Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F17 I0 R1 49 108.0 2318666 92.3% 2140128 226.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,506 C→A 14.4% T15K (ACA→AAA)  ttdA → L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,5060CA14.4% 136.0 / 2.3 63T15K (ACA→AAA) ttdAL‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (20/29);  new base A (2/7);  total (25/38)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTT‑TTAGTGGTAGTTTGTGGCGGTGAATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAA  >  NC_000913/3206281‑3206744
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               
aGCGCAGTGTATTACGCAAAACATTGCCATTCCCTGCTCTGCCGCATTATTCATTCTTATTTTCGTTTTGATTCACGAACTGTTAATTAAATATTCACGGTGGCAAAAAATATAAAACCACATTT‑TTAGTGGTAGTTTGGGGCGGGGAATTTTTCCA‑GACAAAGACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAGAGTGTCGCt                                                                                                                                                                                                                                    <  2:302195/239‑1 (MQ=255)
            ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTT‑TTAGTGGTAGTTTGTGGCGGTGAAATTTTTCC‑GACAAATACAAAAAATGGAGTTGTCCTGATGAGAGAAAGGAAAAAACAACAAGCAGGG‑AATAAGTTTACAGAGATTGTTGCTAACTTTAACGc                                                                                                                                                                                                                         >  1:906499/1‑238 (MQ=255)
             gccaaaaaatttGCCATTCCATTCTGTGGCGAAAAATTCATTTTTAGTCTGGTGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCCCTATT‑TTAGTGAAAGTTAGTGGGGGGAAATTTTCCAA‑TACAAATACAAAATGTGTAGTTGCGTAAATGGGCAAAAGTAATAAGAAACAGGAGGAT‑TCTAAGTTGTGAGGATTTTTCGTTAACTTTACCGCCa                                                                                                                                                                                                                       <  2:586948/229‑1 (MQ=255)
                        tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTT‑TTAGTGGTAGTTTGTGGCGGGGAAATTTTTCA‑GAAAAAAAAAAAAAAATGAGGTTGCATGATTAGGGAAAGTAATAAACAACACACAGAG‑AAAAAAGTTACAGAGATTTTTTCTTACCTTTCCGCCATGAttttcta                                                                                                                                                                                                             >  2:60242/1‑234 (MQ=255)
                         gCCATCCCCTGCCCTGGCGCAATATTCATTCTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTT‑TTAGTGTTAGTTTGTGGCGGTGAATTTTTCCA‑GACAAATAAAAAAACGGGAGTTGCAATGATGAGAGAAAGTAATAAGAAACAGGCAGTA‑AATAAGTTGAAAGAGATTGTCGCTAATTTTACCGCCATGATTTCTAccc                                                                                                                                                                                                           <  1:1142186/239‑1 (MQ=255)
                             tcccttGCTCTGGCGCATTATTCAGTCTTATTTTCGCGTTGATTCACGACCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCATATTT‑TTAGTGGTTTGTTTTGGCGGTGATTTTTTCAA‑GAAAAATACAAAACTGGGAGTTGCCATGAGGTGCAAAATTAATAAGCAACAGGAAGTG‑AATAAGTGGACAGAGATTTCCGATAACTTTACCGCCATGATTTCTACCCGAAt                                                                                                                                                                                                       <  2:1106716/234‑1 (MQ=255)
                               cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTT‑TTAGTGGTAGTTTGTGGCGGTGAATTTTTTCA‑GACAAATAAAAAAAATGGAGGTGACATTATTGGCGGAAGGAATAAGGAAACAGCAGGG‑AATAAGTTGAGAAAGGTTGTTGCCTACTTTATCGCCCCGAttttttacccaatg                                                                                                                                                                                                      >  1:1109367/1‑227 (MQ=255)
                                ccggCTCTGGCTAAAATTTCATTCTTATTTCCGATTTAATTAACGAAGTTTTAATTAAATATTCACGGTGGAAAAAAATAAAAAACCACATTT‑TTAGTGGTATTTTGTGGTGTTAAATTTTTCAA‑GACAAATAAAAAAACGGGAGTTGCCATGATGGGCTAAAGTAATAAGCAACAGCCATTG‑AATAAGTTAACAGGGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGcc                                                                                                                                                                                                    <  2:497370/236‑1 (MQ=255)
                                               tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTT‑TTAGTGGTAGTTTGTGGGGGTGAATTTTTCCA‑GAAAAATACAAAAACTGGAGTTGCCATGATGAGAGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                                     <  1:943734/239‑1 (MQ=255)
                                                   cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTT‑TTAGTGGTAGTTTGTGGCGGTGAAATTTTTCA‑AACAAATACAAAAAAAAGAGGTGCCATGATGAGCGAAAGGAATAAAAAACAGGCAGAG‑AATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCAATATTTCTAACCCAATGCCTGATGACGCGGGGGGTaaa                                                                                                                                                                                 >  1:719784/1‑239 (MQ=255)
                                                        ttATTTTCGCGTTGATTCACGAACTTTTAATTACATATTCACGGTGGAAAAAAATAAAAAACCACATTT‑TTAGTGGTAGTTTGTGGCGGTGAATTTTTCCA‑GAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                                                            <  1:60242/239‑1 (MQ=255)
                                                        ttATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTT‑TTAGTGGTAGTTTGTGGCGGTGAATTTTCCCA‑GACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                                                            <  1:353221/239‑1 (MQ=255)
                                                         tATTTTCGCGTTGATTCACGAATTTTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCACATTT‑TTAGTGGTAGTTTGTGGCGGTGAATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTG‑AATAAGTTGACAGAGATTTTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                                           <  1:87828/239‑1 (MQ=255)
                                                         tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTT‑TTAGTGGTAGTTTGTGGCGGTGAAATTTTTCA‑GACAAATAAAAAAAATGGAGGTGGCATGATGAGCGGAAGGAATAAGCAAAAAGGAGAG‑AATAAGATGAAAGAGATTTTGGCTAAATTTTACGCCCTGATTTCTTACCCAAAGCCTGAGTAAGGGGGGGGAAAACTaaaa                                                                                                                                                                           >  1:964194/1‑239 (MQ=255)
                                                         tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTT‑TTAGTGGTAGTTTGTGGCGGAGAATTTTTTCA‑AGCAAAAATAAAAACTGGAGTTGCCGTGATGGGGGAAAGGAATAAACAAAAAGAAGGGAAATAAATGGA‑AGAAATTTTCGCTTAATTTACCCCCCTGATTTTTTCCCGGAAGACTGATTACGTTGGGGGTAAACAaaaa                                                                                                                                                                           >  1:142788/1‑239 (MQ=255)
                                                         aatttcggtcttGCTTCCCAAATTGTAAATTACATTTTCCGGGTGGAAAAAATAAAAAAACCACTTTT‑TTAGGGGTAGTTTGGGGCGGTGAATTTTTCCA‑GACAAATAAAAAACCGGGAGTTGCCATGATGAGCGAAATTAATAAGCAACAGGCAGTA‑AATAAGTTGACAGAGATTGTCGCAAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                                           <  2:780063/229‑1 (MQ=255)
                                                                    tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTT‑TTAGTGGTAGTTTGTGGCGGTGAATTTTTCCA‑GAAAAATACAAAAACTGGAGTTGCAATGATGAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                                     <  1:57911/218‑1 (MQ=255)
                                                                    tGATTCACAAACTGTTAATTACATATTCACGGTGGAAAAAAAAAAAAAACCACATTT‑TTAGTGTTATTTTGTGGCGGTAATTTTTTCCA‑AACAAATACAAAAACTGGAGTTGCCATAATGAGCAAAAGTAATAAGCAACGGGGAGTA‑AATAAGTTAACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGa                                                                                                                                                                <  1:193005/239‑1 (MQ=255)
                                                                           caaaCTGTGAATTACATATTCACGGCGGAAAAAAAAAAAAAACCACATTT‑TTAGTGGTAGTTGGTGGCGGGGAATTTTTCAA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAATTAATAAACAACAGGCAGTA‑AATAGGTCGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGaa                                                                                                                                                         <  1:859525/237‑1 (MQ=255)
                                                                               cttttAATTGCATTTTCCGGGTGGAAAAAAAAAAAAACCCACATTT‑TGTGGGGTAGTGTGGGGGGGTGATTTTTTCCA‑GAAAAAAAAAAAAACGTGAGTGGCCATGAGGAGCGAAAGTAATAAGCAAAAGGCAGTG‑AATAAGTTGACAGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgt                                                                                                                                                     <  2:906499/236‑1 (MQ=255)
                                                                                 gTTAATTACATATTCACGGGAAAAAAAAATATAAAACCACATTT‑TTGTGGGTAGTTGTTGGCGGTTAATTTTTCAA‑AACAAATACAAAAACTGGAGTTGCCAGGTGGAGCAAAAATAAAAAGCACTAGGCGTTA‑AATAAGTGGAAAGAGATTGTCGTTATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgtcg                                                                                                                                                   <  2:964194/239‑1 (MQ=255)
                                                                                  ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTT‑TTAGTGGTAGTTTGTGGCGGTGAATTTTTTCA‑GAAAAAAAAAAAAAATGGAGGTGCCATGATGAGCGAAAGTAATAAACAACAAGCAGTG‑AATAAAGTTACAGAGATTGTCGCTAACTTTACCGCCATGATTTTTTACCGAATGCATGTTGACGTGGTGGGTAAAATAAAAAAACTAAAAGATGGCGCAAcgtcg                                                                                                                                                   >  1:925507/1‑238 (MQ=255)
                                                                                    aaTGACATGTTCCCGGTGGAAAAAAATAAAAAACCACATTT‑TAGGGTGTAGTTTGGGACGGTGAATTTTCCCA‑AAAAAATAAAAAAACTGGAGTTCCAGTAATGCGCAAAAGTAAAAACAAACGGGCAGTA‑AATAATTGGACAGAGTTTGCCTCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGtcgtcg                                                                                                                                                <  2:142788/239‑1 (MQ=255)
                                                                                      ttACATATTCGCGGGGGAAAAAAATAAAAAACCACATTT‑GTATTGGTGTTTTGTGGCGGTGAATTTTTCCA‑AAAAAAAAAAAAACCGGGAGTTTCAATGAAGAGAAAAAGTAAAAACAAAGGGGCAGTA‑AATAATGGGAAAGAGTTGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGtcgtcg                                                                                                                                                <  2:872854/237‑1 (MQ=255)
                                                                                          atatTCACGGTGGCAAAAAATATAAAACCACATTT‑TTAGTGGTAGTTTGTGGCGGTGAATTTTTTCA‑AACAAAAATAAAAAAATGAGTTGTCATTATGGGAGAAAATATAAAAAAACAGGAAGTG‑AAAAAAGTGACAGAGATTGTCGCTAACTTTACACCCCAGATTTTTACCCACACACCTGTTGACATGGGGGGTGAAATCAAAAAGCTAAAAGATGCGGGAAACTCCTAGAT‑gggg                                                                                                                                         >  2:392203/1‑239 (MQ=255)
                                                                                           tatTCAGGGTGGAAAAAAAAATAAACCCATTTTT‑TGAGTGGTGGTTTGGGGCGGTCAATTTTGCCA‑AAAAAAAAAAAAAACGGGAGTTACAATGATGGGAGAAAGTAATAAGCAAGAGGCACTG‑AAAGCGTGGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGa                                                                                                                                        <  1:827442/239‑1 (MQ=255)
                                                                                           tatTCACGGTGGCAAAAAATATAAACCCACATTT‑TTAGTGGTAGTTTGTGGCGGTGATTTTTACCA‑GACAAAAACAAAAACTGGAGTTGCCATGATGAGAGAAAGTAATAAGCAACAGGCAGTA‑AATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGa                                                                                                                                        <  1:662820/239‑1 (MQ=255)
                                                                                           tatTCACGGTGGCAAAAAATATAAAACCACATTT‑TTAGTGGTAGTTTGTGGCGGTGAATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGa                                                                                                                                        <  1:903447/239‑1 (MQ=255)
                                                                                            atTCACGGTGGCAAAAAATATAAAACCACATTT‑TTAGTGGTAGTTTGTGGCGGGGAATTTTTCCACAACAAAAAAAAAAAATGG‑GTTTGCATGATGAGGAGAAAAAATAAAAAACAGGCAGTG‑AATAAGATGATAGAGAATGTCGCTAACTTTAACGCCATGATTTCTACCCCAATGGCTGGTGACGGGGGGGGTAAACTCAAAAAAATAAAGGGAGCCGAAAAGGAGTCGGT‑gggggg                                                                                                                                       >  2:183559/1‑237 (MQ=255)
                                                                                             gacACGGTGGCAAAAAAAAAAAAACAAAATTT‑TTATGGGTAGTGTGTGGGGGTGAATTTTTCAA‑GCAAAATAAAAAATCTGGAGTTGCAATGAGGAGAGAAAGTAAAAAGCAACAGGCAGTG‑AATAAGTTGACAGAGTTTGTCGTTAATTTTTCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGa                                                                                                                                        <  2:719784/235‑1 (MQ=255)
                                                                                               cACGGTGGCAAAAAATATAAAACCACATTT‑TTAGTGGTAGTTTGTGGCGGTGAATTTTTTCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGAAACAGGCAAGG‑AATAAGATTACAGAGAATTTCGCTTACTTTACCGCCATGATTTTTACCCGAAAGCCCGATGAGGTGGGGGATAAACTAAAAAAACTAAAAGAAGCCCGAAAGTCGTCGAT‑GGGGGAAAt                                                                                                                                    >  1:871285/1‑239 (MQ=255)
                                                                                                   tggaaaaaaaaTAAAAAAAC‑CATTTGTTGTTGGTGGTGTGTGGGGGTAAATTTTTCGA‑AACAAAAACAAAAACTGGAGTTACTATGATGGGCGAAATAAATAAGCAACAGGAAGTT‑AATAAGTTCATACAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACTTCGTCGAT‑GGGGAAAATTAt                                                                                                                                 <  2:174226/233‑1 (MQ=255)
                                                                                                    tGGCAAAAAATATAAAACCACATTT‑TTAGTGGTAGTTTGTGGCGGTGAATTTTTTCC‑GAAAAAAAAAAAAAATTGAGTTGCCCTGATGAGCGGAAGGAATAAACAAAAGGCCGTG‑AATAAGTTGACAGAGATTTTCGCTAACTTTACCGCCATGATTTTTTCCCGCAAGCCTGATGAAGGGGGGGGTAAACTAAAAAAGCTAAAGGATGCCGGAAAGTCGTCGCT‑GGGGGAAATTATCt                                                                                                                               >  1:832264/1‑239 (MQ=255)
                                                                                                      gaaaaaaaaaaaaaCCCACATTT‑TTAGTGGTAGTTTGGGGCGGTGAATTTTTCAA‑GAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGGA‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTa                                                                                                                              <  1:1111938/236‑1 (MQ=255)
                                                                                                      gCAAAAAATATAAAACCACATTT‑TTAGTGGTAGTTTGTGGCGGTGAATTTTTTCA‑GACAAAAAAAAAAAATGGAGTTGCCATGATGAGCGGAAGTAATAAACAACAGGCAGTG‑AATAAAATTACAGAGATTGTAGCTAACTTTAACGCCATGATTTCTTCCCGCATGCCTGATGACGGGGTGGGTAAAATAAAAAAGCTAAAAGAAGGCGAAACGTCGTCGGT‑GGGGGAAATtatata                                                                                                                              >  1:1148268/1‑238 (MQ=255)
                                                                                                              tataAAACCACATTT‑TTAGTGGTAGTTTGTGGCGGTGAATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑ggg                                                                                                                                          <  1:691234/218‑1 (MQ=255)
                                                                                                                taAAACCACATTT‑TTAGTGGTAGTTTGTGGCGGTGAATTTTTTCA‑GACAAATACAAAAAATGGAGTTGCCATGATGAGCGAAAGTAATAAACAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCCAAAGCCTGATGACGGGGGGGGTAAAATAAAAAAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGGAAAATATTTACCATACCATGt                                                                                                                   >  1:258223/1‑239 (MQ=255)
                                                                                                                                      aGTTTGTGGGGGAGAATTTTTCCA‑AAAAAATAAAAAAACTGGAGTTGCAATGAGGAACGAAAGAAATAAGCAACAGGCGGTA‑AAGAAGTGGACAGATATTGTCGTAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                                              <  2:493407/239‑1 (MQ=255)
                                                                                                                                      aGTTGGTGGAGGTAAATTTGACAA‑CAAAAATACAAAAACTGAAGTTGCCATGATGAGCGAAAGGAATAACAAACAGCCAAGA‑AATAAGTTGACTGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                                              <  2:533339/239‑1 (MQ=255)
                                                                                                                                        tttGTGGCGGTGAATTTTTCCA‑GAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGAAATAAGCAACAGGCAGTA‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATa                                                                                                                         <  1:113038/210‑1 (MQ=255)
                                                                                                                                                        tttCCA‑GACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                                            <  1:796851/239‑1 (MQ=255)
                                                                                                                                                        gtgccA‑AACAAAGAAAAAAACTGGAGTTGCCATAATGAGCGAAAGAAAAAAACAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGTAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                                            <  2:363770/236‑1 (MQ=255)
                                                                                                                                                                           actgggAGTAGCAATTAGGAGCGAAAGAAATAGGCAACAGGAAGGA‑AATAAGTTGACAGAGATTTCCGCTAATTTCACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                          <  2:756761/235‑1 (MQ=255)
                                                                                                                                                                           aaCGGGAGTTGCCAAGATGAGCGAAAGTAATAAGCAACAGGCAGTG‑AAAAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                          <  1:733907/239‑1 (MQ=255)
                                                                                                                                                                           aaCGGGAGGTGCCATGATGAGCGAAAGTAATAATCAACGGGCAGTA‑AATAATTTACCAGAGTTTGTCCCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                          <  2:925507/239‑1 (MQ=255)
                                                                                                                                                                                              aGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAACTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTaa                                       >  1:1074648/1‑239 (MQ=255)
                                                                                                                                                                                                        ataaGCAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc                              >  1:795015/1‑238 (MQ=255)
                                                                                                                                                                                                        ataaGCAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc                              >  2:531185/1‑238 (MQ=255)
                                                                                                                                                                                                        ataaGCAACAGGCAGTA‑AATAAGTTGACAGAGATGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc                              <  1:53372/239‑1 (MQ=255)
                                                                                                                                                                                                          aaGAAACAGGCATGA‑AATAAGTTGACAGAGATTTTCTCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCg                           <  1:733017/239‑1 (MQ=255)
                                                                                                                                                                                                                cAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTc                                                                               >  1:234848/1‑181 (MQ=255)
                                                                                                                                                                                                                cAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc                     >  1:403995/1‑239 (MQ=255)
                                                                                                                                                                                                                cAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTcc                      >  1:560259/1‑238 (MQ=255)
                                                                                                                                                                                                                     cggtaaaTAAGTTGACAGAGATTGTCTCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCa                    <  2:258223/231‑1 (MQ=255)
                                                                                                                                                                                                                     aGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGCATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACt                  >  2:889578/1‑237 (MQ=255)
                                                                                                                                                                                                                       tG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGGTAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCtt              >  2:925485/1‑239 (MQ=255)
                                                                                                                                                                                                                       tG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCtt              >  2:1010254/1‑239 (MQ=255)
                                                                                                                                                                                                                            taattacgcAGAGATTGCCCATATGTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg          <  2:1148268/231‑1 (MQ=255)
                                                                                                                                                                                                                            tAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg          <  1:531185/239‑1 (MQ=255)
                                                                                                                                                                                                                              aGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAg        <  1:1014962/239‑1 (MQ=255)
                                                                                                                                                                                                                              aGTTGAAAGAGATTGTCGATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAg        <  1:391834/239‑1 (MQ=255)
                                                                                                                                                                                                                               gTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGtt                                                >  1:661725/1‑198 (MQ=255)
                                                                                                                                                                                                                                  gACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGc    >  1:251514/1‑239 (MQ=255)
                                                                                                                                                                                                                                  gACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGc    >  2:1082443/1‑239 (MQ=255)
                                                                                                                                                                                                                                    cAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGAACACCGGGGAGATTATGATCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCaa  >  2:845106/1‑239 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               
AGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTT‑TTAGTGGTAGTTTGTGGCGGTGAATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAT‑GGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAA  >  NC_000913/3206281‑3206744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A