Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F109 I0 R1	76	110.5	2404748	96.5%	2320581	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	19,264	G→A	28.0%	G307D (GGC→GAC)	gdpP →	Cyclic‑di‑AMP phosphodiesterase GdpP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	19,264	0	G	A	28.0%	194.8 / 76.8	120	G307D (GGC→GAC)	gdpP	Cyclic‑di‑AMP phosphodiesterase GdpP
Reads supporting (aligned to +/- strand): ref base G (46/40); new base A (21/13); total (67/53)
Fisher's exact test for biased strand distribution p-value = 5.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

TATCGGTGTTGGTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTGCA  >  USA300TCH1516_ALE/19130‑19403

tATCGGTGTTGGTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTaa                                                                                                                                       <  1:812136/141‑1 (MQ=255)

   cGGTGTTGGTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGAc                                                                                                                                    >  2:1144246/1‑141 (MQ=255)

   cGGTGTTGGTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCAAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGATTCTATGGCGGTAAGAc                                                                                                                                    >  2:803004/1‑141 (MQ=255)

   cGGTGTTGGTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATCACCTTCAACAATTCTAGACTTCGAATGAGGACTCGGTGGCGACAAAGTTGCCATTAAATGTATTAATGGGGATGTGCGTTTCTATGGCGGTAAGAc                                                                                                                                    >  1:280622/1‑141 (MQ=255)

    ggTGTTGGTGTAGGTCCTGAAGATCGCCTCAACATAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTACTATGGCGGTAAGACt                                                                                                                                   >  2:846648/1‑141 (MQ=255)

      tgtTGGTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGAGCGTTTCTATGGCGGTAAGACTGa                                                                                                                                 >  2:748299/1‑141 (MQ=255)

      ggtTGGTGAAGGTACTGAAAATTTAATCGACTTAGGTAAAGTAACACAAGCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTTAAAGTATGACTGGTAAAGTGAGTTTCTATGGCGGAAAGAGTGa                                                                                                                                 >  2:227602/2‑141 (MQ=255)

         tGGTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAGTCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGGTTCTATGGCGGTAAGGCTGGccc                                                                                                                              >  2:1046380/1‑141 (MQ=255)

           gTGAAGGTTCTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTATGCTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGa                                                                                                                            <  1:244318/141‑1 (MQ=255)

           gTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGa                                                                                                                            <  1:1002062/141‑1 (MQ=255)

           gTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGa                                                                                                                            <  2:1094411/141‑1 (MQ=255)

           gTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGa                                                                                                                            <  2:316343/141‑1 (MQ=255)

           gTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGa                                                                                                                            >  1:444242/1‑141 (MQ=255)

           gTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGa                                                                                                                            >  2:667443/1‑141 (MQ=255)

           gTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGa                                                                                                                            <  1:1058368/141‑1 (MQ=255)

           gTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGa                                                                                                                            >  2:924205/1‑141 (MQ=255)

                       aaaaTTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTa                                                                                                                <  1:166335/141‑1 (MQ=255)

                       aaaaTTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTa                                                                                                                >  1:313350/1‑141 (MQ=255)

                       aaaaTTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTa                                                                                                                <  2:860988/141‑1 (MQ=255)

                        aaaTTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTAc                                                                                                               <  1:172787/141‑1 (MQ=255)

                            ttAATCGTGTTAGGTGAATTATGACAATCAGGCCTAGACTTAGCAGTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTGAGACTGACCCGATGGAGAAACGTACTCgt                                                                                                           <  2:582141/141‑1 (MQ=255)

                              aaTCGACTTAGGTGAATTATCACAATCCGGCCTAGACTTAGCATTAGGAAGCGGTGGCGACCAAGTTGAAATTAAAAGTATAAATGGTAATGTGCGTTTATATGAAGGCGAAACTGACCCGATGGAGAAAAGTaaacgcgt                                                                                                         >  1:836061/1‑134 (MQ=255)

                              aaTCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAAGGTGCGTTTCTATGACGGTAAGCCTGACCCGAGGGAGAAAGGTACTctggg                                                                                                         >  2:1114597/1‑137 (MQ=255)

                              aaTCGACTTAGGTGAATTATCAAAATCAGGCCTAAACTTAGCATTAGGACGCGGGGGCGAACAAGTTGCAATTAAAAGTATTAGTGGTAATGTGCGTTTCTATGACGGTAGAAATGACTCGATGTAGTAGCGTCATCgtga                                                                                                         >  2:707455/1‑140 (MQ=255)

                               aTCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTa                                                                                                        >  2:422723/1‑141 (MQ=255)

                                 cGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTTTAag                                                                                                      >  2:830993/1‑141 (MQ=255)

                                 cGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAag                                                                                                      >  2:334515/1‑141 (MQ=255)

                                   aCTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAagag                                                                                                    <  1:972345/141‑1 (MQ=255)

                                   aCTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAagag                                                                                                    >  2:613108/1‑141 (MQ=255)

                                     ttAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGAAGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAGACGTACTCGTGTAAGAGCa                                                                                                  >  2:233040/1‑141 (MQ=255)

                                       aGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAGTGGGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAAAGTCCTCGTGTAAGGACACg                                                                                                >  2:457510/1‑141 (MQ=255)

                                         gTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGGCCAAGTTGCAAGTAAAAGTATTAATGGTAATGGGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAAGGTACTCGTGTAAGGGCACgtg                                                                                              >  1:50141/1‑141 (MQ=255)

                                           gAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACgtggg                                                                                            >  1:1080122/1‑139 (MQ=255)

                                           gAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGtgtg                                                                                            <  1:982014/141‑1 (MQ=255)

                                           gAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGtgtg                                                                                            <  1:981943/141‑1 (MQ=255)

                                           gAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTGAAAGCACGtttg                                                                                            >  2:418531/1‑141 (MQ=255)

                                           gAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGtgtg                                                                                            >  2:433614/1‑141 (MQ=255)

                                                aTCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGAtctc                                                                                       >  2:900485/1‑141 (MQ=255)

                                                 tCACAATCAGGCCTAGACTTAGCATTAGGACGCGTAGGCGACGAATTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTca                                                                                      <  2:419793/141‑1 (MQ=255)

                                                  cacaATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAACAGTCTTAGTGGTAATGTGCGTTTCTCTGACGGTAAGACTGGCCCGACGGAGAAACGTAGTCGTGCGTGAGCAGGTGTGaggtcgc                                                                                     >  1:13455/1‑135 (MQ=255)

                                                  cacaATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTcac                                                                                     >  1:1035126/1‑141 (MQ=255)

                                                  cacaATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTcac                                                                                     >  1:478527/1‑141 (MQ=255)

                                                    caATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACAt                                                                                   <  2:280622/141‑1 (MQ=255)

                                                    caATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACAt                                                                                   <  1:542601/141‑1 (MQ=255)

                                                     aaTCAGGCCCAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATg                                                                                  <  1:757372/141‑1 (MQ=255)

                                                       tCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCGATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACAt                                                                                   >  2:652851/1‑138 (MQ=255)

                                                       tCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACAt                                                                                   <  1:652851/138‑1 (MQ=255)

                                                         aGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGtt                                                                              >  1:782241/1‑141 (MQ=255)

                                                           gacTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTaa                                                                            >  2:141301/3‑141 (MQ=255)

                                                           gCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTaa                                                                            >  1:708139/1‑141 (MQ=255)

                                                           gCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTaa                                                                            >  1:1196721/1‑141 (MQ=255)

                                                           gCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTaa                                                                            <  1:226907/141‑1 (MQ=255)

                                                                gACTGAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGat                                                                       <  1:526682/141‑1 (MQ=255)

                                                                    tAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATcc                                                                   >  2:1184972/1‑141 (MQ=255)

                                                                     aGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCt                                                                  <  1:141301/141‑1 (MQ=255)

                                                                      gCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGAGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCCCGTGTGATCTCACATGCGTCAAAAGATATCCtt                                                                 >  2:271367/1‑141 (MQ=255)

                                                                         ttAGGACGCGTGGGCGACCAAGTTGCAATTAAAAGTATTAAAGGTAATGTGCGTTTCTATGGCCGTAAGACTGACCCGATGTAGAACCGTCCTCGTGTAAGAGCACGTGTGATCTCAAATGCTTTAAAAGATCTGCTTgta                                                              <  2:635146/141‑3 (MQ=255)

                                                                         ttAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCa                                                              >  1:952935/1‑141 (MQ=255)

                                                                         ttAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCa                                                              >  1:185129/1‑141 (MQ=255)

                                                                         ttAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTACTAATGCTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCTTGTAAGAGCACGTGTGATCTAACATGCGTTAAATGATATCCATGCa                                                              <  1:843662/141‑1 (MQ=255)

                                                                            ggACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCcgag                                                           >  1:266266/1‑141 (MQ=255)

                                                                            ggACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCagag                                                           >  1:196990/1‑141 (MQ=255)

                                                                            ggACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTGTCAAAGGGGGTAAGAGTGTCCCGATGGTGACACGTATTCTTGTGAGAGCACGTGTGATCTAACATGCGTTATAAGATATCCTTGCagag                                                           >  2:299272/1‑141 (MQ=255)

                                                                              aCGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAggg                                                         >  2:319679/1‑141 (MQ=255)

                                                                               cgcgGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGt                                                        <  1:667443/141‑1 (MQ=255)

                                                                               cgcgGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAAGGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGt                                                        <  1:160693/141‑1 (MQ=255)

                                                                                    tGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACaa                                                   <  1:924205/141‑1 (MQ=255)

                                                                                     ggCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACaaa                                                  <  1:350543/141‑1 (MQ=255)

                                                                                     ggCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACaaa                                                  >  1:322839/1‑141 (MQ=255)

                                                                                      gCGACCAAGTTGCAATTAAAAGTATTCATGGTAATGGGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGGTCTCGCATGCGTTAAAAGATATCCTTGCAGAGGGGGGCAAAg                                                 >  2:564490/1‑141 (MQ=255)

                                                                                      gCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGGTCTCACATGCGTTCAAAGATATCCTTGCAGAGGGTGACAAAg                                                 >  2:120280/1‑141 (MQ=255)

                                                                                       ggACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCGGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGt                                                <  2:566314/140‑1 (MQ=255)

                                                                                       cGACCAAGTTGCAGTTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGt                                                <  2:860492/141‑1 (MQ=255)

                                                                                       cGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTGAGAGCACGTGTGATCTCACATGCGTTAAAAGATATGCTTGCAGAGGGTGACAAAGt                                                >  1:136463/1‑141 (MQ=255)

                                                                                       cGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGt                                                >  1:476745/1‑141 (MQ=255)

                                                                                       cGACCAAGTTGCAATTAAAAGTAGTAATGGTAATGTGCGTTTCTCTGGACGTGTGAGTGTCCCTAGCGAGAAACGTATTCGTGTTAGATCACGTGGGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGt                                                <  2:250829/141‑1 (MQ=255)

                                                                                          ccAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGCTCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCAt                                             <  2:150388/141‑1 (MQ=255)

                                                                                           cAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCAtt                                            <  1:545113/141‑1 (MQ=255)

                                                                                            aaGTTGCAATTAAAAGTATTAATGGTACGGTGCCTTTCTATGACGGTAAGACTGACCCGACGGAGAAACGTACTCGTGTAATAGCACGTGTGATCTCACATGCGTTAAAAGCTATCCTTGCTGAGGGTGACAAAGTCATTa                                           <  2:667087/141‑1 (MQ=255)

                                                                                                  cAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGGGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATgg                                     <  1:761901/141‑1 (MQ=255)

                                                                                                    aTTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGa                                   >  1:702097/1‑141 (MQ=255)

                                                                                                    aTTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGa                                   >  2:752478/1‑141 (MQ=255)

                                                                                                     ttAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGGTGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGGGACAAAGTCATTATCATGGGAc                                  <  2:342674/141‑1 (MQ=255)

                                                                                                      tAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCAGGGGACa                                 >  2:596715/1‑141 (MQ=255)

                                                                                                         aaGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATaa                              <  1:1184972/141‑1 (MQ=255)

                                                                                                         aaGTATTAATGGTAATGGGCGTTTCTATGGCGGTAAGCCTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATaa                              <  2:930427/141‑1 (MQ=255)

                                                                                                            tATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGCAACGTACGCTTGGAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACg                           >  1:1123648/1‑141 (MQ=255)

                                                                                                             aTTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACGTGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGt                          >  2:789661/1‑141 (MQ=255)

                                                                                                              ttAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTc                         >  1:802134/1‑141 (MQ=255)

                                                                                                                         gtgCGTTTCTATGGCGGTACGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGAt              >  1:634122/1‑141 (MQ=255)

                                                                                                                         gtgCGTTTCTATGGAGGTAAGACTGACCCAATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGAt              >  1:638702/1‑141 (MQ=255)

                                                                                                                          tgCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGGTCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATg             >  2:1028930/1‑141 (MQ=255)

                                                                                                                          tgCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATg             <  1:293808/141‑1 (MQ=255)

                                                                                                                            cGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCa           >  1:740930/1‑141 (MQ=255)

                                                                                                                            cGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCa           >  1:418399/1‑141 (MQ=255)

                                                                                                                              tttCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAAt         <  1:708009/141‑1 (MQ=255)

                                                                                                                              tttCTATGACGGTAAGACTTACCCGATGGAGAAACCTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAAt         <  1:745372/141‑1 (MQ=255)

                                                                                                                                 cTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTgg      >  2:794616/1‑141 (MQ=255)

                                                                                                                                  tATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGt     >  2:955082/1‑141 (MQ=255)

                                                                                                                                     ggCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTgca  >  1:308268/1‑141 (MQ=255)

TATCGGTGTTGGTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTGCA  >  USA300TCH1516_ALE/19130‑19403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A