Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I0 R1 51 55.9 1320120 86.3% 1139263 218.0

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑A‑‑C‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAA  >  NC_000913/4296193‑4296622
                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     
cggtcttgtcaggccacCGCCAGGCAGGGCAATAGCCTGATTTAGCGTGATTTGGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACC                                                                                                                                                                                       <  M01186:129:000000000‑A5M8V:1:1107:16371:7386/234‑1 (MQ=2)
 CGTCTTTGCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAGGCCGGATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACC                                                                                                                                                                                       <  M01186:129:000000000‑A5M8V:1:2101:10806:20834/250‑1 (MQ=2)
 CGTCTTATCAGGCCTGCGCCAGATAGCGCAATAGGCTGATTTAGCGTGATTTTGTGGGTAGGAAAAGGCGTTTATGCCGAAGCCGACAGCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACC                                                                                                                                                                                       <  M01186:129:000000000‑A5M8V:1:1113:5360:15773/250‑1 (MQ=1)
    TTTATCAGGGCTACGCCAGGCAGCGCAATCGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTG                                                                                                                                                                                   <  M01186:129:000000000‑A5M8V:1:1101:19729:25974/251‑1 (MQ=8)
      TATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATACGCAGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGT                                                                                                                                                                                 >  M01186:129:000000000‑A5M8V:1:2103:7923:21578/1‑251 (MQ=15)
                ACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGTTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCGGATTCGACGTTTGCCGCTTCTTACCTGGCCTATCTTACCCGTTGGTGAATTTTTCA                                                                                                                                                                       >  M01186:129:000000000‑A5M8V:1:1114:20580:13175/1‑251 (MQ=12)
                ACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGAAGCTTGCCGCGTCTTATCAGGCCTACCTTAACCGTTGGTTAATTTTTCA                                                                                                                                                                       >  M01186:129:000000000‑A5M8V:1:2106:20586:9620/1‑251 (MQ=22)
                        CAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTT                                                                                                                                                                >  M01186:129:000000000‑A5M8V:1:2103:17677:24220/1‑250 (MQ=45)
                             CAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAACCTCTTCACAC                                                                                                                                                           >  M01186:129:000000000‑A5M8V:1:2107:9588:7884/1‑250 (MQ=44)
                                   CCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGCACTCACCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTA                                                                                                                                                     <  M01186:129:000000000‑A5M8V:1:2114:14551:20459/250‑1 (MQ=60)
                                      GATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGGGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAAGGCCTGATGCGACGCTTGCCTCGACTTTTAAGTCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCGCACCCCGTTTCAA                                                                                                                                                 >  M01186:129:000000000‑A5M8V:1:1106:20430:16710/1‑251 (MQ=22)
                                        tttactgtgattttttagggctgatagtggcttttgGCCGCAGCCGACATCAACCCCTGAAGCGACGTTTATCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑TCATCCTACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAC                                                                                                                                               <  M01186:129:000000000‑A5M8V:1:1108:2751:13545/215‑1 (MQ=60)
                                         TTAGCGTTATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACC                                                                                                                                              <  M01186:129:000000000‑A5M8V:1:1101:20360:13311/251‑1 (MQ=60)
                                         TTAGCGGGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACC                                                                                                                                              <  M01186:129:000000000‑A5M8V:1:1108:27368:20456/251‑1 (MQ=60)
                                                GATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCAA                                                                                                                                       >  M01186:129:000000000‑A5M8V:1:1109:8277:6862/1‑251 (MQ=60)
                                                 ATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACC                                                                                                                                                                                       <  M01186:129:000000000‑A5M8V:1:1105:15595:17928/202‑1 (MQ=3)
                                                  tgtgGTAGGTCGGGTAAGGGGTTTAGTCCGCATCCGACATCAACGCCTGGTGCGAGGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTGAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAC                                                                                                                                     <  M01186:129:000000000‑A5M8V:1:2111:21047:5358/247‑1 (MQ=60)
                                                  TTTTGTAGTGCGGATAAGTCGGTTATGCCCGATCCGAAATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAC                                                                                                                                     <  M01186:129:000000000‑A5M8V:1:2111:19454:16859/251‑1 (MQ=60)
                                                             GGATAAGGCGTTTAGGCCGCATCCGACATAAACGCCTGATGCGACGCTTAACGTGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTT                                                                                                                           <  M01186:129:000000000‑A5M8V:1:2109:16443:5423/250‑1 (MQ=60)
                                                             GGAAAAGGGGTTTATGCCGCAACCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTT                                                                                                                           <  M01186:129:000000000‑A5M8V:1:2108:15546:8967/250‑1 (MQ=60)
                                                              GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTACTTTTTCAACATCTTCACACCCGGTATCAAACCCTTCCATACGACTCAGGTTAA                                                                                                                         >  M01186:129:000000000‑A5M8V:1:2105:12719:20591/1‑251 (MQ=60)
                                                                 AAGGCGTTGTTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTAGCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACC                                                                                                                      <  M01186:129:000000000‑A5M8V:1:2108:27401:15550/251‑1 (MQ=60)
                                                                  agggcTTTATGCCGCCTTCGACATCAACGCCTGATGCGACACTTAACTCCTCTTATCAGGCATACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGGCGGATAA‑GGCTTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACC                                                                                                                      <  M01186:129:000000000‑A5M8V:1:1113:10994:8377/245‑1 (MQ=60)
                                                                   GGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAG                                                                                                                    >  M01186:129:000000000‑A5M8V:1:1101:18188:22029/1‑251 (MQ=60)
                                                                        TTAGGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAG                                                                                                               <  M01186:129:000000000‑A5M8V:1:2104:14120:23839/251‑1 (MQ=60)
                                                                                  TCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTAATGCGTCGCATGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCTAACCC                                                                                                                                             >  M01186:129:000000000‑A5M8V:1:1106:21858:21119/1‑211 (MQ=60)
                                                                                   CCGAAATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTAAGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCT                                                                                                    <  M01186:129:000000000‑A5M8V:1:2108:27088:12902/251‑1 (MQ=60)
                                                                                        ATCAACGCCTGATGCGGCGCTTACCGCGTCTTATCAGGCCTAGGCCAGACAGCGCAATAGCCGGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑A‑‑CGAC‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTTTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTC                                                                                               <  M01186:129:000000000‑A5M8V:1:1109:18563:17839/251‑1 (MQ=60)
                                                                                                        GCGCTTAACGTGTCTTAACCGGCCTACCCCAGGCAGCTCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCT                                                                                <  M01186:129:000000000‑A5M8V:1:2114:26189:8562/250‑1 (MQ=60)
                                                                                                          GCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATCAAACCTT                                                                               >  M01186:129:000000000‑A5M8V:1:2104:19543:11290/1‑249 (MQ=60)
                                                                                                                      tttcCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAG                                                                 <  M01186:129:000000000‑A5M8V:1:2114:19106:8230/247‑1 (MQ=60)
                                                                                                                      TTAACAGGCCTACGCCAGACCGCGCAATATCCTGATTTAGCGGGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCATATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAG                                                                 <  M01186:129:000000000‑A5M8V:1:2103:21580:10085/251‑1 (MQ=60)
                                                                                                                        TTCAGGCCTACGCCAGACAGCGGAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAA                                                               <  M01186:129:000000000‑A5M8V:1:2101:18543:8323/251‑1 (MQ=60)
                                                                                                                                 ACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATCCAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGCTAAAACCTCCTTCACCGTTTAAGCACATCATTC                                                       >  M01186:129:000000000‑A5M8V:1:1107:20312:25382/1‑250 (MQ=60)
                                                                                                                                 ACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGCTAAAACCTTCTTCACCGTTTAAGAACATCATTC                                                       >  M01186:129:000000000‑A5M8V:1:1103:13393:10964/1‑250 (MQ=60)
                                                                                                                                 ACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTCCCATACAGCTCAGGTTCAACCAGTGCAGCCCCTTTTGCTTTTTCTACTCGATAAAACCTTCTacacctcttcagaaaatacttc                                                       >  M01186:129:000000000‑A5M8V:1:2106:13457:22810/1‑228 (MQ=60)
                                                                                                                                 ACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGGAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCAGCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC                                                                                                                                  <  M01186:129:000000000‑A5M8V:1:1111:24553:20251/175‑1 (MQ=60)
                                                                                                                                         CAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAC                                                                                                                                     >  M01186:129:000000000‑A5M8V:1:2105:22587:22151/1‑164 (MQ=60)
                                                                                                                                            CGCAATAGCCGGGTTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTAC                                            <  M01186:129:000000000‑A5M8V:1:1110:25017:13634/250‑1 (MQ=60)
                                                                                                                                                ATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGA                                       <  M01186:129:000000000‑A5M8V:1:1107:10160:8133/251‑1 (MQ=60)
                                                                                                                                                  TGCCTGATTTAGCGTGATTTTGTAGTTCGGATAA‑GGCTTTTGCG‑‑C‑‑T‑‑GCTTCCGACTTCAATGCCTGATGCGACGCTTGCCGGGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCAGACAGCTCCGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGA                                       <  M01186:129:000000000‑A5M8V:1:2103:5337:14497/249‑1 (MQ=60)
                                                                                                                                                     CTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAAC                                  <  M01186:129:000000000‑A5M8V:1:2106:14978:19267/251‑1 (MQ=60)
                                                                                                                                                     CTGATTTAGCAGGATTTTGTAGGCCGGATAAGGGCGTTT‑‑C‑‑C‑‑CCTG‑ATCCGACATCAATGCCTGATTCCACGCTTGCCGCGTCTTATCAAGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCGTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAAC                                  <  M01186:129:000000000‑A5M8V:1:2113:15278:6485/251‑1 (MQ=60)
                                                                                                                                                       GATTTAGCGTGATTTTGTAGGTCGGCTAA‑GGCGTTT‑‑ACGC‑‑C‑‑GAATCCGACATCAATGCCTGAGGCGACGCTGGCCGCGTCTTGTCAGGCCTACCTTAACAGTGGGTTAATTTTTCAACCTCTTCACACCCGGTATCAAACCCTTCCATACTGTTCCGGTTAACCCATTTTAGCGCCTATGGCTTGTTCTTATCGCTAAAACCTTTTTCCCCGTTTCACAACATCATTCCCTCCCAGTACACGTAATAACCG                                >  M01186:129:000000000‑A5M8V:1:1113:9540:16691/1‑251 (MQ=57)
                                                                                                                                                                     TTGTAGGTCGGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGA                  >  M01186:129:000000000‑A5M8V:1:2108:27832:19859/1‑251 (MQ=60)
                                                                                                                                                                     TTGTAGGTCGAATAA‑GGCTTTT‑‑ATGC‑‑C‑‑GAATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGCTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGC                  <  M01186:129:000000000‑A5M8V:1:2106:21898:16502/251‑1 (MQ=60)
                                                                                                                                                                              GGATAA‑GGCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGACTAACCGGTTCGGGAAATTGCAGAGCTG           >  M01186:129:000000000‑A5M8V:1:1105:3688:18413/1‑249 (MQ=60)
                                                                                                                                                                                      GCGTTT‑‑ACGC‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAA  >  M01186:129:000000000‑A5M8V:1:1102:11137:16624/1‑251 (MQ=60)
                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     
GCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAA‑GGCGTTT‑‑A‑‑C‑‑C‑‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAA  >  NC_000913/4296193‑4296622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑