Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F17 I0 R1 43 55.2 1410130 81.3% 1146435 215.5

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑C‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAA  >  NC_000913/4296193‑4296622
                                                                                                                                                                                              |                                                                                                                                                                                                                                                   
tggtcttagtAGGCCTACGCCAGGCGGCTCAATAGCCTGGTTTAGTGTGATTTTGGAGGTCGGATAAGGGGTTTATGCCGCGTACGACATCAACGGCTGATGCGACGCTTAACGCGGCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACC                                                                                                                                                                                       <  M01186:129:000000000‑A5M8V:1:1110:17789:13532/241‑1 (MQ=1)
ctgtcatatcaggcatacgccaggcctcgacagagtCTGATTTAGCGTGATTTTTGAGGTCGGTAAAGGCTGTTATTCCGTAACCGACATCCACGTCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCATATCTTAACC                                                                                                                                                                                       <  M01186:129:000000000‑A5M8V:1:1101:17559:24484/215‑1 (MQ=0)
 ggtcttactAGGCCGACGCCAGAAAGCACAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACC                                                                                                                                                                                       <  M01186:129:000000000‑A5M8V:1:2103:14308:7033/241‑1 (MQ=2)
 CGTCTTATCAGGCCTTCGCCAGCAAGCGCAATTGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCG                                                                                                                                                                                      <  M01186:129:000000000‑A5M8V:1:1104:12327:23651/251‑1 (MQ=3)
   TCTTATCAGGCCTACGCCAGACAGCGCAATATCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCTTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGT                                                                                                                                                                                     <  M01186:129:000000000‑A5M8V:1:1102:5806:16182/250‑1 (MQ=5)
                ACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCACTGCCTGAGGCGACGCTTGCCGCGCCTCATCAGGCCGATCTTAACCGTTGGTTAATTTTTTA                                                                                                                                                                       >  M01186:129:000000000‑A5M8V:1:2109:6863:11021/1‑251 (MQ=22)
                             CAATAGCCTTATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGGATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACC                                                                                                                                                          <  M01186:129:000000000‑A5M8V:1:2106:21714:23976/251‑1 (MQ=53)
                                  GCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAACCTCTTCcaacccggat                                                                                                                                                     >  M01186:129:000000000‑A5M8V:1:1107:13227:7757/1‑241 (MQ=38)
                                  GCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTTTTCACACCCGGTA                                                                                                                                                     >  M01186:129:000000000‑A5M8V:1:1101:8623:10292/1‑251 (MQ=54)
                                                 ATTTTGTAGGTTGGATAAGGCTTTTATGCCGTCTCCAAAAGCAAAGCCTGAAGCGACGCTTAATGCGTCTTATCCAGCCTTCGCCAGGCAGCTCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCTACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATA                                                                                                                                      <  M01186:129:000000000‑A5M8V:1:1102:7109:5625/251‑1 (MQ=60)
                                                  TTTTGTAGGTTGGAAAAGGCGTCTAGGCCGCATCCCGCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAC                                                                                                                                     <  M01186:129:000000000‑A5M8V:1:2111:11646:7984/251‑1 (MQ=60)
                                                  TTTTCAAGGTCGGATAAGGCGTTTAGTCCGCAGCCGAAACCAACGCCTGATGCGACGTGTAACGCGTATTATCAGGCCTACGCCCGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGCCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAC                                                                                                                                     <  M01186:129:000000000‑A5M8V:1:1109:18815:12284/251‑1 (MQ=60)
                                                     tgtagggcggatcaggcgtttagtcccacaccgacatcatctcctggtgccgCGCTTAACGCGCCTTATCAGTCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCTTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC                                                                                                                                  <  M01186:129:000000000‑A5M8V:1:2107:12599:24816/199‑1 (MQ=60)
                                                            tggacaaggcctttatcgCGCATCCGACAGCACCGTCTGATGCGACGCTTAACGCGTCTTATCAGGCCGAAGCCAGACAGCGCAATAGCCTGATGTAGCGGGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTT                                                                                                                           <  M01186:129:000000000‑A5M8V:1:1112:23205:4625/233‑1 (MQ=60)
                                                             GGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTAATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCCACCCCGGCATCTAACCCTTCCATACAGCTCTGGTTA                                                                                                                          >  M01186:129:000000000‑A5M8V:1:2114:19276:9860/1‑251 (MQ=50)
                                                              GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCA                                                                                                                         >  M01186:129:000000000‑A5M8V:1:2106:25346:13092/1‑251 (MQ=60)
                                                              GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAACGTTGGTTAATTTTTCAAACTCTTAACACCCGGTATCCAACCCgccccttttacacaactccg                                                                                                                         >  M01186:129:000000000‑A5M8V:1:2108:16689:3306/1‑231 (MQ=50)
                                                                 AAGGGTTTTATGCCGCCTCCGACATCAACGCCTGATGCGACGCTTAACGGGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACC                                                                                                                      <  M01186:129:000000000‑A5M8V:1:2101:21315:5640/251‑1 (MQ=60)
                                                                   GGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGCCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTAGCAAACCCGTCCATACAGCTCAGGTTCAACCAA                                                                                                                    >  M01186:129:000000000‑A5M8V:1:2111:13012:10138/1‑251 (MQ=60)
                                                                       TTTAAGCCGAATCCGGCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCATCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGC                                                                                                                 <  M01186:129:000000000‑A5M8V:1:2104:22799:9507/250‑1 (MQ=60)
                                                                               GCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAACGCCTTT                                                                                                        >  M01186:129:000000000‑A5M8V:1:1109:17233:14869/1‑251 (MQ=60)
                                                                                CATCCGAAAGCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTT                                                                                                       <  M01186:129:000000000‑A5M8V:1:1108:27547:19734/251‑1 (MQ=60)
                                                                                   CCGAAATCAACCCCTGATGCGACGCTTAACGCGTCTTATCGGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCTTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCT                                                                                                    <  M01186:129:000000000‑A5M8V:1:2112:20845:7432/251‑1 (MQ=60)
                                                                                        ATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTC                                                                                               <  M01186:129:000000000‑A5M8V:1:1106:13998:14746/251‑1 (MQ=60)
                                                                                             CGCCTGATGGGACGCTTGCCGCGTCTTATCAGGGCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGC‑‑CGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTC                                                                                          <  M01186:129:000000000‑A5M8V:1:2102:14049:11109/251‑1 (MQ=60)
                                                                                                      atgggtataagggacagTATCAGGTCTACGCCAGAACGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGGCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAAC                                                                                  <  M01186:129:000000000‑A5M8V:1:1111:12154:12228/233‑1 (MQ=60)
                                                                                                      CGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAAC                                                                                  <  M01186:129:000000000‑A5M8V:1:1110:22084:8398/250‑1 (MQ=60)
                                                                                                              TACGCGTCTTATCAGGCCTACGCCAGACATCGCAATAGCCTGATTTAGCGTGAGTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGGCATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCAC                                                                         <  M01186:129:000000000‑A5M8V:1:2104:12668:20053/251‑1 (MQ=60)
                                                                                                                  CGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTT                                                                     >  M01186:129:000000000‑A5M8V:1:1112:18053:6567/1‑251 (MQ=60)
                                                                                                                      TTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACATCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTATTCTTCTCCATAAAACCTTCTTCACCGTTTAAA                                                                 >  M01186:129:000000000‑A5M8V:1:1103:25768:20764/1‑251 (MQ=60)
                                                                                                                         TCAGGCTCACGCCAGACAGCGCAATAGCCGGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGTCATCAATGCCTGATGCGACTCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAC                                                              <  M01186:129:000000000‑A5M8V:1:1104:21905:24419/251‑1 (MQ=60)
                                                                                                                          tgtggtaaaagagacagGCGCAATGGCCTGATTGAGCGTGATTTGGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCGTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAC                                                              <  M01186:129:000000000‑A5M8V:1:1112:21899:22519/233‑1 (MQ=60)
                                                                                                                            GGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCACCCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGGTTAAGAC                                                               >  M01186:129:000000000‑A5M8V:1:2102:15068:25060/1‑247 (MQ=60)
                                                                                                                            GGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCATTAAAACCTTCTTCACCGTTTAAGAAaata                                                           >  M01186:129:000000000‑A5M8V:1:1105:11158:18823/1‑247 (MQ=60)
                                                                                                                            GGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTA                                                                                                                                                     >  M01186:129:000000000‑A5M8V:1:2105:16117:14415/1‑161 (MQ=60)
                                                                                                                                 ACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGCCATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCCAACCCTTCCATACAGCTCAGGTTCAACCATTTCAGCGCCCTTTGCTTTTTCTTCTCCATAAAACCTTATTCACCGTTTAcaaacctccactt                                                      >  M01186:129:000000000‑A5M8V:1:1111:15041:21678/1‑238 (MQ=60)
                                                                                                                                 ACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAG                                                                                                                                   >  M01186:129:000000000‑A5M8V:1:1109:17629:5729/1‑174 (MQ=60)
                                                                                                                                 ACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACC                                                                                                                                                          >  M01186:129:000000000‑A5M8V:1:2103:16108:5732/1‑151 (MQ=60)
                                                                                                                                    CCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGTTAAAACCTTCTTCACCCTTTCAAAACATCATTCCCG                                                    >  M01186:129:000000000‑A5M8V:1:1105:12064:9576/1‑250 (MQ=60)
                                                                                                                                           GCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCATTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTA                                             >  M01186:129:000000000‑A5M8V:1:1104:6001:15418/1‑250 (MQ=60)
                                                                                                                                            cgcaatggcgtttgTTAGCGTGATTTTGTAGGTAGGATAAGGCGTTTC‑‑CGACGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCTTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAATCCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTCTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCGCGCCCAGTAC                                            <  M01186:129:000000000‑A5M8V:1:2101:4171:10817/236‑1 (MQ=60)
                                                                                                                                                attgccgaatttagcggtattggtgagggCGGATAAGGCGTTTACCC‑‑CGCATCCGACTTCAATGCCTGATTCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGA                                       <  M01186:129:000000000‑A5M8V:1:1105:16201:5952/222‑1 (MQ=60)
                                                                                                                                                atacgCTGATTTAACGTGATTTTGAAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCCACGCTTGCCGCGTCTTAGCAGGCCTATCGTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGA                                       <  M01186:129:000000000‑A5M8V:1:1109:22713:4520/246‑1 (MQ=60)
                                                                                                                                                TTAGCCTGATTTAGCGTGAGTTTGTAGTGCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTACCCGTTGGTTAATTTTTCAATCTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGA                                       <  M01186:129:000000000‑A5M8V:1:1106:8369:13833/251‑1 (MQ=60)
                                                                                                                                                ATAGCCTGATTTAGCGTGGTTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCGATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAATCTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGA                                       <  M01186:129:000000000‑A5M8V:1:2112:27559:18980/251‑1 (MQ=60)
                                                                                                                                                 TAGCCTTATTTAGGCTGATTTTGTAGGTCGGATAAGGCATTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCAATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAA                                      <  M01186:129:000000000‑A5M8V:1:1103:24822:6137/251‑1 (MQ=60)
                                                                                                                                                   GCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATA                                    >  M01186:129:000000000‑A5M8V:1:1106:18250:16649/1‑251 (MQ=60)
                                                                                                                                                                     TTGTGGGGCGGATAAGGCCTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCCGCGCTTGCCGCGTCTTACCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGC                  <  M01186:129:000000000‑A5M8V:1:2110:14565:9075/251‑1 (MQ=60)
                                                                                                                                                                     TTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTG                   >  M01186:129:000000000‑A5M8V:1:2105:13707:4565/1‑250 (MQ=60)
                                                                                                                                                                           GGCGGATAAGGCGTTTACGC‑‑CGCATCCGAACTCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACGCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGC             <  M01186:129:000000000‑A5M8V:1:1109:28723:13561/250‑1 (MQ=60)
                                                                                                                                                                                    GGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTTAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGTTAAAACCTACTTCACCGTTTAAGAAAATCATTCCCGCCCCGTCCTCGGTATACCCGGGTCGGGAAATTGCcacgttccgtttgca   >  M01186:129:000000000‑A5M8V:1:1104:17755:2056/1‑236 (MQ=60)
                                                                                                                                                                                     GCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAA  >  M01186:129:000000000‑A5M8V:1:2109:28937:17329/1‑251 (MQ=60)
                                                                                                                                                                                              |                                                                                                                                                                                                                                                   
GCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑C‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAA  >  NC_000913/4296193‑4296622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑