Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F9 I0 R1 177 96.4 2178490 87.4% 1904000 226.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,090,130 A→C 25.4% T126P (ACC→CCC)  yggI → Zn‑dependent metalloprotease‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,090,1300AC25.4% 198.6 / 19.8 104T126P (ACC→CCC) yggIZn‑dependent metalloprotease‑related protein
Reads supporting (aligned to +/- strand):  ref base A (56/15);  new base C (4/28);  total (60/44)
Fisher's exact test for biased strand distribution p-value = 1.73e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

CGCCGGAACGGCCTGGCTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTAT  >  NC_000913/3089892‑3090358
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                     
cgccgGAACGGCCTGGCTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAaca                                                                                                                                                                                                                                       <  1:1079440/239‑1 (MQ=255)
               gctggAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGGGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCCACACCTTCCCCTACCGc                                                                                                                                                                                                                         >  1:438943/1‑238 (MQ=255)
                    aaaGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGCAAACGGGCTGGGAGTTCCCACCCGT‑CGTACGCATCAGTTCGAACT‑CCACTCCGTGCGTCGCAACACCTTCCCCTACCGCTGCacg                                                                                                                                                                                                                   >  1:502548/1‑237 (MQ=255)
                    aaaGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAg                                                                                                                                                                                                                   >  1:934457/1‑239 (MQ=255)
                     aaGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGGGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGGGCGTCGCAACCCCTTCCCCTACCGCTGCAAg                                                                                                                                                                                                                   >  1:601191/1‑238 (MQ=255)
                     aaGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGGGCTGGGTGTTCCCGCCACG‑CGCACGCCCAAGTTCGAACT‑GCAATCCGTGCGCGACAACACCTTCCCCAACCGCGGCAAGt                                                                                                                                                                                                                  >  2:729876/1‑239 (MQ=255)
                        cTATGAAATTCCCCTCAACCCCGTTTTGCTGTTGGAAAACAGTTAAGCTTTTAATGAAGAAGTGGTACCTCACGAACGGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGcc                                                                                                                                                                                                               <  1:822691/239‑1 (MQ=255)
                          tttaaaTGCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTTAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCa                                                                                                                                                                                                              <  1:773781/235‑1 (MQ=255)
                          aagAAATTCGCCTCAATCCCGTTTTGCGGTTGGAAAACAGTGAAGCTGTTATTGAAGAAGTGGTTCCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCa                                                                                                                                                                                                              <  1:1063853/236‑1 (MQ=255)
                          aTGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACCCCTTCCCCTACCGCTGCAAGTGCCAg                                                                                                                                                                                                             >  1:169835/1‑239 (MQ=255)
                           tGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAgg                                                                                                                                                                                                            <  1:8948/239‑1 (MQ=255)
                               aTTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCa                                                                                                                                                                                                        <  1:673235/239‑1 (MQ=255)
                                  cGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTGGCAACCCCTTCCCCCACCCCCGCAAGGGCCAGGGCCATCa                                                                                                                                                                                                     >  2:871325/1‑239 (MQ=255)
                                  cGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTACCCTACCGCTGCAAGTGCCAGGCGCATCa                                                                                                                                                                                                     >  1:258783/1‑239 (MQ=255)
                                  cGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGCGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTCCCGCTGCAAGTGCCAGGAGCATc                                                                                                                                                                                                      >  1:880108/1‑238 (MQ=255)
                                   gCCTCAAACCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGACAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAg                                                                                                                                                                                                    <  1:122829/239‑1 (MQ=255)
                                     cTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGGTCCCGCCCGT‑CGTACGCATCAGTTCGACAT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTCCAAGTGCCAGGAGCATCAGCt                                                                                                                                                                                                  >  2:285029/1‑239 (MQ=255)
                                                    cTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGGGTTCCCGCCCGC‑CGTCCGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTACCCTACCGCTGCAAGGGCCAGGGGCATCAGCTTACC‑GACCGCCGCCa                                                                                                                                                                                  >  1:1002965/1‑239 (MQ=255)
                                                        tGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATaa                                                                                                                                                                               >  1:871902/1‑238 (MQ=255)
                                                        tGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACGGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAGCGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAAt                                                                                                                                                                              <  1:866944/239‑1 (MQ=255)
                                                         ggAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCAACCCCTGCACGTGCCGGGGGCATCAGCTTACC‑GTCCGCCGCCATaacc                                                                                                                                                                             >  1:393784/1‑237 (MQ=255)
                                                                ctggaactttttATTGAAGAAGGGGTCCCGACCGACCGGGCAGTTTTGGT‑GGTATGAAAACATTGGGGCCGGGTGGCGCCACAGGGCAAAGA‑GGGGAAGGGGATAATGGAAAACGTGCGGGGTGTCCCCGCCCTG‑CGTACGCATAAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAGGTGCCGGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGt                                                                                                                                                                      <  2:989086/231‑1 (MQ=255)
                                                                 gTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAgg                                                                                                                                                                      >  1:1007080/1‑237 (MQ=255)
                                                                 gTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGt                                                                                                                                                                      >  1:958271/1‑238 (MQ=255)
                                                                 gTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGGGCCAGGAGCATCAGCTTACC‑GTACCCCCCCCAAATCGCGAAGtt                                                                                                                                                                     >  2:880477/1‑239 (MQ=255)
                                                                           tATTGAAGGAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACCCCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAgg                                                                                                                                                           <  1:871325/239‑1 (MQ=255)
                                                                                aagaagTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTCACGCTGCAAGTGCCAGAAGCATCAGCTTACC‑GCACGCCGCCATAATCGCGCGGTTCATGGCAAGGCGGTc                                                                                                                                                      >  1:106217/1‑239 (MQ=255)
                                                                                       ggTCCCGCACGAACTGGCACATTTGGT‑GGTAGGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGAGGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACCCCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCt                                                                                                                                               <  1:1081059/239‑1 (MQ=255)
                                                                                              cacaAACTGGC‑CATTTGGTGGGTAGGAAACCTTTTGGGGCGGGAACCGCCAC‑GGGGAAAGAGGTGGAATGGGGTAATGAAAACCGTGCTGGGTGTCCCCGCCCGT‑CGTCCGCACCAGTTCGAACT‑GCAACCCGTGCGTCGAAACCCCTCCCCCTACCGCTGCAAGGGCCAGGAGCATCAGCTTCCC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCa                                                                                                                                        <  2:287991/239‑1 (MQ=255)
                                                                                              cACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGGGCATCAGCTTACC‑GTACGCCGCCATAATCGCGGAGTTCGGGGCGAGGCCGGCTATCGCCGTGTTc                                                                                                                                         >  2:258428/1‑238 (MQ=255)
                                                                                              cACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCCACCGCTCTGttt                                                                                                                                         >  1:100129/1‑237 (MQ=255)
                                                                                              cACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGCCTATCGCTGTGTTc                                                                                                                                         >  1:114114/1‑238 (MQ=255)
                                                                                                cGAACTGGCACATTTGCT‑GGTATGGCAACATTTCGGCCGCGTAGCGCCACATGGCAAAGC‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACCCCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACCCCCCCATAATCGCTTAGTTCGTGCCGAGGCCCTCAACCGCCgtgtccccc                                                                                                                                      >  2:662380/1‑234 (MQ=255)
                                                                                                cGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGGGCATCAGCTTACC‑GTACGCCGCCATAATCCCGTAGTTCGGGGCGAGGCCGTCACACGCTGGGTTcccc                                                                                                                                      >  2:400636/1‑236 (MQ=255)
                                                                                                 gcttgggCACTTTTGTT‑GGTTGGGAAAAGTTTGGGCCGCGGAGCCCCCAATGGGAAGGG‑AGGGAGGGGGATGAGGGAAAACGTGCGGGGTGTCCCCGCCCGG‑CTTCCGCTGGAGTTCGACCT‑ACAACCCGTGCGTCGCAACCCCTCCCCCTACCGCTGCAAGTGCAAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTg                                                                                                                                     <  2:258783/234‑1 (MQ=255)
                                                                                                 gAACTGGAACTTTTGTT‑GGTATGGAAACATTTCGGCCGGGTAGCGCCACAGGGCAAAGA‑GTGGAAGGGGATGATGGAAACCGTGCTGGGTGTTCCCGCCCGC‑CGTCCGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTg                                                                                                                                     <  1:274619/239‑1 (MQ=255)
                                                                                                   aCTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGcc                                                                                                                                   >  1:1073185/1‑238 (MQ=255)
                                                                                                   aCTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCCCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCCTAATCCCGGAGTTCGTGGCGGGGCCGCCGATCGCGGTGTTccatggg                                                                                                                                   >  2:565403/1‑233 (MQ=255)
                                                                                                       gCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGCCTATCGCTGTGTTCAcgtcctgt                                                                                                                                >  1:491871/1‑231 (MQ=255)
                                                                                                       gCACATTGGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGGGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCg                                                                                                                                                 <  1:700916/221‑1 (MQ=255)
                                                                                                        cacaTTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTGGTTCGTGGCGAGGCGGTCTACTGCTTCGTTCACTGCGGTGa                                                                                                                               >  1:613486/1‑238 (MQ=255)
                                                                                                        cacaTTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGGGa                                                                                                                               >  1:287991/1‑238 (MQ=255)
                                                                                                             ttGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGCAGTTCGTGGCGAGGCCGTCGATCTCTGTGTTCCCTTCGGTGAACa                                                                                                                            >  1:671094/1‑236 (MQ=255)
                                                                                                             ttGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGGGCTGGGTGTTCCCGCCCGT‑CGAACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTCGTTCGTGGCGAGGCCGTCATCCGCTGTGTTCACTGCGGTGac                                                                                                                              >  1:862196/1‑233 (MQ=255)
                                                                                                               gCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCCCTGGGTTCACTGCGGGGAACAGCggg                                                                                                                       >  2:955890/1‑239 (MQ=255)
                                                                                                                   ggTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTTTCGCTGTGTTCCCTGCGGTGAACAGCTGGTTg                                                                                                                    >  1:224021/1‑239 (MQ=255)
                                                                                                                       tGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAAAAGCGGGTTGCGaa                                                                                                                >  1:857740/1‑239 (MQ=255)
                                                                                                                           aaCATTTGGCGCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTTCGGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACCCCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATaa                                                                                                            <  1:565403/239‑1 (MQ=255)
                                                                                                                               tttCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTCCGGTGAACAGCTGGTTGCGAAATAACCa                                                                                                         >  1:86356/1‑238 (MQ=255)
                                                                                                                                        cgTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCTTAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGGGAACAGCTGGTTGTGAAATAACCACCTGAACtc                                                                                                >  1:945315/1‑237 (MQ=255)
                                                                                                                                        cgTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGCCTATCGCGGTGTTCCCTGCGGGGACCAGCGGGGGGCGAAAAAAACATCTGaaaaat                                                                                               >  2:356582/1‑235 (MQ=255)
                                                                                                                                        cgTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGGGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGCAGTTCGGGGCGAGGCCGTCTACTGCTGTGTTCACTGCGGGGACCGGCTGGTGTGGGAATAACCTACTGACCtc                                                                                                >  2:709291/1‑237 (MQ=255)
                                                                                                                                              gcCACATGGCAAAGA‑GTTGAAGTGGATGATGGAAAACGTGCTGGGTTTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACCCCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAgg                                                                                           <  1:356582/237‑1 (MQ=255)
                                                                                                                                              gcCAAATGGCAAAGA‑GTGGAAGTGGATGATGGAAACCGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACCCCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGaa                                                                                         <  1:885662/239‑1 (MQ=255)
                                                                                                                                                  caTGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCCAGGCCCTCTATCACTGTGTTCCCTGCGGGGAACAGCTGGTTCCGAAATAACAACCTGAACTCTCAGGAAcct                                                                                      >  1:1050039/1‑236 (MQ=255)
                                                                                                                                                     ggccaaggggtggatgtggtGGTTGAAAATGGTGCGGGGTGTACCCCCCCGC‑CGTACCCATCGGTGCGAACT‑GCAATCCGGTCGTCGCACCCCCCCCCCCACCCGCTGCAAGGGCAGGGAGCATCAGCTCACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTcc                                                                                   <  2:300379/230‑1 (MQ=255)
                                                                                                                                                        aaaGG‑GTGAAAGTGTGTGATGGAAACGGGGCGGGGTGTTCCCGCCCGG‑TGTCAGCATCAGTACCAAGT‑GTATTCCTCGTGTCGCACACCCGTCCCCCACTGCGGCAAGTGCCTGGAGCATCGTCTTACC‑GAACGCCGCCATAATCGCGTAGTTCGGGGCGAGGCCGTCTATCGCTGTGTTCTCTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGGACTATCAGGAACTTTCCTGAt                                                                               <  2:756410/239‑1 (MQ=255)
                                                                                                                                                            a‑gtggaagtggaTTATGGAAACGGTGCTGGTGGTTCCCGCCCGT‑CGTACCCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGAt                                                                               <  1:627603/235‑1 (MQ=255)
                                                                                                                                                                ggaagtggaTGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTg                                                                            >  1:30333/1‑235 (MQ=255)
                                                                                                                                                                ggaagtggaTGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTACGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTg                                                                        >  2:408362/1‑239 (MQ=255)
                                                                                                                                                                 gaagtggaTGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACCCCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTa                                                                                                                                                    <  1:158387/162‑1 (MQ=255)
                                                                                                                                                                 gaagtggaTGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTa                                                                                                                                                    >  2:158387/1‑162 (MQ=255)
                                                                                                                                                                 gaagtggaTGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGCGTTCACTGCGGTGAACAGCTGGTTGCGAAAAAACCAACTGAACTATAAGGAACTTTCCTGAACAGGCTGa                                                                       >  2:20937/1‑239 (MQ=255)
                                                                                                                                                                  aagtggaTGATGGAAAAAGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTCCGGTGACCAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGAt                                                                      >  1:1039764/1‑239 (MQ=255)
                                                                                                                                                                      ggtgggtgGAAACCGGGGTGGGT‑TTCCCCACCGTCCGTACCCATCTGTTCGAACT‑CCAATCCCGGCGTCCCACCCCCCTCCCCTCCCGCTGCAAGTGCCAGGAGCATCATCTTACC‑GTCCGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCa                                                                  <  2:30333/233‑1 (MQ=255)
                                                                                                                                                                      ggaTGAGAGAAACCGTGCTGGGTGTTCCCCCCCGT‑CGTACGCATCAGTGCGAACT‑GCAATCCGTGCGTCGCAACCCCTGCCCCTACCGCTGCAAGTGCAAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCa                                                                  <  1:779472/239‑1 (MQ=255)
                                                                                                                                                                       gatgatGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGCGGCCGTCTATCGCTGTGTTCACTGCGGGGAAAGACTGGGTGCGAAATAACCATCTCAACTATCAGGAACTTTCCTGACCTGGCTGAGTgggg                                                                 >  2:627603/1‑236 (MQ=255)
                                                                                                                                                                       gatgatGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACt                                                                                                 >  1:61036/1‑207 (MQ=255)
                                                                                                                                                                       gatgatGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCAt                                                                 >  1:188500/1‑239 (MQ=255)
                                                                                                                                                                            tgGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCt                                                                                                                                               <  1:665810/156‑1 (MQ=255)
                                                                                                                                                                            tgGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCt                                                                                                                                               >  2:665810/1‑156 (MQ=255)
                                                                                                                                                                            tgGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGGGAACAGCTGGTTGCGAAATAACCCTCTGACCTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCaa                                                            >  1:44581/1‑239 (MQ=255)
                                                                                                                                                                                       cTGGGCGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCCGCTTACC‑GTACGCCGCCATACTCGCGTGGGTCGTGGCGAGGCCGGCTCTAGCGGTGGTCATCGCGTTGAATAGCTGGTTGCGAAATAACCCTATGAACTATCCGGAAATTTCCTGACATGGCTAAGTGCCTACCAAAAAAGCttgc                                                  >  2:953953/1‑236 (MQ=255)
                                                                                                                                                                                         gggTTTTCCCGCCCGC‑CGTACGCATCAGTTCGAACG‑GCAATCCGTGCGTCGCAACACTTTCCCCAACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCt                                               <  1:928715/239‑1 (MQ=255)
                                                                                                                                                                                          ggTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCCTAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGCGAACAGCTGGTTGCGAAATAACCATCTGAACTTTCAGGAACTTTCCGGATCTGGCTGATTGCAAACCAAAACAGCTTTCGCtc                                              >  2:928715/1‑238 (MQ=255)
                                                                                                                                                                                               tCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCTTACCAAAACAGCTTTCGCTACGta                                          >  1:741512/1‑237 (MQ=255)
                                                                                                                                                                                                      cgt‑cgtACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGGGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTAGCTACGTTGCTGGCTc                                  >  1:867239/1‑239 (MQ=255)
                                                                                                                                                                                                      ccgtgctccGCACCAGTCCGAACT‑GCAATCCGTCCGTCGCAACCCCTTCCCCTACCGCTGAAAGTGCCGGGAGCATCAGCTTCCC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCt                                   <  1:139015/231‑1 (MQ=255)
                                                                                                                                                                                                                catcatTTCGCACT‑CCAACCCGGGCGGCGCCCCCCCCCCCCCTCCCGCTCAAAGTGCCAGGGCAATCAGCTTACC‑GGACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAaca                         <  2:958271/239‑1 (MQ=255)
                                                                                                                                                                                                                cATCAGTTCGAACT‑GCCATCCTTGCTTGGCACCCCCTCCCCCGCCCCTTGAAAGGGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTACCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAaca                         <  2:409549/239‑1 (MQ=255)
                                                                                                                                                                                                                cATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGACCTATCAGGAACTTTCCTGATCTGGCGGATTGCATACCAAACAAGCTTTAGCTACCTTGCTGGCTCGTTTTAac                          >  1:443397/1‑238 (MQ=255)
                                                                                                                                                                                                                 aTCAGTGCGAACT‑CAAATCCGTGCGCCCCACCTCTTCCCCCTACCGGTGCAGGTGCCAGGAGCATCAGCTTACC‑GTGCGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAacac                        <  1:709291/239‑1 (MQ=255)
                                                                                                                                                                                                                      ttCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGTTTGCCTACCAAAAAAACTTTCCCAACGTTGCTGGCCCGTTTTAACCCgggga                   >  2:465451/1‑236 (MQ=255)
                                                                                                                                                                                                                      tccggccT‑GCACTCCGGGCGGCGCACCCCCTCCCCCGCCCGGCGCCAGTGCCGGGGGCACCGGCTTCCCTGTA‑GCCGCCATTATCGCGTAGTTCGTGGCGAGGGCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGt                   <  2:748622/233‑1 (MQ=255)
                                                                                                                                                                                                                       tcaaaCT‑GCAATCCTGGCGTCCCAACCCCTTCCGCTACCGTTGCAAGTGCCGGGAGCAGTAGCTTCCC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGt                   <  1:131059/235‑1 (MQ=255)
                                                                                                                                                                                                                       tCGAACG‑GCAATCCGGGCGTCCCAACCCCTCCCCCTACCGCTGCAAGTCCCAGGAGCATCAGCTTCCC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGt                   <  1:932916/238‑1 (MQ=255)
                                                                                                                                                                                                                       ccaaaCT‑GCAATCCGGGGGCACCACCCCCCCCCCCTCCCGCTGGACGGGCCAGGGACATCCTCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGGGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTAGCTGGCTCGTTTTAACACGGAGt                   <  2:741512/235‑1 (MQ=255)
                                                                                                                                                                                                                       ccaaaCT‑GAACTCCGTGCGGCGCAACCCCTGCCCTCACCCTCGAAAGTGCCAGGCGCAACAGCTTACC‑GTCCGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGt                   <  1:468555/235‑1 (MQ=255)
                                                                                                                                                                                                                        tgAACTGGCAATCCGGGCCTCGCAACCCCTCCCCCGACGCCGGAAGGGGCCGGGAGCGTCGGCTTACG‑TCACTCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGt                   <  2:871902/237‑1 (MQ=255)
                                                                                                                                                                                                                        cGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTaa                 <  1:408362/239‑1 (MQ=255)
                                                                                                                                                                                                                         ccatcggCAACCCGTGCGCCCCACCCCCTTCCCCTCCCGCTGCAAGTGCCAGGAGCATCAGCTTCCC‑GTACGCCGTCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGt                   <  1:1058973/231‑1 (MQ=255)
                                                                                                                                                                                                                           aCT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAAAAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAAACGCTTTCCCTACGTTGCTGGCCCGTTTTAAAACGGAGaaaaag              >  2:554205/1‑236 (MQ=255)
                                                                                                                                                                                                                            acggCAACCCGTGCGTCGCAACCCCTTCCCCTTCCCTTGCAAGTGTCAGGAACAGCCGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCAGACCAAAACAGCTTGCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTg              <  1:287064/236‑1 (MQ=255)
                                                                                                                                                                                                                             t‑GCAACCCGGGCGTCGCAACCCCTTCCCCTCCCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAaca                         <  1:178114/226‑1 (MQ=255)
                                                                                                                                                                                                                               gccaTCCGTGCGTCCCAACCCCTTCCCCTACCGGTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATg           <  1:955890/236‑1 (MQ=255)
                                                                                                                                                                                                                                cAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCCACATTGCTGGCTCGTTTTAAAACGGTGGAAggagtgc          >  2:973316/1‑233 (MQ=255)
                                                                                                                                                                                                                                cAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATGGCATACCAAAACAGCTTCCGCTACGTTGCTGGCTCGTTTTAACCCGGGGAAAGGGATg           >  2:527170/1‑238 (MQ=255)
                                                                                                                                                                                                                                cAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGACCTGGCTGATTGCATACCAAAACAGCTTTCGCAACGTTGCTGGCCCGTTTTAAAACGGGGAAAGGGagtt          >  2:932916/1‑236 (MQ=255)
                                                                                                                                                                                                                                 aaTCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCCCGTTTTAACACGGAGTAAGGGATgg          >  1:807735/1‑237 (MQ=255)
                                                                                                                                                                                                                                        gCGTCCCAACCCCTTCCCCTCCCGCTGCAGGTGCCAGGGCCATCAGCTTACG‑TTACGCCGCCATAATCGCGTAGGTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTAt  <  1:20937/239‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                     
CGCCGGAACGGCCTGGCTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCT‑GGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGA‑GTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGT‑CGTACGCATCAGTTCGAACT‑GCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACC‑GTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTAT  >  NC_000913/3089892‑3090358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A