Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F169 I0 R1	75	34.0	794130	95.7%	759982	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	607,884	G→A	100%	R209* (CGA→TGA)	dck ←	Deoxyadenosine/deoxycytidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	607,884	0	G	A	100.0%	72.5 / NA	26	R209* (CGA→TGA)	dck	Deoxyadenosine/deoxycytidine kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (10/16); total (10/16)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TATATGTGAATTAATGTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCGAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTAAATAGCTTTTTCCAGT  >  USA300TCH1516_ALE/607747‑608021

tatttttgAATTAATGTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGc                                                                                                                                        <  1:29333/135‑1 (MQ=255)

               gTATTCGATAGAATTGATAAAATAATCGAGAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGGCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCAt                                                                                                                         <  2:361419/141‑1 (MQ=255)

                   tCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGa                                                                                                                     <  2:274340/141‑1 (MQ=255)

                                      aatCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCt                                                                                                  <  2:309897/141‑1 (MQ=255)

                                       atCGATAATATCTATGGAATATTCATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCtt                                                                                                 <  2:38425/141‑1 (MQ=255)

                                       atCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCtt                                                                                                 <  1:363898/141‑1 (MQ=255)

                                                  cTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCat                                                                                      >  2:219716/1‑141 (MQ=255)

                                                         atatACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGCTCTAATGATTCGGGGTCCTTATGGATATCCTATTCttt                                                                               >  1:99938/1‑141 (MQ=255)

                                                         atatACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCAtt                                                                               >  1:315719/1‑141 (MQ=255)

                                                              cATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATTGATATCCTATTCATTAatat                                                                          >  2:348728/1‑141 (MQ=255)

                                                                      ataAACATATACGCTATGTCTTTAGTCTTAGATCGTGTATCCACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGt                                                                  <  1:247599/141‑1 (MQ=255)

                                                                         aaCATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACa                                                               >  1:351154/1‑141 (MQ=255)

                                                                                 cGCTATGTCTTTAGTCTTTTATCGTTTATCTACTTGTCGATATGTTTGAATAATGCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGCATATCATATTCATTAATATTGCTACGTACAACTGGACa                                                       <  1:220850/141‑1 (MQ=255)

                                                                                  gCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACAt                                                      >  2:189751/1‑141 (MQ=255)

                                                                                   cTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACTACTGGACATg                                                     <  1:286912/141‑1 (MQ=255)

                                                                                                    tATCGTGTATCTACTTGTAGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGTACATGCATTAAAGCTATTAATc                                    <  2:289143/141‑1 (MQ=255)

                                                                                                     aTCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATcc                                   >  1:123226/1‑141 (MQ=255)

                                                                                                               tACTTGTCGTTATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATa                         <  1:116592/141‑1 (MQ=255)

                                                                                                                       gATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTaa                 <  2:227029/141‑1 (MQ=255)

                                                                                                                       gATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTaa                 <  2:226980/141‑1 (MQ=255)

                                                                                                                          atGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTAAATa              >  1:351024/1‑141 (MQ=255)

                                                                                                                           tggctGAATAATTCAAGCAATTTTGTTTATCAGAGGATGTAAAGATTCGGGGTCCTCATGCATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTAAATAg             <  1:348728/137‑1 (MQ=255)

                                                                                                                           tGTTGGAATAATTCAAGTAATTTTGTTTATCATATGATTTAAAGATTCGGCGTCCTTGTGGATATCATATTCATTAATATTGTTACGTTCAACTGGACATGCATTAAAACTATTAATCAAATCGTCATAGCGTTTAAATAg             <  2:9021/141‑1 (MQ=255)

                                                                                                                              ttGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTAAATAGCtt          >  1:331066/1‑141 (MQ=255)

                                                                                                                                 aataatTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGTATATCATATTCATTAATATTGATACGTACAACTGGCCATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTAAACAGCttttt       <  2:291667/141‑1 (MQ=255)

                                                                                                                                      ttCAAGCAATTTTGTTTATAATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGAGGTTAAACAGCTTATTCTAga  >  2:284854/1‑140 (MQ=255)

TATATGTGAATTAATGTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCGAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTAAATAGCTTTTTCCAGT  >  USA300TCH1516_ALE/607747‑608021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A