Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F17 I0 R1 109 78.2 1797446 84.3% 1515246 230.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5430CA100.0% 393.7 / ‑6.7 111P1100Q (CCG→CAG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (53/56);  total (54/57)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 111 total aligned reads displayed.

GAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGAT‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑AGAAAGTTGACCTGAGTACCTTCAGCGATG‑AAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAA  >  NC_000913/4184307‑4184777
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            
gAAACACGAGTTCGAGAAGAAACTCGAAGGGAAACGGCGAAAAAGCACCCAGGGCGACGAT‑CGGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGc                                                                                                                                                                                                                                            <  1:825990/239‑1 (MQ=255)
gAAACACGAGTTCGAGAAGAAACTCGAAGCGAAGCGCCGCAAAATCACCCAGGGCGACGAG‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGc                                                                                                                                                                                                                                            <  1:241848/239‑1 (MQ=255)
       gAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGAT‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCCGCCTGGGGACAAAATGGCAGGTCGTCACGGTAACAAGGGGGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTCAGATGAAAACGGAACGCCGGCAGACATCGTACGAAACAAGCTggggga                                                                                                                                                                                                                                     >  2:862873/1‑236 (MQ=255)
       gAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGAT‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGGGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATTAAAACGGTACGCCGGTCAACATCGTACTGAACCAGCTGGGCGt                                                                                                                                                                                                                                     >  1:16975/1‑239 (MQ=255)
         gTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGAT‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTa                                                                                                                                                                                                                                    <  1:645520/238‑1 (MQ=255)
         gTTCGAGAAGAAACGCGAAGCGAACAGCCGCAAAAGCACCCAGGGCTACGAT‑CGGGCACCGTGCGTGCTGAAGAGTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGGGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTa                                                                                                                                                                                                                                    <  1:381338/238‑1 (MQ=255)
            ggagaAGAAACCCGAAGGGAAACGCCCTAAACACCCCCAGGGAGACGGT‑CTGGCACCGTGCGGGCTGGAGATTTGTAATGTAGATCTGGCGGGTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGt                                                                                                                                                                                                                                <  2:229162/238‑1 (MQ=255)
            cGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGCGACGAT‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGt                                                                                                                                                                                                                                <  1:531186/239‑1 (MQ=255)
            cGAAAAGAAACGCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGAG‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTTTCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGt                                                                                                                                                                                                                                <  1:867842/239‑1 (MQ=255)
              ggaagaaACCCGAAGCGAAACCACGCAAAAATCCCCAGGGACAGGCT‑CTGGCCCCGGGCGTTCTGAAGATTGTTAATGTATACCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGAGCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtct                                                                                                                                                                                                                              <  2:348850/238‑1 (MQ=255)
              gaaagtaACTCGGAACGAAACGCCGGAAAATCCCCCCGGGCGGCGAT‑CTGGCACCGGGCTGGCTGAAGATTGGTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGGCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtct                                                                                                                                                                                                                              <  1:379729/237‑1 (MQ=255)
                 agaaACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGCGACGAT‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGt                                                                                                                                                                                                                           <  1:82027/239‑1 (MQ=255)
                 agaaACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGAT‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGGGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACCCCGGTCAAAATCGAACTTAACCAGCTGGGGGAACCGACTGGt                                                                                                                                                                                                                           >  2:82027/1‑239 (MQ=255)
                   aaaCTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGAT‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAt                                                                                                                                                                                                                         >  1:516848/1‑239 (MQ=255)
                   aaaCTCGAAGCGAAACGCCGAAAAATCACCCAGGGCGACGAT‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATAGCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAt                                                                                                                                                                                                                         <  1:313952/239‑1 (MQ=255)
                      ccagcaacaaaaCCCCGGAAAATCCCCCAGGGCCGCGATGTTGGCA‑CGGGCGTGCTGAAGAGTGTTAAGGTATATCTGGCGGTTAAACTACGTAGCCAGCCGTGTGACAAGAGGGCAGGTCGTCACGGTAACAAGGGTGTAAATTCTAAGATCACCCCTATCGAAGATATTCCTTACGATGAAAACGGTACGCCGGTAGACATCTTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa                                                                                                                                                                                                                      <  2:31129/230‑1 (MQ=255)
                        cGAAGCGAAACGCAGCAAACTCCCCCAGGGCGACGAT‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACa                                                                                                                                                                                                                    <  1:728719/239‑1 (MQ=255)
                              gAAACGCCGCAAAATCCCCCAGGCCGACGAT‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGt                                                                                                                                                                                                               <  1:852790/238‑1 (MQ=255)
                              gAAACGCCGCAAAATCACCCAGGGCGACGAT‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGt                                                                                                                                                                                                               <  1:869899/238‑1 (MQ=255)
                               ggcagccgcAAAATCACCCAGGGCGACGAT‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGt                                                                                                                                                                                                               <  1:419059/233‑1 (MQ=255)
                               aaaCGCCGCAAAATCACCCAGGGCGACGAT‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACa                                                                                                                                                                                                                    <  2:143918/232‑1 (MQ=255)
                               aaaCGCCGCAAAATCACCCAGGGCGACGAT‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACa                                                                                                                                                                                                                    >  1:143918/1‑232 (MQ=255)
                                     cgcAAAATCACCCAGGGCGACGAT‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATAACTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACCTCGTACTGGACCCGCTGGGCGTACCGTCCCGTATGAACATCGGTCAGATcc                                                                                                                                                                                                        >  1:41094/1‑238 (MQ=255)
                                         aacacccccAGGGGGACGTC‑TTGGCACCGGGGGTGCTGAAGATTGTTAAGGTATACCTGGCGGTTAAACGCCGTACCCGGTCTGGTGACACGTAGGCAGGGCGTCACGGTAACAAGGGTGTAATGTCAAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAGGAACAGCGGTCAGATCCTCgca                                                                                                                                                                                                   <  2:891443/233‑3 (MQ=255)
                                           accccccGGGGCGACTAA‑TCGGCACCGGGCTGTCGTAAGATTGTTAAGGTATAGTGGGCGGTTAAAACACGTAGCCAGCCTGGTGACAAGATGGCAGGTCGGCACGGTAACAAGGGTATAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCGCGAAAc                                                                                                                                                                                                 <  2:831348/235‑1 (MQ=255)
                                           aTCACCCAGGGCGACGAT‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAc                                                                                                                                                                                                 <  2:648950/239‑1 (MQ=255)
                                               cccAGGGCGACGAT‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGGGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCCGGGCGGACCGTCTCGTATAAAAATCGGGCAAATCCCCGacggtc                                                                                                                                                                                               >  2:419059/1‑232 (MQ=255)
                                               cccAGGGAGACGGT‑TTGGCACCGGGGCTTCTGTAGGTTGTTAAGGTATATCGGGGGGGTAAACGGCGTAACCAGCCTGGTAACAAGATGGCAGGTCGTCACTGTAACAAGGGTGTAATTTCGAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAACCCCAc                                                                                                                                                                                             <  2:283449/239‑1 (MQ=255)
                                                    ggCGACGAT‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATAGTACTGAACAAGCTGGGCTTACCGTCTCGTATGAACATCGCTCAGATCCTCGAAACCCACCTggg                                                                                                                                                                                        >  1:768468/1‑239 (MQ=255)
                                                               tGGCACCGGGAGTGCGGAAGATTGTGAAGGTATAGCTGGCGGTTAAACGCCGTAGCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCg                                                                                                                                                                              <  2:671191/239‑1 (MQ=255)
                                                                       ggCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAggaa                                                                                                                                                                       >  1:228635/1‑236 (MQ=255)
                                                                       ggCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTa                                                                                                                                                                       >  1:105716/1‑238 (MQ=255)
                                                                       ggCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGGGGAATTTCTAAGATCAACCCGATCGAAGCTATGCCTTACGATGAAAACGGTACGCCGGTAGCCATCGTACTGAACCAGCTGGGCGTACCGTCCCGAATGAACATCGGTCAGATCCCCGAAACCCACCTGGGTATGGCTGGGAAAGGAAt                                                                                                                                                                      >  2:853740/1‑239 (MQ=255)
                                                                           tGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGAATGGCTGGCAAAGGGAGCGGc                                                                                                                                                                  >  1:323793/1‑239 (MQ=255)
                                                                               gAAGATTTTTAATTTTTATTTGGCGGTTAAACTCCGTGTCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACa                                                                                                                                                              <  2:305144/239‑1 (MQ=255)
                                                                               gAAGATTTTTAAGGTATAGCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGAc                                                                                                                                                               <  1:738683/238‑1 (MQ=255)
                                                                                         aaGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGc                                                                                                                                                    <  1:271565/239‑1 (MQ=255)
                                                                                         aaGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGc                                                                                                                                                    <  1:493032/239‑1 (MQ=255)
                                                                                          aGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGCTCAACGcc                                                                                                                                                   >  1:812229/1‑239 (MQ=255)
                                                                                                 tCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTAAGATCCCCGAAACCAACCTGGGGTTGGCCGGGAAAGGGATTCGCGACAAGATaaaacccactctca                                                                                                                                            >  2:493032/1‑228 (MQ=255)
                                                                                                      cGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGCTATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGCCTCGTATGAACATCGGTAGGATCCTCGAAACCCACCCGGGAATGGCTGCGAAAGGGATCGGCGCAAAAATCCACGCCATGGTGaaaacg                                                                                                                                       >  1:215614/1‑236 (MQ=255)
                                                                                                                   tATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGGATCGGCGCCAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcc                                                                                                                          >  1:77559/1‑238 (MQ=255)
                                                                                                                   tATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGAATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTc                                                                                                                           >  1:829836/1‑238 (MQ=255)
                                                                                                                   tATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGAATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTc                                                                                                                           >  1:820242/1‑238 (MQ=255)
                                                                                                                      ccccccgggTGACAAGTTTGAAGGTCGTAAGGGTAAACAGGGTGAAATTTGAAAGATCAACCCGATCGAATATAGGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGACTCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGa                                                                                                                       <  2:241572/232‑1 (MQ=255)
                                                                                                                          ccTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGc                                                                                                                                                                                                                                            >  1:347646/1‑118 (MQ=255)
                                                                                                                          ccTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCCAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGc                                                                                                                                                                                                                                            <  2:347646/118‑1 (MQ=255)
                                                                                                                             ggTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcg                                                                                                                <  2:516848/239‑1 (MQ=255)
                                                                                                                             ggTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACACAAGCAAGAAGTCGCGAAAcgccc                                                                                                                >  1:294257/1‑235 (MQ=255)
                                                                                                                              gTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCCAGAAGCGCCGAAACTgcgc                                                                                                               >  1:497010/1‑239 (MQ=255)
                                                                                                                              gTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGGATCGGCGAAAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTGCGGAAACAgcgc                                                                                                               >  2:712746/1‑239 (MQ=255)
                                                                                                                                     gATGGCAGGTCGGCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGGGAAAGGAAGCGGCGGCCAGAACAACCCCCTGCGGAAACAACAGCCAAAAATCCCGAAACCGCGAGAATTc                                                                                                         >  2:494736/1‑238 (MQ=255)
                                                                                                                                        ggCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCCCCTGGGAATGGCTGGGAAAGGTATCGGGGGCAAGATCAACGCCATGCGTAAAAAGCAGCAAAAAGACCCGAAAACGCGCGAATTccacc                                                                                                     >  2:21693/1‑235 (MQ=255)
                                                                                                                                        ggCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGGATGGCTCCGAAAAGACTCGGGCACAAAGTCAACCCCATGCTGAAAACGCAGCAAAAAACCCGGAAAATGCCCGaactaaacc                                                                                                     >  2:888151/1‑232 (MQ=255)
                                                                                                                                             gtcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAgcgt                                                                                                >  1:325688/1‑239 (MQ=255)
                                                                                                                                             gtcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAgcg                                                                                                 <  1:19887/238‑1 (MQ=255)
                                                                                                                                              tcgtcCCGGTAACAAGGGTGTAATTTCTAAAATCAACCCGATCGAAGAAATGCCTTACGATGAAAACGGGACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAgc                                                                                                  <  2:228635/236‑1 (MQ=255)
                                                                                                                                                  cACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTa                                                                                           <  1:154338/239‑1 (MQ=255)
                                                                                                                                                             gggTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTa                                                                                                                                                                                                                                    <  1:346497/91‑1 (MQ=255)
                                                                                                                                                             gggTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCATACTGAACCAGCTGGGCGTa                                                                                                                                                                                                                                    >  2:346497/1‑91 (MQ=255)
                                                                                                                                                               gtgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGgc                                                                                 >  1:82453/1‑236 (MQ=255)
                                                                                                                                                               gtgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCACGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGGGCGTACGCTCTGGgcgcg                                                                              >  1:781144/1‑238 (MQ=255)
                                                                                                                                                                 gtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGAATCGGGGCCAAGATCAACCCCATGCTGAACAAGCCACAAGAAATCGCGAAACTGCGCGAATTCATCCAACGAGCGGACGAAATGGGGGGTGa                                                                            >  2:424038/1‑239 (MQ=255)
                                                                                                                                                                  tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTAATCCACCGTGCGTACGATCTGGGCGCTGAc                                                                           >  1:184775/1‑239 (MQ=255)
                                                                                                                                                                   aaTTTCTAAGACTAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGCCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACg                                                                          <  2:603279/239‑1 (MQ=255)
                                                                                                                                                                    aTTTCTAAGATCAACCCGATCGAAGATATGCCTTAAGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGt                                                                         <  1:212419/239‑1 (MQ=255)
                                                                                                                                                                     tttCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTAGGATCTGGGGGCTGACGtt                                                                        >  1:824404/1‑239 (MQ=255)
                                                                                                                                                                          aaGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑a                                                                  <  1:67466/239‑1 (MQ=255)
                                                                                                                                                                                 aCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑AGAAAGtt                                                           <  1:554924/239‑1 (MQ=255)
                                                                                                                                                                                  cccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTAATCCAGCGTGAGGACGAACTGGGGGCTGACGTTGGTC‑AGAACGTTg                                                          >  1:491752/1‑239 (MQ=255)
                                                                                                                                                                                    ccaTCGAAGATATGCCTTACGAGTAAAACGGTACGCCGGTAAACACCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑AGAAAGTTGAc                                                        <  2:464407/237‑1 (MQ=255)
                                                                                                                                                                                    cGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑AGAAAGTTGAc                                                        <  1:424038/239‑1 (MQ=255)
                                                                                                                                                                                     gaactagaatatGCCTTACGGTGAAAACGGTACGCCGGTATACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCCCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑AGAAAGTTGAcc                                                       <  1:560527/231‑1 (MQ=255)
                                                                                                                                                                                       tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGCATCGGCGACACGATCAACGCCATGCTGAACACGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGACCTGGGCGCTCAAGTTGGTC‑AGAAAGTTGaacgg                                                     >  2:417357/1‑235 (MQ=255)
                                                                                                                                                                                        cGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGCCGTTCGTC‑AGAAAGTTGACCTgc                                                    >  1:759291/1‑238 (MQ=255)
                                                                                                                                                                                          aaGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑CGAAAGTTGACCTGAGt                                                  >  1:541104/1‑239 (MQ=255)
                                                                                                                                                                                                tGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGACATCATCCAGCGGGCGGACGAACTGGGCGCTGACGTTCGTC‑GGAAAATTGACCCGAGGAcccta                                            >  2:105261/1‑236 (MQ=255)
                                                                                                                                                                                                 gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGgcgc                                                                               <  2:379144/204‑1 (MQ=255)
                                                                                                                                                                                                 gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCCCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGAATGGCTGCGAAATGGATCGGCGACAAGATCAACGCCATGCTGACACAGAAGCAAGAAGTCGCCAAACTCCGCGAATTCATCCAGAGTGCGTACGATCTGGGGGCTGACGTTCGGC‑AGAAAATTGACCTGATGACATTCa                                           >  2:630978/1‑239 (MQ=255)
                                                                                                                                                                                                    ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑AGAAAGTTGACCTGAGTACCTTCAGCg                                        <  1:417511/239‑1 (MQ=255)
                                                                                                                                                                                                     ttcGATGAAAACGGGACGCCGGTAGACATCGGACGTACCCAGCGTGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑AGAAAGTTGACCTGAGTACCTTCAGCGa                                       <  2:294257/237‑1 (MQ=255)
                                                                                                                                                                                                     tACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGCAGTCGCGAAACTGCGCGAATTCATCCACCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑AGAAAGTTGACCTGAGTACCTTCAGCgc                                       >  1:330875/1‑238 (MQ=255)
                                                                                                                                                                                                     tACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑AGAAAGTTGACCTGCGTACCTTCAGc                                         >  1:441471/1‑237 (MQ=255)
                                                                                                                                                                                                     tACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑ACAAAGTTGACCTGAGAACCTTCAACgg                                       >  2:417511/1‑238 (MQ=255)
                                                                                                                                                                                                     tACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGACGCGAAACTGCGCGAATTCATCCAGCGTGCGTCCGGTCTGGGCCGTGACGGTCGTCGAGAATG‑TGACCTGAGTACCTTCAGCGa                                       >  2:645429/1‑239 (MQ=255)
                                                                                                                                                                                                       cggttaaaaCGGTTCGCCGTTAGACATCGTACTGAACCAGCTGGGCGTTCCGTCTCGTATGAACCTCGGTCAGATCCTCGAAACCCACCTGGGTAAGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑AGAAAGTTGACCTGAGTACCTTCAGCGATg                                     <  2:82453/234‑1 (MQ=255)
                                                                                                                                                                                                         aTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑AGAAAGTTGACCTGAGTACCTTCAGCGATg‑aa                                  <  1:395775/239‑1 (MQ=255)
                                                                                                                                                                                                          tGAAAACGGTCCGCCGGTACACCTCGTACTGAACCAGCTGGGCGTTCCGTTTCGTATGAACAGCGGTCAGTACCTCGAAACCCCCCTTGGTATGGCTTCGAAAGGTATCGGTGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑AGAAAGTTGACCTGAGTACCTTCAGCGATg‑aag                                 <  2:275852/239‑1 (MQ=255)
                                                                                                                                                                                                          tGAAAACGGTACCCCGGTTGACCGCGTACTGAACCAGCTGGGGGTACCGGCTCCGATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCCGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑AGAAAGTTGACCTGAGTACCTTCAGCGATg‑aa                                  <  2:184775/238‑1 (MQ=255)
                                                                                                                                                                                                            aaaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGAATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGGCCTGACGTTCGTC‑CGAAAGTTCACCTGAGGACCTTCAGCGATg‑acgaa                               >  1:831201/1‑239 (MQ=255)
                                                                                                                                                                                                                        cGGGAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑AGAAAGTTGACCTGAGTACCTTCAGCGATG‑AAGAAGTTATGCGTCt                    <  1:8075/238‑1 (MQ=255)
                                                                                                                                                                                                                           tAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCAGCGACAAGATAAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGGGCTGACGTTCGCC‑AGACAGTTGACCTGAGGACCTTCAGCAATG‑AAGAAGATAATCGTCTgggt                >  2:71160/1‑237 (MQ=255)
                                                                                                                                                                                                                                  cGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCCGGGCGCCGACGTTCGTC‑AGAAAGTTGACCTGAGTACCTTCTGCGATG‑AAGAAGTTATGCCTCTGGCTGAAAAc          >  2:668690/1‑238 (MQ=255)
                                                                                                                                                                                                                                     aCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑AGAAAGTTGACCTGAGTACCTTCAGCGATG‑AAGAAGTTATGCGTCTGGCTGAAAACCTg       >  1:415690/1‑238 (MQ=255)
                                                                                                                                                                                                                                      cTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑AGAAAGTTGACCTGAGTACCTTCAGCGATG‑AAGAAGTTATGCGTCTGGCTGAAAACCTgcg     <  1:862873/239‑1 (MQ=255)
                                                                                                                                                                                                                                        gAACGCGCTGGGTGTTCCCTTCCGTATGAACGTCGGTCAGATCCAGCAAAACCACCTGGGTATGGCTGAGAAAGGTTTCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGAGCTGGGCGCTGACGTTCGTC‑AGAAAGTTGACCTGAGTACCTTCAGCGATG‑AAGAAGTTATGCGTCTGGCTGAAAACCTGCGCa   <  2:105716/239‑1 (MQ=255)
                                                                                                                                                                                                                                        gAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑AGAAAGTTGACCTGAGTACCTTCAGCGATG‑AAGAAGTTATGCGTCTGGCTGAAAACCTGCGCa   <  1:21693/239‑1 (MQ=255)
                                                                                                                                                                                                                                        gAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑AGAAAGTTGACCTGAGTACCTTCAGCGATG‑AAGAAGTTATGCGTCTGGCTGAAAACCTGCGCa   <  1:736382/239‑1 (MQ=255)
                                                                                                                                                                                                                                         aaCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑AGAAAGTTGACCTGAGTACCTTCAGCGATG‑AAGAAGTTATGCGTCTGGCTGAAAACATGCGCaa  >  1:446945/1‑239 (MQ=255)
                                                                                                                                                                                                                                         aaCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCAGGCTGAAACAGCAGCAAGAAGTCGCGAAAATGCGTGAATTCATCCAGCGTGCGTAGGATCTGGGCGCGGAGTTTCCTC‑AGCAAATGTACCTAGGTACCTT‑AGTCCTGTAAAAAGTTAAGCGTCGGGCTGAAAACCTGCGaaa  >  2:23626/1‑239 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            
GAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGAT‑CTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC‑AGAAAGTTGACCTGAGTACCTTCAGCGATG‑AAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAA  >  NC_000913/4184307‑4184777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCA  >  NC_000913/4184303‑4184787
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    
AACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGGGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATTAAAACGGTACGCCGGTCAACATCGTACTGAACCAGCTGGGCGTA                                                                                                                                                                                                                                            >  M01186:129:000000000‑A5M8V:1:1101:21445:15476/1‑251 (MQ=60)
   TGAAACACGAGTTCGAGAAGAAACTCGAAGGGAAACGGCGAAAAAGCACCCAGGGCGACGATCGGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG                                                                                                                                                                                                                                         <  M01186:129:000000000‑A5M8V:1:2112:17566:20665/251‑1 (MQ=60)
   TGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAGCGCCGCAAAATCACCCAGGGCGACGAGCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG                                                                                                                                                                                                                                         <  M01186:129:000000000‑A5M8V:1:1108:4604:18127/251‑1 (MQ=60)
            AGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATG                                                                                                                                                                                                                                >  M01186:129:000000000‑A5M8V:1:2103:27721:14960/1‑251 (MQ=60)
            AGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAT                                                                                                                                                                                                                                 <  M01186:129:000000000‑A5M8V:1:2107:6023:13991/250‑1 (MQ=60)
            AGTTCGAGAAGAAACGCGAAGCGAACAGCCGCAAAAGCACCCAGGGCTACGATCGGGCACCGTGCGTGCTGAAGAGTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGGGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAT                                                                                                                                                                                                                                 <  M01186:129:000000000‑A5M8V:1:1113:18223:1723/250‑1 (MQ=60)
               TCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAAC                                                                                                                                                                                                                             <  M01186:129:000000000‑A5M8V:1:2103:18839:26052/251‑1 (MQ=60)
               TCGAAAAGAAACGCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGAGCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTTTCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAAC                                                                                                                                                                                                                             <  M01186:129:000000000‑A5M8V:1:2114:21950:4444/251‑1 (MQ=60)
                 cgaaagtaactcggaaCGAAACGCCGGAAAATCCCCCCGGGCGGCGATCTGGCACCGGGCTGGCTGAAGATTGGTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGGCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACAT                                                                                                                                                                                                                           <  M01186:129:000000000‑A5M8V:1:1112:18731:25764/235‑1 (MQ=60)
                    AAGAAACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGG                                                                                                                                                                                                                        <  M01186:129:000000000‑A5M8V:1:1103:19969:17915/251‑1 (MQ=60)
                      AAAACTCGAAGCGAAACGCCGAAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATAGCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTC                                                                                                                                                                                                                      <  M01186:129:000000000‑A5M8V:1:1110:8435:23127/251‑1 (MQ=60)
                        AACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAA                                                                                                                                                                                                                    >  M01186:129:000000000‑A5M8V:1:1105:18488:16773/1‑251 (MQ=60)
                           CCGAAGCGAAACGCAGCAAACTCCCCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATC                                                                                                                                                                                                                 <  M01186:129:000000000‑A5M8V:1:2109:20027:24128/251‑1 (MQ=60)
                              AAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATAACTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACCTCGTACTGGACCCGCTGGGCGTACCGTCCCGTATGAACATCGGTCAGATCCC                                                                                                                                                                                                               >  M01186:129:000000000‑A5M8V:1:1102:26475:11135/1‑250 (MQ=60)
                                 GGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGA                                                                                                                                                                                                            <  M01186:129:000000000‑A5M8V:1:2114:16719:6533/250‑1 (MQ=60)
                                 CGAAACGCCGCAAAATCCCCCAGGCCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGA                                                                                                                                                                                                            <  M01186:129:000000000‑A5M8V:1:2113:10959:16720/250‑1 (MQ=60)
                                  aggcaGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGA                                                                                                                                                                                                            <  M01186:129:000000000‑A5M8V:1:1114:15849:10779/244‑1 (MQ=60)
                                             AAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATAGTACTGAACAAGCTGGGCTTACCGTCTCGTATGAACATCGCTCAGATCCTCGAAACCCACCTGGGA                                                                                                                                                                                               >  M01186:129:000000000‑A5M8V:1:2111:21316:4922/1‑251 (MQ=60)
                                                  CCCCAGGGCGACGATCGGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGC                                                                                                                                                                                          <  M01186:129:000000000‑A5M8V:1:2101:12033:5590/251‑1 (MQ=60)
                                                               ATCTGGCATCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACACGATGGCAGGTCGTCACGGTAACCAGGGAGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGAACGCCGGCAGCCATCGTACTTAACCAACTGGGCTGACCGGCCCGTCTGAACATCGGCCAGAGCCCCGGAACCCACCTAGGAATGGCTGGGAAAGGGAT                                                                                                                                                                              >  M01186:129:000000000‑A5M8V:1:1103:2577:17877/1‑250 (MQ=60)
                                                               ATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTAT                                                                                                                                                                              >  M01186:129:000000000‑A5M8V:1:1104:8034:12833/1‑250 (MQ=60)
                                                               ATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGAAT                                                                                                                                                                              >  M01186:129:000000000‑A5M8V:1:1108:8879:9633/1‑250 (MQ=60)
                                                                   GGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGAATGGCTGgcaaagggagcggcg                                                                                                                                                                         >  M01186:129:000000000‑A5M8V:1:1111:18797:7650/1‑236 (MQ=60)
                                                                                 TGAAGATTTTTAAGGTATAGCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGC                                                                                                                                                            <  M01186:129:000000000‑A5M8V:1:2110:24470:8506/250‑1 (MQ=60)
                                                                                  GAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGCTCAACGCCA                                                                                                                                                          >  M01186:129:000000000‑A5M8V:1:2112:16718:12081/1‑251 (MQ=60)
                                                                                       tttgtaagggagATCTGGCGGTTAAACGGCGTATCCAGACTGGTGACAAGATGGCAGGGTGGAAAGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTCGACATCGTACTGAACCAGCTGGGCGTACCGTCCCGTATGAATATCGGTCAGACCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGA                                                                                                                                                       <  M01186:129:000000000‑A5M8V:1:2102:4139:10413/237‑1 (MQ=60)
                                                                                           TAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAC                                                                                                                                                 <  M01186:129:000000000‑A5M8V:1:2102:25085:19570/251‑1 (MQ=60)
                                                                                           TAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAC                                                                                                                                                 <  M01186:129:000000000‑A5M8V:1:1109:16163:16510/251‑1 (MQ=60)
                                                                                              GGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGCTATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGCCTCGTATGAACATCGGTAGGATCCTCGAAACCCACCCGGGAATGGCTGCGAAAGGGATCGGCGcaaaaatccacgccatggtgaaaacga                                                                                                                                              >  M01186:129:000000000‑A5M8V:1:1107:12152:22289/1‑224 (MQ=60)
                                                                                                     TTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTAACGGTAACAAGGGTGTAATTTCTAACATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGA                                                                                                                                       <  M01186:129:000000000‑A5M8V:1:2113:13272:15071/251‑1 (MQ=60)
                                                                                                           GTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGGATCGGCGCCAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCcg                                                                                                                                 >  M01186:129:000000000‑A5M8V:1:1103:17197:14992/1‑249 (MQ=60)
                                                                                                           GTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGAATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCG                                                                                                                                  >  M01186:129:000000000‑A5M8V:1:2112:22686:17013/1‑250 (MQ=60)
                                                                                                           GTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGAATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCG                                                                                                                                  >  M01186:129:000000000‑A5M8V:1:2112:19612:23496/1‑250 (MQ=60)
                                                                                                                  GCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCG                                                                                                                                                                                                                                         >  M01186:129:000000000‑A5M8V:1:1111:21379:24395/1‑140 (MQ=60)
                                                                                                                     GTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACACAAGCAAGAAGTCGCGAAACgcccg                                                                                                                       >  M01186:129:000000000‑A5M8V:1:1110:4291:10269/1‑246 (MQ=60)
                                                                                                                      TATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCCAGAAGCGCCGAAACTGCGCG                                                                                                                      >  M01186:129:000000000‑A5M8V:1:2102:17730:22642/1‑251 (MQ=60)
                                                                                                                        TCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAATCCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAA                                                                                                                    <  M01186:129:000000000‑A5M8V:1:1108:10676:11602/251‑1 (MQ=60)
                                                                                                                                     CAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTAATCCAGCGTG                                                                                                       >  M01186:129:000000000‑A5M8V:1:1111:16726:9135/1‑251 (MQ=60)
                                                                                                                                               GGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATC                                                                                              <  M01186:129:000000000‑A5M8V:1:1101:28271:17378/250‑1 (MQ=60)
                                                                                                                                                    CCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCG                                                                                        <  M01186:129:000000000‑A5M8V:1:1105:26404:24112/251‑1 (MQ=60)
                                                                                                                                                     CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAT                                                                                                                                                                                                                                 <  M01186:129:000000000‑A5M8V:1:1111:17393:23417/113‑1 (MQ=60)
                                                                                                                                                       CGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCG                                                                                        >  M01186:129:000000000‑A5M8V:1:1103:16465:18192/1‑248 (MQ=60)
                                                                                                                                                       CGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCACGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGGGCGTACGCTCTGGGCGCga                                                                                     >  M01186:129:000000000‑A5M8V:1:2111:17379:13835/1‑249 (MQ=60)
                                                                                                                                                       CGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCCGGAATTCATCCAGCGTGCGTACGATCTGGGCG                                                                                        >  M01186:129:000000000‑A5M8V:1:1102:14480:27119/1‑248 (MQ=60)
                                                                                                                                                         GTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGGGCGTACGATCTGGGCGCTGA                                                                                    >  M01186:129:000000000‑A5M8V:1:2111:9687:17082/1‑250 (MQ=60)
                                                                                                                                                          TAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTAATCCACCGTGCGTACGATCTGGGCGCTGACG                                                                                  >  M01186:129:000000000‑A5M8V:1:1106:19309:22652/1‑251 (MQ=60)
                                                                                                                                                             CAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTAGGATCTGGGGGCTGACGTTG                                                                               >  M01186:129:000000000‑A5M8V:1:2112:26495:19603/1‑251 (MQ=60)
                                                                                                                                                                      AATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑AGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAG                                                                      <  M01186:129:000000000‑A5M8V:1:1107:24522:19882/251‑1 (MQ=60)
                                                                                                                                                                       tgtataagagacctCCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGT                                                                     <  M01186:129:000000000‑A5M8V:1:2109:7499:18752/237‑1 (MQ=60)
                                                                                                                                                                          TCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTAATCCAGCGTGAGGACGAACTGGGGGCTGACGTTGGTCAGAACGTTGA                                                                  >  M01186:129:000000000‑A5M8V:1:2102:15905:18718/1‑251 (MQ=60)
                                                                                                                                                                            TAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACC                                                                <  M01186:129:000000000‑A5M8V:1:1103:24063:8178/251‑1 (MQ=60)
                                                                                                                                                                                ATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGCCGTTCGTCAGAAAGTTGACCTGCG                                                            >  M01186:129:000000000‑A5M8V:1:2110:15065:21595/1‑251 (MQ=60)
                                                                                                                                                                                  CAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCCGAAAGTTGACCTGAGTA                                                          >  M01186:129:000000000‑A5M8V:1:2104:23990:10303/1‑251 (MQ=60)
                                                                                                                                                                                   AACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTAC                                                         <  M01186:129:000000000‑A5M8V:1:2104:25115:19208/251‑1 (MQ=60)
                                                                                                                                                                                      CCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTT                                                      <  M01186:129:000000000‑A5M8V:1:1114:17422:14255/251‑1 (MQ=60)
                                                                                                                                                                                       cgaactagaATATGCCTTA‑CGGTGAAAACGGTACGCCGGTATACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCCCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTC                                                     <  M01186:129:000000000‑A5M8V:1:2104:24810:23311/242‑1 (MQ=60)
                                                                                                                                                                                         ATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTC                                                                            >  M01186:129:000000000‑A5M8V:1:1112:19858:25030/1‑226 (MQ=60)
                                                                                                                                                                                             AAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGCAGTCGCGAAACTGCGCGAATTCATCCACCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGcg                                               >  M01186:129:000000000‑A5M8V:1:1111:25560:12674/1‑249 (MQ=60)
                                                                                                                                                                                             AAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGCGTACCTTCAGCG                                                 >  M01186:129:000000000‑A5M8V:1:2101:18999:4369/1‑249 (MQ=60)
                                                                                                                                                                                                 TATGCCTTACCGAT‑AAAGCGGTACGCCGGTAGGCGTCGGACTGAACCAGCCGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTAACTTCAGCGATGAAG                                           <  M01186:129:000000000‑A5M8V:1:1101:6332:23720/251‑1 (MQ=60)
                                                                                                                                                                                                    GCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGAATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGGCCTGACGTTCGTCCGAAAGTTCACCTGAGGACCTTCAGCGATGACGAAG                                        >  M01186:129:000000000‑A5M8V:1:2112:13648:24647/1‑251 (MQ=60)
                                                                                                                                                                                                      CTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTT                                      <  M01186:129:000000000‑A5M8V:1:1114:10436:9687/251‑1 (MQ=60)
                                                                                                                                                                                                            CATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCG                                 <  M01186:129:000000000‑A5M8V:1:1113:18820:14851/251‑1 (MQ=60)
                                                                                                                                                                                                             ggctcgggaatgtgtataagagacagGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGTCCTGAGTACCTTCAGCGATGAAGAAGTTATGCG                                 <  M01186:129:000000000‑A5M8V:1:2111:14911:18758/224‑1 (MQ=60)
                                                                                                                                                                                                                           CCGGGAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACC                   <  M01186:129:000000000‑A5M8V:1:1101:3004:9704/250‑1 (MQ=60)
                                                                                                                                                                                                                              GTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGC                >  M01186:129:000000000‑A5M8V:1:1114:24545:8246/1‑250 (MQ=60)
                                                                                                                                                                                                                                  ACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACATGCGCAAA           >  M01186:129:000000000‑A5M8V:1:2101:15819:9255/1‑251 (MQ=60)
                                                                                                                                                                                                                                       GTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCCCAAAGGTAT      >  M01186:129:000000000‑A5M8V:1:2112:16689:26221/1‑251 (MQ=60)
                                                                                                                                                                                                                                       GTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGAACGATCTGGGCGCTGACGTTCGTCAGAAAGTTAACCTGGGTACCTTCAGCGATGAAGAAGTTATGCGTCCGGCGGAAAACCTGCGCAAAGGaag      >  M01186:129:000000000‑A5M8V:1:2110:11145:20902/1‑248 (MQ=60)
                                                                                                                                                                                                                                       GTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACAGCGCGAATTCATCCAGCGTGCGAACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTAAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTCCGCAAAGGTAG      >  M01186:129:000000000‑A5M8V:1:1102:15570:26514/1‑251 (MQ=60)
                                                                                                                                                                                                                                       GTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGGCGCGAAACAGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTGCGCCAGCAAGTTGACCTGAGTACCTTCAGCAATGAAGAAGTTATGCGTCTGGCTGAAAAACTGAGCAAAGGTAT      >  M01186:129:000000000‑A5M8V:1:2102:24146:8577/1‑251 (MQ=60)
                                                                                                                                                                                                                                         TCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGC    <  M01186:129:000000000‑A5M8V:1:2113:24653:23984/251‑1 (MQ=60)
                                                                                                                                                                                                                                           TGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCA  <  M01186:129:000000000‑A5M8V:1:2110:18404:6651/251‑1 (MQ=60)
                                                                                                                                                                                                                                           TGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCA  <  M01186:129:000000000‑A5M8V:1:1101:26289:18605/251‑1 (MQ=60)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    
AACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTA‑CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCA  >  NC_000913/4184303‑4184787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑