sodium/proton‑dependent alanine carrier protein
RA USA300TCH1516_ALE 1,063,912 +C coding (577/1218 nt) ywtF ← Putative transcriptional regulator YwtF
RA USA300TCH1516_ALE 1,125,066 G→C E729Q (GAG→CAG)  cfiB_1 → 2‑oxoglutarate carboxylase small subunit
RA USA300TCH1516_ALE 1,163,829 T→C V72A (GTG→GCG)  USA300HOU_RS05765 → Putative metallophosphoesterase MG207
RA USA300TCH1516_ALE 1,233,504 T→A V470D (GTT→GAT)  prkC → Serine/threonine‑protein kinase PrkC
RA USA300TCH1516_ALE 1,298,108 T→A intergenic (+4/‑232) pnp → / → rnj2 Polyribonucleotide nucleotidyltransferase/Ribonuclease J 2
RA USA300TCH1516_ALE 1,324,256 (A)9→8 coding (1028/2010 nt) mutL → DNA mismatch repair protein MutL
RA USA300TCH1516_ALE 1,358,236 A→G G300G (GGA→GGG hom → Homoserine dehydrogenase
RA USA300TCH1516_ALE 1,416,458 A→T I216N (ATT→AAT)  oppD_4 ← Oligopeptide transport ATP‑binding protein OppD
RA USA300TCH1516_ALE 1,440,085 G→T intergenic (‑45/+126) cspA_2 ← / ← USA300TCH1516_01338 Cold shock protein CspA/hypothetical protein
RA USA300TCH1516_ALE 1,499,376 C→A G883V (GGT→GTT)  ebh_1 ← Extracellular matrix‑binding protein ebh
RA USA300TCH1516_ALE 1,513,930 A→T L413F (TTA→TTT USA300HOU_RS07375 → hypothetical protein
RA USA300TCH1516_ALE 1,613,762 T→C intergenic (‑31/‑37) scpA ← / → USA300HOU_RS08000 Segregation and condensation protein A/hypothetical protein
RA USA300TCH1516_ALE 1,624,673 T→A K192I (AAA→ATA)  malL ← Oligo‑1,6‑glucosidase
RA USA300TCH1516_ALE 1,646,189 G→C A155G (GCA→GGA)  ypdF ← Aminopeptidase YpdF
RA USA300TCH1516_ALE 1,744,362 G→A H104Y (CAC→TAC)  apt ← Adenine phosphoribosyltransferase
RA USA300TCH1516_ALE 1,761,889 C→A D388Y (GAT→TAT)  fpgS ← Folylpolyglutamate synthase
RA USA300TCH1516_ALE 1,769,572 A→G V295V (GTT→GTC hemB ← Delta‑aminolevulinic acid dehydratase
RA USA300TCH1516_ALE 1,830,883 C→T K79K (AAG→AAA ezrA ← Septation ring formation regulator EzrA
RA USA300TCH1516_ALE 1,851,260 C→A A115S (GCG→TCG)  acsA_1 ← Acetyl‑coenzyme A synthetase
RA USA300TCH1516_ALE 1,932,853 T→C I799V (ATT→GTT)  USA300HOU_RS09605 ← hypothetical protein
RA USA300TCH1516_ALE 1,963,768 C→G V365L (GTA→CTA)  hemY ← Protoporphyrinogen oxidase
RA USA300TCH1516_ALE 2,004,062 (A)8→7 intergenic (‑2285/+99) USA300TCH1516_01884 ← / ← perR tRNA‑Ile/Peroxide‑responsive repressor PerR
RA USA300TCH1516_ALE 2,010,363 C→T C146Y (TGT→TAT)  USA300HOU_RS10125 ← Putative multidrug export ATP‑binding/permease protein
RA USA300TCH1516_ALE 2,018,612 G→A R103* (CGA→TGA)  mgt ← Monofunctional glycosyltransferase
RA USA300TCH1516_ALE 2,078,573 (T)7→8 coding (246/681 nt) USA300HOU_RS10490 ← hypothetical protein
RA USA300TCH1516_ALE 2,079,114 T→C H201R (CAC→CGC)  natA_1 ← ABC transporter ATP‑binding protein NatA
RA USA300TCH1516_ALE 2,086,905 (A)8→7 intergenic (‑56/‑629) scn_3 ← / → chp Staphylococcal complement inhibitor/Chemotaxis inhibitory protein
RA USA300TCH1516_ALE 2,272,733 C→T intergenic (‑339/‑342) glmS ← / → mtlA Glutamine‑‑fructose‑6‑phosphate aminotransferase [isomerizing]/PTS system mannitol‑specific EIICB component
RA USA300TCH1516_ALE 2,279,705 G→A A943V (GCA→GTA)  ebh_3 ← Extracellular matrix‑binding protein ebh
RA USA300TCH1516_ALE 2,352,815 G→A A92V (GCA→GTA)  rpsI ← 30S ribosomal protein S9
RA USA300TCH1516_ALE 2,454,626 A→T L48L (CTA→CTT lyrA → Lysostaphin resistance protein A
RA USA300TCH1516_ALE 2,502,887 (A)5→6 coding (1411/2106 nt) melR_2 → Melibiose operon regulatory protein
RA USA300TCH1516_ALE 2,532,298 A→T T15T (ACA→ACT femA_3 → Aminoacyltransferase FemA
RA USA300TCH1516_ALE 2,543,088 G→A R283K (AGA→AAA)  hlgA → Gamma‑hemolysin component A
RA USA300TCH1516_ALE 2,679,462 (A)8→7 intergenic (+334/‑55) USA300HOU_RS13790 → / → ssaA2_4 hypothetical protein/Staphylococcal secretory antigen ssaA2
RA USA300TCH1516_ALE 2,709,444 (T)6→5 coding (1483/1812 nt) oatA_2 ← O‑acetyltransferase OatA
RA USA300TCH1516_ALE 2,750,562 T→C V197V (GTA→GTG betA ← Oxygen‑dependent choline dehydrogenase
RA USA300TCH1516_ALE 2,787,510 T→C intergenic (+56/‑184) zipA_3 → / → manR_2 Cell division protein ZipA/Transcriptional regulator ManR

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE 512620 =44 (1.000)34 (1.070)
+40 bp
22/212 0.2 51.6% intergenic (+688/‑6075) recR/speA_2 Recombination protein RecR/Arginine decarboxylase
?USA300TCH1516_ALE 1998278 = NA (NA)intergenic (‑6/+3329) USA300TCH1516_01882/USA300TCH1516_01883 tRNA‑Val/tRNA‑Ala